Chapter 794

More than 30 compounds used in animal experiments were synthesized step by step by Yang Rui.

Naturally, Yang Rui is also clear about the outcome of these compounds.

In the final analysis, Yang Rui only needs one or two compounds, no more than four at most. The remaining compounds will be eliminated.

If we want to speed up, the best way is to let the eliminated compounds be eliminated earlier.

The reason why the screening of active substances is getting slower and slower is naturally that the remaining compounds are becoming safer and safer. Those obviously unsafe compounds, such as those with acute toxicity, have been brushed out in several initial tests.

But in the next test, those unsafe compounds will eventually be eliminated, just a matter of time.

The problem is that Yang Rui doesn't want to wait that long.

Some tests will only be carried out after a long time. Some tests are not even needed in the 1980s, but started in the 1990s.

But as a new drug developer, considering the situation ten years later, it's just too normal. Ten years later, new drugs may not be on the market. Even the drugs on the market may be withdrawn.

For example, a large number of drugs withdrawn in the 1990s are metabolized by mixed function oxidase, which is no problem in itself. However, patients often do not take a single drug, but they will use a variety of drugs together. As a result, these drugs metabolized by mixed function oxidase are likely to have cross reactions, and the consequences are as follows, In animal experiments, there is no test or the final test.

Because drug cross reaction is not the focus of animal experiment.

But from the point of view of whether a drug is successful or not, drug cross reaction can not run away.

Similarly, there are genotoxicity problems, metabolites problems, pharmacokinetic characteristics, and even whether the raw materials are easy to obtain, whether the synthesis cost is reasonable and low.

These problems are not considered by ordinary people, but must be considered by pharmaceutical enterprises. For example, cold medicine in 10 years' time will not be considered as raw materials. Some raw materials that need to be closely monitored have such problems.

If you want to think about it, you can also understand that if you want to buy a box of cold medicine, you need to check your ID card, and limit the quantity of it. As you can imagine, in Europe and the United States, it is equivalent to turning over-the-counter medicine into prescription medicine. In order to reduce the sales by several times, pharmaceutical enterprises should be cautious.

In a word, all the work of pharmaceutical enterprises is to be cautious.

Whether a drug can become a clinical candidate compound after the end of animal experiments means that pharmaceutical companies need to spend $20 million to $200 million and wait four or five years for clinical research.

This amount and time is enough for two countries to fight a local low-intensity war. How can pharmaceutical companies be careless.

What Yang Rui needs to do is to eliminate the compounds that are destined to be eliminated as soon as possible, so that he can concentrate his time and energy on a few compounds, and then make a detailed test and analysis.

In the end, if these few compounds can meet the requirements of clinical candidate compounds, even if Yang Rui can't personally promote them to go public, pharmaceutical companies will actively do it.

The only trouble is that Yang Rui needs to spend more time to participate in such elimination work, not vice versa.

Of course, it will take some valuable time.

However, Yang Rui also feels that he needs such an experience. Desferridone is the medicine he wants to do, not the medicine Huarui needs. But one day, Huarui laboratory will need to make its own medicine if it wants to develop and grow. At that time, Yang Rui hopes to be a manager with pharmaceutical experience, not a novice with lofty ideals and talents.

Yang Rui occupied two compartments in the main laboratory and began to design animal experiments.

Wei zhenxue and Wang Xiaoyun are still responsible for the animal experiment. Yang Rui only makes the requirements, looks at the experimental results, and does not directly participate in it.

After all, animal experiments are not in vain after all. Even if the 3R principle is adopted, some experiments are still uncomfortable.

As a graduate student in biology department, Yang Rui has over 100 rats under his command, but that doesn't mean he just likes this job.

On the other hand, it doesn't need Yang Rui to do these things, such as feeding compounds, observing and calculating the results, and finally killing the animals.

Yang Rui is busy enough designing experiments.

In order to pass the FDA or possibly the drug administration of European countries, Yang Rui should first make detailed experimental records, including the preface and follow-up questions, that is, how to think, how to do, and what the results are.More specifically, it's why we think so, why we do it, what we do with it, and the significance of the results.

This complex process can protect the public to the greatest extent, and also restrict the progress of scientific research to the greatest extent.

However, although Yang Rui is very busy, his progress still caught Wei zhenxue and Wang Xiaoyun by surprise.

In just two days, they couldn't carry it. When Yang Rui checked, he found that they didn't even finish one third of the task.

"The reaction time of mice is not our decision." Wang Xiaoyun explained helplessly that she was still busy writing the experiment report. They had more to write than Yang Rui.

Wei zhenxue was so tired that he couldn't turn his head around. He just said, "I haven't slept all day. Boss, add an assistant."

Yang Rui, who works for 110 hours, is also shocked to see Wei zhenxue like this. He asks Wang Xiaoyun, "have you really been up all day and night?"

"I went to bed yesterday. Lao Tu helped me." Wang Xiaoyun is a little embarrassed.

"Where's grace?"

Wang Xiaoyun hesitated and said, "she said she would not accept your order."

"Why?"

"Disorderly life, that's probably what it means." Wang Xiaoyun said: "grace is continuing to follow the regular steps a little bit."

Yang Rui pondered and asked, "how about you? Do you think my experimental design is correct?"

"We've screened out a compound, and from the results, the design works." Wang Xiaoyun doesn't need to praise Yang Rui. What she said is very true.

Yang Rui nodded slightly and said, "then continue to be in the present state... Can't we not add more people?"

"Must add people, at least people stop, the instrument does not stop, so as to speed up the screening."

This sentence moved Yang Rui. The instruments in the laboratory are limited, but it is feasible to use them at different times.

"I'll find two more for you." Yang Rui is not willing to increase the staff of the laboratory too fast. On the one hand, the cost is limited. On the other hand, the busy work of each laboratory in Huarui laboratory is second to none.

After some preparation, the number of compounds decreased rapidly.

If we don't use this method, the three compounds may take hundreds of hours and tens of thousands of yuan to be eliminated.

Within a few days, the number of candidate compounds dropped to an astonishing eight, and grace couldn't help coming to learn from them.

……