Chapter 1197

Yang Rui has been sleeping in the ion channel Laboratory for several days.

It's not that he wanted to fight out of his physical strength from the beginning. However, Su xiankai, fan Zhenlong and Wang Sisheng were so enthusiastic that he had to work overtime.

In fact, if we enter the middle of the experiment, the overtime work of the three people may not affect him. The scientific research ape is very tired, and the feeders travel around the world to give lectures. However, the early stage of the experiment is different. There are so many things that Yang Rui needs to confirm that there are so many problems for the three that Yang Rui can't avoid.

Follow the prescribed order. If follow the prescribed order follow the prescribed order, he will be able to work on a regular basis. He can take the vacation as if he were on a regular basis. It is like the rancher can enjoy the freshness of the air and the taste of steak, in the condition of the wind and rain, the awesome cowboy and the nothing around. A cowboy is as plump as a cowboy or a sheep.

However, there is little wind and rain, awesome cowboys and nothing around.

Especially for a new ranch, it's a rush of business. Just to confirm the purchase of reagents, Yang Rui was busy for an afternoon.

If the G-protein coupled receptor group runs smoothly, such as purchasing reagents, Yang Rui can completely ignore it. It's just a routine laboratory life. It's just as easy as buying gloves of what brand and drawing paper of what type.

But at the beginning, Yang Rui really can't take these small problems for granted.

The simplest way to buy reagents is to pay attention to compatibility. For example, it seems easy to buy domestic reagents or imported reagents. If you have money, you can buy imported ones. If you don't have money, you can buy domestic ones.

For the research dog who graduated in two or three years, maybe the answer is so, but from the perspective of the project leader, the problem is quite different.

What Yang Rui needs to determine at the beginning is actually the reagent needed for the whole experimental cycle. Otherwise, if you use imported reagents in the early stage and domestic reagents in the later stage, and then compare the two, the result curve will be in a mess. In this kind of high standard international scientific research competition, articles are aimed at CNS, and the pictures in it are to be read and even experimented repeatedly. Chaos will destroy a good data.

Moreover, the purchase of reagents is not limited to the difference between imported and domestic products. Yang Rui's final answer on paper is not only to decide which country, but also to decide which company, which factory and even a certain batch.

The purity of reagents can be selected from superior purity, analytical purity, chemical purity, chromatographic purity, etc., which correspond to the number of impurities. However, for some reagents, no matter how high the standard is, it is still impossible to completely eliminate impurities. For experimenters, this is a very annoying thing. You do not have the conditions. How can I do the experiment?

Unfortunately, it does not mean that scientific research can be carried out without the actual conditions. The correct way is to ensure the consistency of impurities. The impurities of the same batch of reagents in the same factory are usually consistent. In this way, through comparison and calculation, the influence of impurities can be eliminated to a great extent.

Extending the same approach, we can draw two conclusions. First, even if it's a high-purity experimental reagent, it's good to buy a batch, because you don't know if the other factory will produce a batch of substandard reagents, which will make you meet. It's not prudent and unprofessional to put one's own years and millions of dollars in other people's prudence and professionalism.

Not to mention, scientific research is doing small probability things.

The second conclusion is that relatively demanding research can be carried out with reagents of lower purity. As long as the data is processed in the later stage, which is completely in line with the rules, the results will be similar to the experiments with reagents of medium and high purity. The main reason why Chinese laboratories have always done this is that they are poor.

In addition, the more pure the reagent, the better.

The first problem we face is naturally whether the purse can stand it. If the purse can stand it - in extreme cases, there are also examples of not buying high-purity reagents. For example, those who make materials often like to buy domestic reagents, because domestic reagents often have inexplicable impurities, which can bring surprise to people.

In the history of science, there are so many examples of the so-called meritorious service of impurities, which became popular at the end of 16 and the beginning of 17.

Secondly, it's not about buying expensive reagents.

You can't buy analytically pure concentrated sulfuric acid for waste liquid treatment. Even if you are rich in running oil, it's too hard to dismantle the analytically pure small packaging bottle and pour it into the waste liquid bucket.

It's totally thankless.

In addition, there are no really rich people who do scientific research on the earth. Take the biological laboratory in 1986 as an example. You can say how many tons of interferon you want for a gram of US $50 million.G protein coupled receptor is originally a money burning project. It is enough to start with 6 million US dollars, but it must not be wasted at will. Especially in the early stage, the establishment of standards should be more careful.

You can't go to a double base right now because the fog of war is open.

Many of the reagents used in the early stage have to be used all the way to the end of the project. If the project has to be done for three years, five years, or even five years and eight years, it is impossible to suddenly change to cheap reagents in the fourth and seventh year, and the value of the experimental data in front of it will be greatly reduced. Therefore, instead of gnashing teeth at that time, we should establish our own standards in the early stage.

This is also a challenge for Yang Rui.

There is no one to teach us how to plan for the beginning, and there is no definite number. The world is changing too fast. To some extent, we need to make some predictions. Such predictions not only need to predict the time of our own projects and the expected funds, but also predict the state of our competitors and the trend of scientific research.

Of course, the most important thing is experience.

In developed countries such as Europe and the United States, Yang Rui can also try to consult other top laboratory scholars. Unfortunately, there are no corresponding conditions in China.

It's Professor Cai and others. Although they have made international achievements, they have not set up international laboratories. Under the condition of carrying out international projects, they have obtained international achievements.

In sum, since the artificial synthesis of bovine insulin in China, there has been no such targeted large-scale biological research and development. In the last two or three decades, there have been few scientific researches in biology that have been well prepared for war, and then participated in positive international level scientific research competition. Basically, it's ambush, special operations or secret service teams behind enemy lines.

Back then, when the Institute of Biochemistry, Peking University Department of chemistry, and Institute of organic chemistry of the Chinese Academy of Sciences assembled a large number of troops, organized the distribution of assault suits, led the mobilization before the war, and opened the artificial synthesis of bovine insulin with a scalpel, science and nature were published one after another, Although it is only an American laboratory and a German laboratory that are fighting with the strength of the whole country, this is indeed the highest level of confrontation in a specific scientific research field. To say that it may not be possible to shock the world, but it is true to shock China.

However, that was 30 years ago.

Therefore, Yang Rui has no place to consult.

However, from a good point of view, it also makes Yang Rui have no competitors and less expert guidance.

"Anyway, it's my own money. I'll pay my own tuition..." Yang Rui gritted his teeth and signed on various documents and projects.

The US dollar account in the people's Bank of China is also the decline of brushing. The distressed branch business managers all called to inquire.

10 Ten thousand dollars.

20 Ten thousand dollars.

50 Ten thousand dollars.

100 Ten thousand dollars.

200 Ten thousand dollars.

By the time the preparatory work is completed and the instruments and consumables to be used in the early stage are ordered, 2.7 million US dollars will have gone out.

Looking at the figures released inside the laboratory, Su xiankai was very bored at that time. In a flash, he was in a cold sweat on his back.

"Fortunately, you didn't listen to me at that time." Su xiankai started from the beginning to the end. He knew that Yang Rui didn't choose a particularly expensive product. He basically looked for it in the lower middle range. It belonged to a more frugal opening mode. It didn't mean that he could no longer be frugal. However, if he continued to be frugal, he could only do tracking experiments and was not qualified to participate in scientific research competition.

However, by such a standard, the total amount of a general election was US $2.7 million, almost three times what Su xiankai wanted. Su xiankai was also afraid of it.

There's no turning back. One million dollars has been spent. If you don't see the sound, you can't get it back.

Yang Rui didn't feel lucky either. He just said, "one success is enough. The rest of the money can make people happy. Maybe there are one or two seven figures left..."

"Director Yang, please don't stir us up. We haven't even found the door." Fan Zhenlong can't laugh any more. Recently, just a purified G protein, let him and Wang Sisheng practice the whole set of experiments.

Su xiankai, a skilled worker who has purified a group of G proteins, has no confidence. He said: "if we can achieve rhodopsin, there will be another success. I already feel a little bit about it. However, your requirements must not be so low."

Yang Rui turned his eyelids and said, "bovine rhodopsin is the experimental material. It's not in the experimental steps yet. Lao Fan, what about you? Is the detergent easy to use? "

Detergent can increase the solubility of G protein, which is the best way to purify G protein at present. It is also a secret book in high-end laboratories and has not been widely spread.But fan Zhenlong still shook his head and said: "it's easier to use, but we haven't found the trick to extract the receptor from the cell membrane. Lao Su doesn't understand. "

Yang Rui naturally looks at Su xiankai.

Su xiankai blushed and said: "Lao Fan, what they choose to purify is GT, and I have never been in contact with it. Let's start again. We can't make sure of all kinds of doses. In fact, I don't have any tricks. I just do a lot of work. It took me a year to purify it. "

G There are many kinds of protein and G protein coupled receptors, and there must be a certain amount to make a systematic comparison. Therefore, although purification is for fan Zhenlong and others to practice, it is also one of the necessary conditions in the early stage of the experiment.

But if we have to wait another year and a half to produce one more purified protein, the laboratory can't afford to wait.

Yang Rui could not help but bow his head and ponder.

The secret of purifying G protein with detergent is also from his book, and he has never operated it on the spot. Moreover, there is no specific experimental guidance in terms of his experimental level.

In any case, fan Zhenlong and other calves are better than him in their experimental level. However, their future success does not depend on their experimental level. In terms of their experimental level, they are afraid that they are not as good as Gu Qiang.

Thinking of this, Yang Rui patted his head and said: "in this way, I'll add another farmer to you... No, I'll add an experimental assistant. It's Gu Qiang from the genetic engineering laboratory. He has strong hands-on ability. You can cooperate with each other."

Learning also needs help. Since the speed of idle mining is slow, it's better to add a farmer.

Anyway, the genetic engineering laboratory is also under his name, and they are all public laboratories. It's very convenient to borrow something.

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