Chapter 1334

"Director Yang, what's the situation here?" Pang Jiancheng asked Yang Rui with a smile, thinking: the acting skills are quite good, Peking University is really a hidden dragon and crouching tiger.

No one thought that new research results would come out, and Pang Jiancheng naturally didn't expect that. As long as it was arranged by Yang Rui, he gave him a big hand.

On the contrary, a few foreigners, listening to the translation of the "three-dimensional structure", whispered.

Yang Rui hesitated for a moment, coughed twice, and said, "it seems that there is good news coming out of our laboratory. Let's wait for a moment."

With that, Yang Rui followed the student back to the laboratory and asked for the photos.

Gu Qiang and a group of people gathered around the electron microscope with different looks.

"I just wanted to be idle, so I took a few photos, but I couldn't use them. Scholars in the 1980s knew little about the three-dimensional structure of GPCR, which gave Yang Rui a comparative advantage.

He only looked for a while, put the image back in the bag, and then looked at Gu Qiang with a scanning eye.

"Director Yang..." Gu Qiang was a little guilty and called out.

"Did you record the experiment?"

"I did." Gu Qiang quickly took it out. It's a good habit, and it's also a place where the ion channel laboratory is better than other laboratories. Even if it's a casual experiment, you must make experimental records and keep notes while doing it.

Experimental records are useless in most cases, but when you find something, especially the world-class discovery, your only regret is that the experimental records are not detailed enough!

"I wish I had... At the beginning" is a common saying of people who have not received systematic training.

The purpose of systematic training is to prepare researchers to seize every hard-earned opportunity. After all, the number of times a person steps on a bicycle in his life is limited, except Gu Qiang.

Yang Rui glanced at Gu Qiang again. After reading the experimental record, he said, "there is no problem for the moment, but I still want to see the repeated experiment."

"Of course." Gu Qiang finished and said in a low voice, "in this case, will the three-dimensional structure be made?"

Yang Rui hesitated and said, "basically."

"Yes Gu Qiang gave a low cry.

"We have to improve the rest and draw the three-dimensional structure, but that's basically it. The rest is rough work." What Yang Rui said also has some feelings.

For the third stage experiment, namely the three-dimensional structure of GPCR, the most important and main demand is to find the activation mode of small extracellular ligands and GPCR.

In short, G protein coupled receptor is a kind of protein, although it can not be seen by human eyes, but on the molecular level, it is very huge. It's like a molecular building, with people and cars coming in and out.

These people or cars are extracellular ligands.

For example, how does the sense of smell come into being?

There are all kinds of molecules in the outside air. When they enter the human nose, some of the molecules that can be distinguished by human beings will come into contact with a certain G protein coupled receptor in the human body. This molecule is the pedestrian who enters the G protein coupled receptor building.

When pedestrians enter the building, the building sends a signal to the brain, which searches for information and tells people what you just smell.

To delineate the three-dimensional structure of GPCR is to describe the appearance of the building. The most difficult part is not the outline - the outline of the huge GPCR is very easy to make. The most difficult, important and important part is to see how it accepts pedestrians and vehicles.

Specifically, it is to look at the position and shape of various openings on the G protein coupled receptor molecule.

In other words, what human beings want to know is where the elevator of this GPCR building is, where the garage door is, where the front door, side door and back door are, and whether there is an overpass and a rooftop.

Using instruments to scan G protein coupled receptors is like using a laser to scan a building. They don't really see everything in the building, but need to rely on all kinds of feedback to get the answer.

It's like shining a laser on the door of a building. If the door is closed, the laser will give a reflection and get the wrong answer that there is no way here.

The scanning of G protein coupled receptor is more complex, because the protein is mobile. When it has a relationship with the extracellular ligand, it will show the necessary three-dimensional structure. Otherwise, it is a closed protein.

To say, G protein coupled receptor is often exchanged with the outside world, but it is a great test of luck to seize such a moment picture.In history, it took Kirby Carr about four years to break out of character and get the picture he needed - the moment when an extracellular ligand touched the G protein coupled receptor.

It took Gu Qiang about four months to do relevant research.

Dog @ Shiyun's victory over hard struggle has happened countless times in history, and it will not be the last time.

It's just a coincidence for Yang Rui.

"You continue to repeat the experiment and write down all the experimental records you can remember." It's impossible for Yang Rui to give up this achievement. The next time he takes the picture he wants, who knows what century it is.

However, Yang Rui should not let these achievements be exposed to the Swedes unprepared.

No scholar can resist the temptation of Nobel Prize.

"Who can take the place of repeated experiments?" Yang Rui raised his voice and asked.

Several researchers looked at each other, and finally Su xiankai stood up.

Yang Rui nodded and said, "now, let's distinguish the second stage experiment from the third stage experiment. Don't move the electron microscope. Tian Bing, you can help Gu Qiang to make a record."

It's a normal step to complete the record before repeating the laboratory.

After a little confusion in the laboratory, Yang Rui took another look at the electron microscope. Although he hesitated, he went out.

"Ladies and gentlemen, we have just completed a new round of repeated experiments to prove that we have obtained the crystal structure diagram of the ligand binding complex of the N-terminal ligand domain of GPCR." Yang Rui used the tone of announcement to face a group of Swedes who were spitting out by the hot pot.

……