Chapter 1457

Wang Liangcai still came very early. In fact, he came earlier than he did.

The main time is adjusted to morning, so that Wang Liangcai can concentrate on the experiment without worrying about arranging time and making room. Most importantly, what he can do in the morning is not only experimental preparation and preliminary experiments, but also formal experiments.

In order to make the best use of this part of time, Wang Liangcai went to bed after watching only one episode of the dream of Red Mansions after dinner. Facing the only entertainment, he was cruel because this project was too important.

Wang Liangcai is very glad that he can be selected by this national project.

How important is the sheep cloning project?

For genetics scholars, this is basically the project of Tianzi No.1.

If cloning technology matures, all breeding and breeding will be in the past. Living in tents and forcing cattle, sheep and horses to mate will be gone forever, just like a blacksmith to a steam engine. They will still have jobs, but more just unimportant leftover work, or to save money, or for the sake of art, anyway, it is meaningless behavior.

However, no matter how important cloning is, Wang Liangcai did not dare to start the project himself.

Nothing else. Wang Liangcai's lab can't afford an inverted phase contrast microscope.

Not to mention, such a project will definitely compete with foreign counterparts.

Even if you don't say what you are doing, foreign scientists engaged in genetic science are approaching this goal step by step.

As long as you think about the world's leading genetic engineering laboratories, which are likely to set foot in, Wang Liangcai feels that the pressure is doubled.

That is, in Yang Rui genetic engineering laboratory, Wang Liangcai's mood can be calmer, and he can do experiments with ease, without considering so many miscellaneous things.

For Wang Liangcai, this is the state he has been longing for for for a long time.

Compared with that, watching one episode of dream of Red Mansions less every day, going to bed a few hours earlier and getting up a few hours earlier is nothing.

It's not like this before the entrance examination.

What teenagers can muster up courage and perseverance to do, can't people do near middle age?

That's the more you live, the more you go back.

Led by Wang Liangcai, many researchers in the laboratory came earlier than usual. The most obvious is Su fan.

Now, Su fan is an old man in the ion channel laboratory.

When Yang Rui dug up the calf, he was one of the first to dig up the ion channel laboratory. Later, he set up his own research group to do cloning related research.

It should be said that his research group still has a very good output. When he was doing bovine embryo transfer in Haidian District genetic engineering laboratory, he gave considerable help.

After that, Su fan joined the G protein coupled receptor project and undertook a lot of work.

No matter in which period of evaluation criteria, Su fan is a researcher with small achievements.

However, what I fear most is comparison.

Su xiankai, who joined the ion channel laboratory at the same time as Su fan, started the project of G-protein coupled receptor independently. Su fan joined in later, not even as a deputy to Su xiankai, but as a three leader and four leader after fan Zhenlong and Gu Qiang.

Others may be able to bear it, but Su fan can't bear it at all.

Su fan, who graduated from Beiyan agricultural college, has always been sensitive to differential treatment. Maybe she suffered more discrimination, so from a long time ago, Su fan was very concerned about other people's vision and arrangement.

Academic discrimination can be regarded as the most fruitful one. To break its shackles, we have to separate education and ability.

Emphasizing one's own ability, thus proving that education is just a result of playing when one is young and ignorant, which is a common means when one is discriminated against by education.

So is Su fan.

It was not until the previous unit tore the fig leaf of the ability to determine the title that Su fan moved to the ion channel laboratory.

In the ion channel laboratory, Su fan had a comfortable life for some time, but psychologically, his pressure did not ease.

The prefix of ion channel laboratory is Peking University. Therefore, what Su fan sees most in the laboratory is a researcher with a degree from Peking University. Yang Rui, Xu Zhengping and others did not say that later Jiao Yangping, Wang Sisheng and others came from Tsinghua University, and then came Professor man Hai, Professor Feng Junming and Professor Tao Xuelin, who also came from Peking University, which greatly touched Su fan.

Some time ago, the work was too intense, so Su fan did not have time to think about all this carefully.After he was sent to the genetic engineering laboratory, Su fan's heart of comparison was overwhelmed.

Especially compared with Gu Qiang, Su fan does not agree with the latter.

Gu Qiang is not a graduate of a famous university. Why should he bring a research group?

Although Gu Qiang has completed many experiments in succession, Su fan still wants to compete with him.

Of course, cultural people can't challenge directly. It's too ugly. Su fan decided to learn from Wang Liangcai and take Gu Qiang as the first step to do the task of cell enucleation.

Learning is actually helping Wang Liangcai.

If not, Su fan does not have the confidence to complete the work of cell enucleation.

Unlike Wang Liangcai's long-term training, Su fan himself has not received the practice of "egg stripping". Beiyan agricultural college does not teach this. After he joined the work, he could not find anyone to learn. Therefore, the independent experiment is totally self inflicted.

Su fan not only holds the mentality of stealing teachers, but also holds the attitude of helping, and begins to give Wang Liangcai a hand. He wants to study for a while, and then consider whether to restart the stove or open up a new way.

However, the technology Wang Liangcai mastered obviously exceeded Su fan's expectation.

We all peel eggs. Gu Qiang's ability to peel eggs is basically enough. It's just that he can peel them off.

Wang Liangcai's standard technique has been practiced for many years, and there are too many places to learn.

After Wang Liangcai ran for several mornings, Su fan got up earlier and earlier.

The interaction between the two is not unexpected in Gu Qiang's eyes.

If you don't worry at all, it's a bit fake. As members of the same research group, there is also competition. At least, when future papers are published, we still have to fight for one.

But it's no use worrying. All of Tong Dizhou's disciples and grandchildren are egg strippers. Gu Qiang, who was born in Shanda, has strong experimental ability, but in terms of egg peeling alone, he really has no advantage.

As the days went by, he saw that Wang Liangcai and Su fan cooperated with each other more and more tacit. They also went from the nuclear test to the de nuclear test. Gu Qiang could not sit still any more.

"Director Yang, I want to change an experimental idea." Gu Qiang's research group can only carry out the experimental operation within the specified scope, and the change of thinking must be approved by Yang Rui.

Yang Rui is well aware of the recent laboratory atmosphere, but he is so happy. He does scientific research three hours a day, eight hours a day and eighteen hours a day. Whether there is competition or not is very different.

The benign competition in the laboratory can't be better. Yang Rui was not surprised to ask, "how do you want to change it?"

"I want to do a one-stop experiment. I don't want to do it step by step." Gu Qiang is also a killer.

In fact, the steps of cloning are quite simple. The core part is to remove the nucleus of one sheep's egg cell, insert the new egg cell, and then insert it into another sheep. The lambs born are cloned sheep.

Although there are many steps before and after, such as superovulation, such as taking care of pregnant sheep and so on, any step is indispensable. However, there are not many core steps.

Doing step by step is not only practice, but also a way to find a higher success rate.

Like the last step, you have to let the ewe give birth to a healthy lamb. If you can't find ways to improve the success rate, the previous steps are not wasted.

When the Scots were doing sheep cloning in those years, they failed three times in the last step. Every time they were a few months old, you can understand the hard work.

However, the formal method is not particularly suitable for Gu Qiang.

He is not an experimental biologist with a good theoretical level, but more like a technician in a foreign laboratory. Although the latter also plays theory, they all play specific operations. The difference between the two is just like the difference between an automobile engineer and a mechanic.

An excellent repairman is sometimes more powerful than an automobile Engineer in refitting a car. However, if you want to develop new technology step by step and improve the technical indexes of every part of the car, you still have to be an automobile engineer.

Gu Qiang also knows his own shortcomings. After a period of time, it is difficult for him to improve the accuracy rate in a single link. It is not the problem of experimental techniques, but the problem of small ideas in small experiments.

Therefore, Gu Qiang simply put forward a one-stop plan.

Yang Rui can't help but be silent. He also knows Gu Qiang's problems. However, the reason why step-by-step is a formal method is that when step-by-step, the overall success rate is higher. Compared with one-stop, it costs less resources, gets more achievements, has a higher repetition rate, and more importantly, it saves more time.A sheep pregnancy must be a few months, you go through one-stop, one failure is equivalent to a year's time, and how many failures.

However, considering Gu Qiang's strong fortune, Yang Rui hesitated.

If according to the standard of academic judgment, Gu Qiang's level is not as good as Wang Liangcai's.

In terms of experimental ability, Gu Qiang is also first-class in China, but Wang Liangcai is at the top of the Chinese Academy of Sciences. In terms of domestic scope, those who are better than him in technology, are over aged, and those who are younger than him are far behind in theoretical level and experience

It can only be said that Gu Qiang lifted a stone and hit himself on the foot. When he spoke, he named Wang Liangcai, I didn't expect that other people's skills were all over themselves.

Except for strong luck.

When he thought of the word again, Yang Rui couldn't help looking up at Gu Qiang, who grinned awkwardly and politely.

"If it's a new one, everything will change. Are you ready? " Looking at Gu Qiang's breakthrough achievements in several scientific researches, Yang Rui is still relieved.

Gu Qiang even said, "I've finished the report."

"Where?" Yang Rui stares at him. He doesn't send something. He says what's going on.

Gu Qiang quickly went back to take it and handed it back to Yang Rui. He said with a dry smile, "I didn't expect you would agree."

"You mean I shouldn't agree?"

"No, no..." Gu Qiang giggled again. He originally thought that Yang Rui would have to bargain, for example, breaking one dragon into two dragons, which could reduce the cost and risk, but he didn't think that Yang Rui wanted to bargain.

"Expected funding of RMB 6 million plus US $300000?" Yang Rui laughed when he saw the final figures in the report.

This is also in his laboratory. Out of Yang Rui's laboratory, there are only a few laboratories in the country that can give this funding.

Of course, Yang Rui's laboratory itself is one of the few high-end laboratories in China, but the amount of funding is exaggerated.

Before that, Yang Rui did a big project, and the total funds fluctuated up and down in this figure.

It can be said that in China, the fund of 10 million yuan is the ceiling. What can reach this figure is not the result or reputation. You can only compete for this kind of fund value if you get great achievements, have great reputation, trust, good resume, and find the right project.

Gu Qiang is far away from such qualification.

With a wave of his hand, Yang Rui removed the zero after six million and three hundred thousand, and said: "600000 plus thirty thousand dollars, there is almost one million dollars. It must be enough to buy consumables."

"I also want to buy some instruments..."

"Make something." It is impossible for Yang Rui to allocate more funds to Gu Qiang alone.

If you want to say that, one million is too much.

He only leads a research group, not a project group. All the honors go to the master. Therefore, he can't decide the major expenditure of purchasing instruments.

However, if Gu Qiang can compete with Wang Liangcai this time and make things by one train, it will be different.

It's also very good for Yang Rui to set up another project team in the laboratory.

Scholars can't be emperors. The most important thing is to be a Northern Warlord. There are factions in the warlord. They are not only normal but also healthy. On the contrary, those who want to be authoritarian eventually lose their reputation.

Gu Qiang didn't think as much as Yang Ruiyuan. He was not satisfied with the $600000 plus $30000 fund. He bit his teeth and said, "you can see, the progress on my side must come from behind."

"Is that behind?"

"No, it's not backward." Gu Qiang quickly pleaded: "my success rate of nuclear display is almost 50%, and the success rate of nuclear removal is not low. I just feel that it's too boring to do this step by step..."

His voice is getting smaller and smaller, because his success rate is lower than that of Wang Liangcai.

"Director Yang, shall I go back first?" Gu Qiang felt guilty when he saw that Yang Rui was still reading his rough and rotten report.

"Wait a minute." Yang Rui stopped him slowly and said, "your nucleus injection part is to use the nucleus of blastocyst stage embryo?"

"Yes, do you think blastocyst stage is not suitable?" Gu Qiang is open-minded to ask for advice.

In the process of embryo development, there are many stages. It is very common to use blastula cells.

But Yang Rui shook his head and said, "I have no problem with blastocyst stage, I have problem with embryo.""What kind of sheep do you want?"

Yang Rui shook his head again and said, "my idea is to try non fertility cells, such as breast cells and other somatic cells?"

Gu Qiang station is not stable: "the nucleus of somatic cell, how can it be used to inject nucleus."

"How do you know if you don't try." Yang Rui said, "didn't I mention it at the project description meeting?"

"I thought it was a vision." Gu Qiang's heart is still palpitating, and more of it is denying.

"Since you have decided to do a one-stop experiment, don't wait," Yang said

"This..." Gu Qiang repented.

Before the birth of cloned sheep, including Chinese biology textbooks, the specificity and totipotency of biological cells were listed as important theories.

In short, according to the current understanding, a cell is omnipotent during the embryonic period, that is, it can do anything in the future.

The cells that differentiate into skin tissue, blood and heart are specific. In theory, they can't grow in reverse. They don't differentiate what they want, like embryos.

Take childbearing as an example, that is to say, there are only a few people who are fertile, and the rest can not be used for childbearing.

Dolly, the cloned sheep, is really breaking the shackles.

Perhaps for ordinary people, what cell nucleus is not a note? But for biologists, it's a radical change.

It's not too much to say that the biological building collapsed.

Although the biological building has fallen year by year, this time it has fallen more severely.

Therefore, although Yang Rui began to elaborate his ideas from the beginning of the project, including Gu Qiang and others, he still regarded it as a scientific fantasy.

And fantasy into reality, sometimes is beautiful, sometimes more difficult than nightmares.