Chapter 101

Because of the particularity of prion, Zhou Wen was very careful when doing experiments.

Biological masks, biological gloves, biochemical protective clothing, fully armed.

Qin Haitao and the four of them just helped, and did not directly contact the vessels containing the virus.

There are four methods to detect prions.

1. Electron microscopy.

2, capillary SDS- gel electrophoresis.

3. Capillary electrophoresis immunoassay of fluorescent labeled peptide chain.

4. Two color intense fluorescence target scanning method.

, among them, the second "gel electrophoresis" is the best and the most direct detection method.

PrPSc was purified by physicochemical methods such as differential centrifugation, ultracentrifugation, sodium n-lauroylsarcosine and PK.

was then performed capillary SDS- electrophoresis on a capillary electrophoresis apparatus.

The prion pathogen PrPSc was identified according to the time and shape of the absorption peak at 214nm.

This method needs less samples and does not need immunologic reagent.

The disadvantage is that we need to use "ultracentrifuge" and "special capillary electrophoresis instrument".

At present, most of the ULTRACENTRIFUGES are purchased from foreign brands, and the price is as high as 500000 RMB.

And prion specific capillary electrophoresis instrument is also very expensive, requiring more than 200000 RMB.

Even if the price is high, there are few opportunities for key use.

General hospitals can't even earn money from purchasing equipment, and only the top three hospitals with money like Jiangzhou hospital will purchase.

Because of this, medical resources in big cities are scarce.

A strange disease in a small city, you can't even test it.

According to the physical and chemical methods, Zhou Wen prepared the reagent step by step carefully, extracted the diluent, and separated the variant prion protein on the ultracentrifuge.

Just upgraded biological immunology, let him more handy.

In this process, Zhou Wen also taught Qin Haitao and Dong Wenying about the points that should be paid attention to in the process of routine prion physical and chemical tests.

"When adding lauroyl, you must remember..."

These experiences, not to mention university professors, even those experts in prion research institutes in China, are not clear about them.

Because the domestic research on viruses, especially prions, is still in its infancy. Only the top foreign research institutes can master these practical experiences.

For example, the Institute of yeast prion, University of Kent, UK.

For a long time, the new discoveries in the field of prion proteins are almost monopolized by several yeast research teams.

Those top research institutes will not easily teach you technology.

Unless you get their offer.

Qin Haitao and Qin Haitao have not realized how lucky they are. They just think that Zhou Wen's explanation is very simple and easy to understand, and even tells them the essentials of his actions.

When Zhou Wen said that, Qin Haitao and Dong Wenying listened attentively.

As for the other two old experimenters, although they seemed to be listening, they actually didn't listen at all.

Because they think that it is impossible to extract prions by Zhou Wen's method, and such teaching is basically on paper.

Zhou Wen has no "electric eye". How can he know these specific experimental variables? Everything is just his guess.

After purification of PrPSc, Zhou Wen injected the supernatant containing prion into Beckman pace5500 for electrophoresis.

"OK ~" after the injection, Zhou Wen was relieved.

"Qin Haitao, you can stay and look after the machine, and others will wash their hands and eat."

After hearing this, everyone went to the sink to wash their hands

After dinner, Zhou Wen didn't dare to go anywhere and went back to the laboratory directly.

The samples obtained by conventional extraction method are too few, so it's really uneasy not to look at them in person

At 7:30 p.m., the electrophoresis experiment was over.

In this experiment, one of the former experimenters thought that he would take a look at the failure of the experiment.

However, the next second, he showed a surprised look on his face, "Hey, look, it seems that prion has been detected..."

On hearing this, several others gathered around.

"Well, that's true."

"Teacher, come on, we have detected the prion."

"Hiss It shouldn't be. How can it be? Is there a mistake... "

Zhou Wen took over the analysis sheet and looked at it carefully, and soon his face showed irresistible joy.

No matter how well the previous experiments went, nothing was 100% until the results came out.For example, failure of capillary electrophoresis instrument can also lead to detection failure.

This will cost all previous efforts.

So sometimes, when you do experiments, luck also takes up a part of the factor.

After sure it is not eye flower, everyone's face shows an indescribable excitement.

To be honest, neither qinhaitao nor Dong Wenying thought prion protein could be extracted by conventional methods.

It is really a very proud thing to extract prion protein by conventional collection method without hope.

After excited, qinhaitao immediately informed Yang Liqun and zhangshuguang.

After hearing the words, the two men rushed over.

"According to the test, this is the prion......"

Yang Liqun and zhangshuguang looked at the clear and white electrophoresis analysis sheet, and their faces were full of surprise, joy, excitement, excitement and incredible looks.

"Is this really detected in the urine?"

"Yes, I have to take a group! The samples were collected by me and Zhou Wen. "

Hearing Dong Wenying's words, Yang Liqun and zhangshuguang were incredible.

Under the condition of routine and non routine detection, prion was extracted, which has different significance.

This is a very important discovery, with high research value.

"You are all good. Although this is only a small step in prion research, it is a big step in prion field in China. "

"Thank you Yang for your praise. The main reason is that Mr. Zhou, if not for his persistence, may have given up at the sight. "

Yang Liqun and zhangshuguang look at Zhou Wen.

Both of them have been very optimistic about the young man, and Zhou Wen is not disappointed. He has shown a very strong talent in biomedical.

And often it brings them some surprises.

This time, for example.

Yesterday they also predicted that it was absolutely impossible to extract prion protein under conventional methods of collection.

Results Zhou Wen "faces" the next day.

But the face is comfortable.

If they can, they want him to keep on with his face, and it's better to bow left and right!

After excitement, Yang Lijin and zhangshuguang went to the computer to analyze and study the sample collection records, hoping to find out some rules from them.

Zhou Wen also admired Yang Lijin and zhangshuguang for their behavior.

That is the spirit of scientific research.

Although no one wants to collect prion protein in normal state, it can not be studied because they do not want.

Only knowing which factors will lead to prion protein excretion through body fluids can we better prevent and control.

The next morning, Yang Lijin reported Zhou Wen's findings to the hospital and asked for the formal establishment of the prion research group under the testing center.

Hospital leaders held a special meeting for this purpose. Would you like to approve the establishment of this research group?

As the First Affiliated People's Hospital, whether it is hardware equipment or talent reserve, it is not too bad, but why can't it compare with other hospitals?

The main reason is that Jiangzhou first hospital is all kinds of access, all kinds of loose, no one to take the hand of "unique life.".

For example, Jinling military general hospital in the province is famous for kidney disease.

For example, Huashan Hospital in Zhonghai is very aggressive in dermatology, infection department and neurology department.

For example, Kyoto University First Hospital, in urology is strong invincible.

And orthopaedics at the Long March Hospital

As for the four tiantuan groups, the level of gods is North Union, South Xiangya, East Qilu and West China. Jiangzhou hospital can not catch up with the horses.

In short, Jiangzhou hospital can compare with other municipal hospitals except Jinling in the province. It is not worth mentioning at all in China.

Because of this, Jiangzhou hospital urgently needs the same medical technology "town field".

Finally, the research team applied for full vote.

Because of Jiangzhou It should be said that there is no prion authority expert in the whole province of Sudong.

As the sponsors of the virus research, Yang Liqun, zhangshuguang and Zhou Wen undoubtedly became the backbone of the research group.

Under the selection of Yang Liqun and zhangshuguang, Zhou Wen became the leader of prion research group of the detection center, and they were two honorary deputy leaders.

The first group members: qinhaitao, Dong Wenying, huangxingzhong, Liushan

.

-

PS: sorry, please

www.novelhold.com , the fastest update of the webnovel!