Chapter 1458

"So director Yang decided from the very beginning to breed the sheep asexually?"

"It seems so."

"Then we're going to do asexual reproduction experiments in the future?" Su fan's tone is more and more solemn.

Wang Liangcai nodded solemnly with the same feeling and said, "it seems so."

"Are you kidding? It's not that we should... At least we should do sexual cloning first, and then asexual reproduction? " Su fan's thinking is going to be confused.

Before that, when we talked about cloning, we all talked about sexual reproduction cloning, which is to put the nucleus of the germ cell into the enucleated egg cell.

So cloning is not a new word.

But even so, cloning is still a big project in genetic biology, such as the famous juvenile fish.

In the development process of cloning, it also basically follows the process from amphibians to aquatic products, then from aquatic products to mammals, and then from mammals to economic animals.

Su fan and Wang Liangcai both feel that it's time to clone economic animals. They have a kind of self-confidence and high spirits of terminators.

However, the news of "asexual reproduction" suddenly blinded both of them.

This is equivalent to crossing several levels. It seems that children who have just started playing outdoors go directly to Mount Everest without taking a helicopter.

Most importantly, in the absence of previous experiments, biologists can not prove that asexual reproduction is really feasible.

In the normal mode, it is the easiest way to use an easier organism to prove that asexual reproduction is feasible, and then to be a mammal.

But from another point of view, what is the easier creature?

Microbes don't have to be taken into account. Bigger fish and amphibians? Their sex is very vague. It is common for female fish to become male fish in nature.

The so-called, in terms of sexual stability of the biological, at least mammals.

So is it easier for small mammals to clone? For example, the most common mice and rats in the laboratory?

No!

Wang Liangcai, who has been experimenting for a period of time, came to this conclusion as soon as his brain turned.

It takes a certain amount of time to remove and inject nuclei. The embryonic development time of mice is only two weeks, and that of sheep is six weeks. The latter is much easier than the former.

In this regard, all the animals commonly used in laboratories are not suitable for cloning. Because one of the reasons the lab chooses them is that they grow fast.

This is a good choice for the experiment with adults as the object, but when the experimental object is embryos, these animals commonly used in the laboratory are just not suitable.

Wang Liangcai glanced up at Yang Rui and said in a low voice: "director Yang chose sheep as the first choice for donation. It's a good idea to fight."

Su fan is also a smart person. After thinking about it, he knew why Wang Liangcai said so. He said helplessly: "director Yang is not a person who likes to do the project step by step."

Looking back on the projects Yang Rui had done before, from the experiment of potassium channel to the project of G protein coupled receptor, none of them were really done step by step.

Su fan and others don't know Yang Rui's real dependence. When they recall it, they can only evaluate it in this way.

Wang Liangcai could not criticize the so-called success case, but sighed and thought that young people just can't keep their temper.

However, such an idea can only be transferred in Wang Liangcai's mind. As far as Yang Rui's reputation is concerned, it has long been divorced from his age. People don't like to go step by step, that is to forge ahead

"Now what?" Su fan is not a person with special ideas. He should ask Wang Liangcai first.

"I haven't done asexual cloning. If it is feasible, the success rate will be very low." Wang Liangcai sighed.

The success rate is the eternal pain in the hearts of experimental biologists. Many experiments are well known that they can be done, and then the success rate is appalling, and finally give up.

Now when you come across an experiment that may not be able to be done, with the same low success rate, the result is disgusting - you don't know whether your failure is a misoperation or you can't do it.

At this time, Wang Liangcai is extremely eager to have a result prompt board to tell himself which way is doomed to failure.

"We have to improve our early success rate. The previous plan has to be overturned and repeated. " Wang Liangcai quickly made a decision, and said: "the success rate of denuclearization should be increased to at least 80%, and then we can do the injection."Su fan was not surprised that Wang Liangcai made this plan.

This is what the step-by-step academic school is like. It's impossible to fight for luck like Gu Qiang.

In small and simple and cheap projects on the accumulation of success rate, the last fight is the most common situation.

No one is going to change a bicycle into a motorcycle. According to the academic thinking, it's good to change a good bicycle. Besides, academic people don't ride motorcycles, do they?

Su fan didn't mean to dissuade him, so he took a learning attitude and said, "if we want to improve the success rate in the early stage, do we have a comparative experiment?"

"Yes, it's better to find a few more people for a comparative experiment." Wang Liangcai said, looking around the laboratory.

To do a comparative experiment is actually to do a small experiment.

For example, the operating solution with or without sucrose, 2% sucrose or 3% sucrose are all small details. However, the success rate of the experiment is the accumulation of such small details.

Why is egg stripping the same? Some people's success rate can be as high as 70% or 80%, while some people's success rate is only 20% or 30%. In addition to the difference in technique, it depends on who notices more details and who knows more details.

To do contrast experiment is to measure small details.

If you change to a local university and do such a comparative experiment, you can publish a paper.

A large number of Chinese journals and SCI entry-level journals publish papers of similar level. In fact, Yang Rui's computer retrieval, or academic search engine has not yet appeared, is such a data retrieval, also need a lot of human and material resources.

Su fan didn't need Wang Liangcai to assign the task. He said first, "I'll go to the information group to urge you, and put the direction in the early stage?"

"Yes." Wang Liangcai's eyes are still cruising in the crowd.

Due to the new construction of Yang Rui genetic engineering laboratory, the system adopted by Yang Rui is not the same as that of ion channel laboratory. It adopts the system of large laboratory, allowing researchers to join and form research groups freely, so it is more competitive.

However, the establishment of a research group needs funds and time. In addition, of course, it also needs research.

The $600000 + 30000 Gu Qiang won from Yang Rui, as well as the timing of the inverted phase contrast microscope, all belong to the resources of the research group.

Although the research group is still restricted and guided by Yang Rui, compared with the old model of ion channel laboratory, the large laboratory system of genetic engineering laboratory is obviously more independent and more suitable for the increasingly large-scale laboratory.

In theory, the seconded Wang Liangcai, with the consent of other researchers, will be able to recruit people. Anyway, it's good to include his name in the paper according to his contribution. This is where the main interest of the laboratory lies.

"The old Haidian District genetic engineering laboratory, sun Zhengchu researcher's level is the strongest, and the cooperation ability is also relatively good." Su fan introduced Wang Liangcai in a low voice, then turned his head and said, "in terms of experimental assistant, I think Qin Yue is very good. He used to be a veterinarian of China animal husbandry and was seconded to the Genetic Engineering Laboratory of Haidian District for a long time. As far as our current project level is concerned, it's a matter of words to transfer him."

"If Qin Yue is just an experimental assistant, it's a bit too much trouble to transfer him." Wang Liangcai is a little hesitant.

Su Fan said carelessly: "we don't have the right to use it. It's overdue. Now we are in the national project team. How do you think about the previous projects of the same level? There are only a few hundred people gathered in the whole country. Now it's time for Zhongmu to be alone. They want to send it immediately. "

Wang Liangcai was dumb. He has been doing experiments in the University before, and he has never participated in a project of this scale.

In biology, there are not many so-called big projects, and they are all in the field of medicine. Genetic engineering has always been a niche. Wang Liangcai has never tried to beat others.

"There must not be more laboratory assistants. If you want to say that, they are more scarce than researchers." Su fan paused and said, "it's hard to find a good experimental assistant. Qin Yue's words are that he has veterinarian experience, knows what's going on in the laboratory, and has participated in the project of bovine embryo transfer. He has rich experience and hard work, which is very appropriate."

There are students under Wang Liangcai's hands, but it's not appropriate to bring the people's Congress far away, and the experimental assistant is necessary.

As Su Fan said, a good experimental assistant is indispensable. Sometimes, a test tube that is not washed clean may lead to failure of the experiment, while a good researcher may not wash the test tube clean.

"Just as you said. Come here on loan to Qin Yue. " After Wang Liangcai was determined, he was in a good mood. Finally, he clenched his fist like the characters on TV and said, "let's have a big fight.""A big fight." Su fan laughed.

With their laughter, the lab became noisy.

Wang Liangcai simply stopped and said with a smile, "just go to see what happened to other people."

"Good." Su fan follows Wang Liangcai through the public area where many instruments are displayed. He sees Gu Qiang surrounded by a group of people.

Su fan instinctively frowned and asked Tian Bing next to him, "what's the matter with him?"

Tian Bing sighed: "you can't imagine that Gu Qiang hasn't failed in any step of nuclear display and enucleation since he started work. He has just done nuclear absorption of breast cells, and now he is ready to inject nuclear."

"Not a step has failed..." Su fan actually wanted to say, not a step has failed, it's no big deal, because as long as you fail one step behind, all your previous achievements will be wasted. Therefore, this is their original intention of constantly improving the success rate.

However, when the first half of this sentence came out, Su fan couldn't say the second half.

In any case, Gu Qiang's current progress is the first in the laboratory.

"If his injection is successful, this is the first successful case in our laboratory." Tian Bing came to help. He was just a spectator.

Su fan awkwardly laughs twice, just want to say something, was interrupted by Gu Qiang's roar.

"In!" Gu Qiang suddenly stood up, wild like a rabbit who completed the journey of life.

Everyone is also very happy, clapping hands, for Gu Qiang's last five seconds of blood injection and happy.

Only Su fan looked at the front, as if something happened that he didn't want to recall.