Chapter 1504

The shotgun method is not the best method for gene sequencing.

Its accuracy is very poor, so another translation name is shotgun.

Don't count on it. It's characteristic of a large area.

In the eyes of the most traditional biologists, this method is not very popular. Because it's counterintuitive, it's also problematic for accuracy.

Sanger sequencing is one base pair one base pair sequencing, completely in accordance with the sequence of genes. The advantage of this is to complete the sequencing of a base pair and complete your map.

The human genome project is based on this. Watson and others dream of using 3 billion US dollars, an average of one dollar per human gene, to make a human genome map.

Of course, they also know that it is not enough to sequence a gene for one dollar because of the current difficulty of sequencing. However, it is just a hoax. Considering the dividend of technological progress, the cost savings and the additional cost in the later stage, it seems that it can be completed.

It just can't be finished - isn't darbeco's cancer plan unfinished? If it's not done, it's not done.

Compared with Sanger sequencing, the shotgun method has more outstanding technical features, but it is also more troublesome.

The shotgun method is to shoot a gene chain into pieces.

It's like a dog's chain. When the gun goes out, it's naturally disassembled into metal rings, and then the atcg of the metal rings is measured.

To this step, it is very simple, can be said to be a shot thing.

The next step is the one that darbeko and others dislike most.

You need to rearrange the metal rings in the original order to make a dog chain.

This step is very difficult, so in history, the shotgun method was originally used for microbial sequencing. Because the genes of microorganisms are simple, they are easy to arrange.

After the human genome is replaced, it's much more difficult to sequence it with a bird shot than a jigsaw puzzle.

Unless——

"We use computers to solve the problem of bird shooting disorder." Standing in Huarui laboratory, Yang Rui said the direction of the experiment without thinking about it.

Huarui laboratory is his private laboratory, all rights are in his hands, and there is no need to worry about anything.

But Huang Mao was a little worried and said, "it costs a lot of money to do the human genome project. Can you earn it back?"

Yang Rui laughed and said, "when did you care about the funds?"

"I'm not afraid your company will go bankrupt." Huang Mao said naturally: "I heard that the bankruptcy law has been implemented, and insolvent companies will go bankrupt."

In the first two years, several state-owned enterprises were forced to close down in China, and they made a lot of reports. They were the only enterprises that closed down in the 1980s.

"We're far from being able to pay our debts. You can take a hundred heart."

"I want to rest assured that you can't keep up with the follow-up funds." What Huang Mao said is very casual.

"There will always be funds when there is a need. Start to do it first." What Yang Rui thinks about is that he has hundreds of siheyuan apartments, as well as more than 20 buildings in a commercial community he has just bought, and rooms full of antique tea and stamps. He always feels that he is rich.

He is, of course, very rich by the standards of 30 years later.

But in the late 1980s, although the houses in Beijing were still out of reach for ordinary people, they were not enough for scientific research. Even if we sell the commodity community we just bought, that is, the daily expenses of Huarui Laboratory for several years, we don't want to fill the bottomless hole like human genome project.

If you think so, thanks to the soaring house prices. Later generations of research institutes actually have many dormitories for allocation, and the researchers' property is mainly concentrated in their own real estate. Their asset value-added speed really exceeds the value-added speed of technology development... In theory, if some large research institutes sell all their land, they may be able to develop the human genome project themselves

Yang Rui's idea, Huang Mao couldn't know about it. He listened to Yang Rui's words, shook his head slightly and said, "it's OK to listen to you, but you have to pay attention. I don't see any possibility of giving back funds for the pre research of human genome project."

Pre research is pure basic science, and there are very few funds to be given back.

If it wasn't for the later Sierra genomics company, to be honest, Yang Rui can't be sure that the technology of shotgun method will sell for money.

As a matter of fact, the celalay gene company has not really been successful. Its leader, frantic Venter, has cheated us $300 million to develop the shotgun method and apply it to the human genome project, and has tried to patent the human gene. If he succeeds, any future targeted drug may be exorbitant.The Sierra Leone company will also be rich.

Unfortunately, comrade Venter did not succeed.

So, to speak of it, the human genome project really has no history of making money.

The pharmaceutical companies that made money later did not give the human genome project team any money in return.

But just because Americans can't make money doesn't mean that Chinese people can't make money.

At this time, the benefits of independence are reflected.

As for Yang's position, such as the cutting-edge technology that involves biology, and the absence of ready to use laws, the formulation of the new laws and regulations has the final say.

GMP The committee is of the same nature.

If you don't let a top scientist like Yang Rui judge, do you want a liberal arts student to guess?

A lot of people think that human cloning is actually copying a person.

In fact, the only difference between human cloning and IVF lies in the genetic level. We still have to conceive in October, give birth naturally or have a caesarean section, and then receive the same education as normal children. If we want to get the same Hitler or Einstein, we have to see if our parents have the ability!

To be qualified to participate in law making is the hidden power of top scientists.

From the perspective of one's needs, why are so many people willing to immerse themselves in the painful and boring scientific world? Some of them really like the scientific world, while others take it as a springboard to enter the power class.

For example, some famous economists in the 1980s and 1990s played a greater role than many ministers, and really changed China.

It's hard for people who are suffering in the stock market, happy for the stock market and depressed for the stock market to imagine that the stock market has depended on the reports of several people for as long as 20 years.

Similarly, people suffering from high blood pressure, rheumatism, hyperosteogeny and tracheitis can live a few more years and have a much easier life if a few top biologists are willing to turn around, just like those diseases that have been paid attention to, such as diabetes and AIDS, The living conditions in the 1990s, the 1990s and the 1980s are quite different.

**The life of patients with dysfunction is completely changed by the blue pill.

Before the blue pill, a lot of men's difficult words were true and could not be saved. After the blue pill appeared, the most difficult place for most men, that is, the hairline was gradually moving, so they could not hide.

"The problem of funds cannot be ignored. Well, if you're really worried, we'll open another group to take antihypertensive drugs. " Yang Rui is undoubtedly ready to pull out Viagra.

The human genome project is a costly project. Despite Professor Cai's promise to help find money, Yang Rui himself can't relax.

As he said, the allocation is determined by the proportion of funds. He still needs some efforts to ensure the control.

Find something that costs less and works faster. Naturally, you will think of Viagra.

Of course, the main ingredient of Viagra today is still used as a antihypertensive drug.

Huang Mao did not doubt that there was him, only said: "at the same time to open a group of antihypertensive drugs to do, will be busy."

Yang Rui has made many kinds of new drugs, and every time he makes a lot of money. It's not surprising that he makes drugs in order to make money. He only worries that the scale of Huarui can't support two projects.

"I'm more interested in the current discussion of nitric oxide, and I'm going to buy a compound to do it," Yang said with a shrug

"Why, shall we buy compounds?"

"Well, I'll get someone to buy it. You don't have to worry."

Huang Mao said with a bitter smile: "how can I not worry? In the past, we made active compounds and sold them to pharmaceutical companies for clinical trials? We don't have clinical experience... "

"There's always a first time." Yang ruicai atmosphere rough way: "we have money, rest assured."

Huang Mao is even more worried, for fear that Yang Rui will lose all his money and buy back the compound that has no egg use.