Chapter 833

The positive contact of the Americans, especially darbeco, has greatly stimulated Zhang Leshan and other five groups.

Zhang Leshan and others, in order to welcome the arrival of Americans, did not hesitate to increase the funding for PCR related experiments.

For example, director Zhao's team even increased expenditure and manpower in order to improve speed.

The Biology Department of the whole department is thriving. Every time President Cha comes out of the office to inspect, he is deeply gratified by the vitality of his newly established laboratory. He just feels that the whole scientific research system seems to be brought alive by himself.

Director Zhao and others also feel very cool. Compared with doing research, repeating experiments or reverse engineering, people always feel more comfortable.

Innovation is like an old forest that no one has ever been to. You may not get anything if you go into it. The only sure thing is that you have to work hard to get out, or even never get out again.

Repeated experiments or reverse engineering is simple. It's like traveling to a famous mountain and river. You can basically determine what you can get when you go in. The only thing you need to do is to work hard and make some efforts. Maybe you need to give some tickets.

Compared with the deep mountains and old forests, the famous mountains and rivers developed by predecessors are more comfortable. It would be better if tickets and travel expenses could be saved.

Director Zhao and others' cheerfulness lasted at least half a month. By the middle of February, director Zhao's own team had problems first.

"According to the literature, the high temperature resistance of the polymerase can only reach 45 degrees at most, and it can't be improved any more." Zhu Guanghui, the researcher in charge of the experiment, reported to Director Zhao.

"Didn't you do it again?" Director Zhao, who just came back from DongLaiShun, was full of oil and couldn't take care of the taste.

"I did it again and isolated the strain again, but after it was made, the high temperature resistance was... Improved twice." Zhu Guanghui was quick and anxious. When he talked about the specific temperature, he lowered his speaking speed and was also anxious.

Director Zhao frowned tightly. After a while, he said, "45 degrees, too bad."

Zhu Guanghui blushed a little, and immediately straightened up his chest to explain: "I know that 45 degrees is lower, but compared with our previous research on thermophilic bacilli, the temperature has increased a lot."

"Before Yang Rui made heat-resistant polymerases, the best heat-resistant polymerases could not reach 40 degrees, but now we have increased by at least 20%." Another researcher nearby also said it.

"But Yang Rui did it above 90 degrees." Director Zhao only felt the tension between the teeth was tight, like there was shredded meat stuffed in.

Zhu Guanghui's face turned red. He had been afraid that director Zhao would say this before, but now he finally heard it.

"More time, we should be able to improve." Zhu Guanghui's voice was like a barbecue, low and thin.

"How much?" Asked director Zhao.

"50 degrees should be OK."

"50 degrees doesn't make any sense." Heat resistant polymerase is the raw material of PCR instrument. It must be able to endure 96 ℃ high temperature. The polymerase below this temperature is just ordinary polymerase.

If there is no heat-resistant polymerase, then we have to go back to the era of manual PCR, and do DNA replication like spicy hot.

It can be said that heat-resistant polymerase is an important part of PCR instrument. It can even be said that with heat-resistant polymerase, there was a generation of PCR instrument. PCR technology without heat-resistant polymerase is only a usable technology, which is called pcr0.1 at most.

Director Zhao stares at Zhu Guanghui with serious eyes.

Zhu Guanghui stumbling way: "we still need time."

"We have time. How long do you need?" Director Zhao's expression did not ease. He knew that there must be problems. Otherwise, the atmosphere of the laboratory would not be so good for the first half month, especially after the official start.

As expected, Zhu Guanghui said, "we have found several unexpected situations. Now, before we overcome the difficulties, we can't determine the specific time."

"One more month, what can you do?"

Zhu Guanghui whispered: "one month is not enough."

"A month and a half?"

Zhu Guanghui shook his head slightly.

"Two months?"

Zhu Guanghui's head drooped.

"You say a number."

Zhu Guanghui thought for a long time and said, "half a year...""Can you do the same thing in half a year?"

"Maybe we can make something that works." Zhu Guanghui's voice was a little lower.

Being able to use and being the same are two concepts. As an enzyme, thermostable polymerase has the best working temperature. The so-called thermostability usually means that it can also work at high temperature, that is, it can be used. However, it is very difficult to achieve the best performance at high temperature.

Yang Rui's preparation work is sufficient, especially the direction is clear, in order to make a good use.

But for director Zhao's experimental group, the problem is not so simple.

However, as the most important raw material of PCR instrument, whether the heat-resistant polymerase is good or not will greatly affect the effect of the final PCR instrument.

Director Zhao said in a deep voice: "just maybe? Two days ago, you were very confident

"We didn't expect that as the temperature increased, the number of problems we encountered increased geometrically." Zhu Guanghui is facing depression.

Director Zhao black face, said: "let me see."

Zhu Guanghui busily took out the experimental records, and then explained several key points to Director Zhao.

Director Zhao is in charge of the laboratory, and his scientific research level is not weak.

After a while, director Zhao found that Zhu Guanghui's judgment was correct.

"That's the trouble." Director Zhao shook the experimental records, thinking about the funds and time.

Although it is reverse engineering, and there are references, but the specific experimental steps, materials and details are not clear, there are still countless kinds of cross possibilities.

Director Zhao's laboratory is not as good as Huarui in terms of conditions, funds, direction and guidance rules, and the time they invested is far less than that of Huarui. It's only natural that they can't do it.

Similarly, traveling to famous mountains and rivers, if potassium channel related research is climbing Huashan Mountain, it is about the same as climbing Yulong Snow Mountain, which is not as dangerous as Huashan Mountain, but it is just as hard and dangerous.

Such a tourist area snow mountain, well prepared, healthy people are able to climb up, the difference is nothing more than time and energy input.

Yang Rui spent half a year preparing and half a year doing it. Huang Mao is a maverick in practical operation. Of course, his current level is not up to that of the Mavericks, but his experimental ability is comparable to that of the Mavericks.

Director Zhao underestimated, only when Taishan to deal with, will naturally estimate the wrong time, causing delay.

"Director, what should we do now?" In the lab, a researcher asked questions that everyone wanted to ask.

In a laboratory, the biggest and most important person in charge usually doesn't have to do experiments in person, just like the biggest and most powerful executive chef in a hotel, who usually doesn't have to be in charge.

In addition to obtaining projects and funds, the person in charge of the laboratory is mainly to determine the direction and solve problems, just like the executive chef, who mainly decides how to cook a dish and what to do when encountering problems.

In addition, he naturally has to supervise the time and expenses, just as the executive chef has to tighten the speed of serving and limit the use of raw materials.

Usually, the laboratory director can eat DongLaiShun if he wants to eat DongLaiShun, but when the problem arises, he has to make a decision.

Director Zhao is full of paste.

There was no heat-resistant polymerase in China before.

Before PCR, this was originally a less important branch of scientific research.

Therefore, from the perspective of scientific research, we have made heat-resistant polymerase from scratch, and raised the temperature of heat-resistant polymerase to more than 40 degrees, which is a very good achievement, enough to fill the gap in China - and so on, the gap in China has been filled by Yang Rui, which is one of the reasons why some people don't like Yang Rui, To make an innovative achievement abroad and copy it at home is to fill the gap. Now, Yang Rui has made an innovative achievement, but it's useless to copy it at home.

Therefore, before the scientific research circle invents a new word, the classical expression of filling the gap in China will not be easy to use.

As far as heat-resistant polymerase is concerned, Yang Rui actually made high-temperature resistant polymerase materials several years in advance, but director Zhao could not provide the capital he invested.

"I didn't expect that..." director Zhao fell into a deep meditation.

"I didn't expect that..." several scientific research groups were lost in meditation.