Chapter 584

Within a few days, the paper Yang Rui sent to JMC was included. The other party used the telegram to inform the collection first, and then sent the formal letter by mail.

Yang Rui is the corresponding author. Naturally, he came to open the letters and pass them on to the public to stimulate their morale.

Huang Mao praised: "a big magazine is good. If we send a telegram first and then send it by mail, we will feel more at ease here."

Li Wenqiang said: "it's just that there are too many procedures for censorship, which is too troublesome. However, what we published this time is very fast."

"Because it's Yang Rui's name." Wang Xiaoyun tut tut two, way: "Yang Rui now all hang up number in the other side."

"In particular, I wrote a sincere letter." Yang Rui laughed twice and said: "what JMC needs most is the author who contributes a lot. I promise the other party that I will contribute more than three articles in the next month. We should work hard."

"Three contributions? So much? " Zhang Xuetong, who has been squatting in front of the DNA synthesizer for a long time, finds it a bit difficult to complete.

Yang Rui nodded and said, "we are now a laboratory. You can count me, Huang Mao, Tu Xian, Wang Xiaoyun, Wei zhenxue, Li Wenqiang and Duan Bo, and you, Zhang Xuetong. These are the eight full-time researchers. Plus the full-time assistants Wang Lei and Zhou Yuwen, if we work overtime, we can't write three papers. We can't even finish 30 in a year, How can we do that. From today on, each group should finish the project as soon as possible according to the assignment. "

It is not much for a laboratory to complete 30 papers a year. According to Yang Rui's standards when he was a graduate student, the Provincial Key Laboratory in the upper middle class had at least two or three experimental groups, each of which had six or seven experimental dogs and one or three full-time teachers. Experimental dogs write one paper in two years, and full-time teachers write two papers in one year, which is the minimum standard. Experimental dogs who can't write one paper in two years can't graduate. Full time teachers who can't write two papers in one year can't even be counted as those who eat and die. It's normal for full-time teachers with a little level to write seven or eight papers in one year, Thirty SCI level papers are not difficult at all. If not, how can China become a big country of papers.

In fact, the scale of Huarui laboratory in China is at the first-class level. Only a handful of laboratories from Peking University, Tsinghua University or Chinese Academy of Sciences pull out the total amount of instruments over 500000 US dollars.

After 30 years, there is no room for such a laboratory.

Zhang Xuetong used to work in a local university with few resources and slow pace. When he saw Yang Rui's 30 articles, he believed them.

Huang Mao and Tu Xian look at each other and smile bitterly, knowing that Yang Rui is forcing them to work overtime again.

However, it's common to work overtime for research. If you do it slowly, the results will be all of others' own.

A little chat for a while, a few people were Yang Rui command into the experimental state.

Huang Mao collected the experimental reports in recent days and began to write a new paper, a new method to improve the yield of oligonucleotides.

Huang Mao, who has been to the United States, has the highest level of English among several people. Writing English papers is easier than writing Chinese papers.

If it's a top-level paper, Huang Mao will be the main writer and the first author.

Of course, his own contribution is also the contribution of the first author.

Tu Xian, Li Wenqiang and others formed their own groups to share their full-time assistants Wang Lei and Zhou Yuwen. Wang Lei is a little fat man who has met with Li Wenqiang. He and Zhou Yuwen are both young people with low requirements. They are young and strong, and they just work hard in the laboratory.

With the supply of raw materials, Yang Rui began his attempt to replicate DNA.

This is a slightly embarrassing place.

Yang Rui must try from the simplest part, even if he knows it is wrong.

However, correct experiments are always born out of numerous mistakes. Unless they are born with knowledge, they will not succeed in one attempt, and most of them will be regarded as plagiarism.

Such remarkable achievements must be well founded.

Yang Rui prepared a separate experiment, using five to ten cycles method, distributed heating reaction solution, cooled and then added polymerase extension template.

After several cycles, Yang Rui added radioactive labeling. Finally, he precipitated the DNA sample after enzyme digestion, and then ran electrophoresis.

Of course, there is no gel strip behoove.

"Pcr01" failed!

Yang Rui made a record in the experimental book, and then did the pcr02 experiment without hesitation.

In the "pcr02" experiment, Yang Rui pre purified the template and removed the factors that might hinder DNA amplification.Obviously, this is not enough.

Therefore, "pcr02" failed.

Yang Rui, expressionless, recorded the reasons for the failure in the experimental book, analyzed the process and summarized the reasons

For the next week, Yang Rui lived like this.

If there is any advantage, it is Yang Rui's practice in the process of repeated experiments.

Even in the laboratory of later generations, the materials are relatively rich, and there are not so many experiments for experimental dogs.

Even if the realistic conditions are met, psychological pressure will be unbearable.

Just like the experiment of potassium ion channel, Yang Rui didn't participate too much in it. Instead, he gave the experimental work to Huang Mao. Because it's a trial and error experiment, you don't know which one will be successful, but you dare not relax every time, for fear that you can't make an answer because of operational errors, which will lead to failure.

This kind of experiment, psychological pressure is great.

PCR On the contrary, PCR itself is an excellent experimental method, as long as the method is correct, it will not be particularly difficult to operate.

Yang Rui knows where he is wrong, so he has no psychological pressure. He just keeps repeating and accumulating failure experience.

In the eyes of other people in the laboratory, Yang Rui is a bit terrible.

No expression of the experiment, over and over again to do the experiment, do wrong again, do wrong again, and no regret.

This makes Huang Mao and others extremely difficult to understand.

Also very worried.

Therefore, the next week, when the school sent a message to hold a small meeting, Huang Mao didn't refuse on behalf of Yang Rui as before. Instead, he found Yang Rui and informed him, "the school has seen that you haven't been back for many days. Now we have a meeting to see your people. Shall I help you find a car? "

Yang Rui is also a brain to do experiments, as if to go back to graduate school, asked: "what will?"

"Your counselors hold meetings, which are about the final exam, and maybe sports meetings and debates“ Huang Mao was very familiar with Peking University and said, "student organizations such as the Calligraphy Association will also organize activities during this period."

"Why?"

"Because the course is basically over, and there is still a period of time before the final exam, which is just a gap period... In short, let's go to the meeting. We will continue to work on the projects in the laboratory. " Huang Mao is full of persuasion.

"All right." Yang Rui didn't think much and agreed. He hasn't published any key papers on PCR yet. It doesn't matter if he leaves for a while.

However, back on campus, Yang Rui also has a slight maladjustment.

It's not that he doesn't adapt to the campus atmosphere, but the campus environment makes Yang Rui a little uncomfortable.

The laboratory is orderly and controlled, while the campus is free and chaotic

Yang Rui unnaturally went to the ion channel laboratory, did not go in.

Recently, his contact with the ion channel laboratory has been carried out by telephone. It's very smooth, and there's no need for him to go there. However, it gives him a sense of transition.

Yang Rui just stood in front of him, looking at the poplar in a daze.

"Yang Rui, you are back!" The sound of surprise interrupted Yang Rui's meditation.

"Bai Ling?" Yang Rui turns his head slowly.

……