Chapter 1471

Yang Rui took out a Montblanc from the drawer. Before he wrote, he patted his head and said, "go and make a copy first, so that I won't be scratched."

"It's all right. You can row as you like." Gu Qiang's attitude is extremely clever.

"It's better to make a copy and keep a copy for later comparison." Yang Rui insisted.

Gu Qiang had no choice but to copy according to his words. Looking at the hundreds of pages of experimental design, he was reluctant to part with it. Not reluctant to write his experimental design, but reluctant to copy paper and ink.

Today's copiers are made in Japan, the price of the machine is expensive, not to mention, the consumables are extremely expensive. When Yang Rui was working on examination papers in school, he always organized mimeograph.

Ordinary materials, we also use the method of copying, try not to waste copy paper and other consumables.

Now, of course, I can't wait to copy.

Gu Qiang printed a copy of the experimental design he wrote. When he returned it to Yang Rui, he said, "I'll find someone to copy these things in the future. It's too expensive to print. The paper costs a few cents."

"It's not expensive to find someone?"

"Ten yuan a month is enough for work study students." Gu Qiang naturally said.

10 It's a lot of yuan. Now in many rural areas, the annual cash income of a family is less than 120 yuan.

If students eat in the canteen, 10 yuan can ensure a full stomach, but also bring some meat.

Yang Rui ignored this. He looked down at the experimental design of Gu Qiang's printed version and took off his pen cap. He said, "if you can save office expenses, you need to save a little. Let director Xu worry about it. Don't waste your time on it. The laboratory wants you to produce, not to save."

"Saving is output." Gu Qiang whispered.

"If you can complete the subject of in vitro culture in two months, it is the real output." Yang Rui's red pen crossed over Gu Qiang's experimental design, and three circles were circled in the first line.

Gu Qiang was not only shocked by the red mark, but also surprised by what Yang Rui said. He could not help repeating: "two months?"

"The progress of Wang Liangcai's research group will also be accelerated." Yang Rui doesn't say why, everything is in silence.

Gu Qiang's mouth twitches twice, knowing that Yang Rui must have been involved in Wang Liangcai's research.

Think about it, it is impossible for Yang Rui not to intervene.

Since the beginning of the cloning project, Wang Liangcai and Gu Qiang are busy competing and doing experiments, and Yang Rui has never stopped thinking.

Now, he will speak out the results of his consideration, which will naturally help to improve the progress of the project.

However, in two months' time, the subject of in vitro culture will be done from scratch, but Gu Qiang is not confident.

"I thought there would be more time." Gu Qiang's cowardly way.

With a smile, Yang Rui picked up his pen again and began to draw a red circle.

On the first page alone, he drew a dozen red circles, almost beyond recognition.

Gu Qiang secretly took a breath, then lowered his head and went to see where Yang Rui had drawn.

The word "IVF embryo" is circled and crossed, and the oxygen consumption next to it is circled without any other modification.

Later, Gu Qiang saw the circle drawn by Yang Rui for the word "densification" and the circle drawn for "culture medium".

Gu Qiang finally knows why Yang Rui asked himself to make a copy.

This way, the whole experimental design will be changed beyond recognition.

If you don't leave a bottom plate, you really don't know the original details.

"I hope you can focus on the embryos after blastocyst stage. But you need to pay attention to more than that. " Seeing that Gu Qiang was watching, Yang Rui didn't seem to have been hit too much, so he explained in passing while drawing a circle and a fork, saying: "for the culture of cloned embryos, you want to refer to the culture system of in vitro fertilization embryos, but I don't agree with it."

Yang Rui pointed his pen on the red fork and said: "of course, the judgment of the front-line researchers should be the first standard. But from my personal understanding, if we adopt the original system without analysis, the result is probably not to save time, but to waste energy."

"Do you think cloned embryos will be different from normal embryos?" Gu Qiang was surprised.

Regardless of theology, scientists have a clear understanding of embryos and other reproductive bodies. They believe that cloned embryos should be the same as normal embryos. Otherwise, how can cloning cause so much controversy.Therefore, in the process of in vitro culture, Gu Qiang naturally adopted the same technical scheme as "test tube baby" and "test tube cow".

Yang Rui put the pen down for a while and said, "I don't think cloned embryos are different from normal embryos in essence, but of course they are different. First of all, the cloned embryos we got were developed from eggs with few cytoplasm, which was congenital defect. Secondly, oocytes are soaked in a variety of operating solutions. For example, as we know at present, sucrose solution will have adverse effects... "

Yang Rui's voice was steady, and he said: "in a word, cloned embryos are abnormal embryos. If you use normal embryo culture methods, you are likely to have problems."

In fact, Yang Rui is sure that it will go wrong.

This is also a difficulty in vitro culture.

More problems will be accompanied by poor development of oocytes.

In short, in order to change the nucleus of an egg cell, the operation of scientists has caused great damage to it, and in vitro culture is to make up for this damage.

Take chestnuts for example. If we regard 5 jin as the minimum standard weight of a newborn, Newton, who weighed 2.7 at birth, will not survive easily. The modified cloned oocytes, about equal to a child weighing 0.5 Jin, are unlikely to survive in natural conditions.

In order to make it develop normally, it is no longer a question of whether to feed Sanlu milk or not and whether to buy imported milk powder.

Of course, if you can't afford imported reagents, the oocyte won't survive.

Shocked by Yang Rui's detailed explanation, Gu Qiang asked in a low voice, "have you ever done in vitro culture before?"

"We did some preparatory research when we did embryo transfer for cattle." Yang ruidao.

Gu Qiang is full of doubts. Although the word "embryo" is also used in bovine embryo transfer, it is different from the problem we are facing now in order of magnitude.

If we don't talk about it, we have to spend millions of dollars on the in vitro culture of cloned sheep. The remaining resources of Guqiang group had to be transferred.

What about bovine embryo transfer? Even with the implicit investment of China animal husbandry and other companies, the total amount is not so much.

Gu Qiang licked his lips and asked, "when you do bovine embryo transfer, you want to do cloning."

Yang Rui glanced at Gu Qiang with a smile, but did not answer. He pointed out the experimental design with a pen and said, "next, there are the details of the experiment. I have some ideas for your reference."

"You said Gu Qiang has a positive attitude.

"From the data, adding various additives to the basic nutrient solution should also be able to improve the development of cloned embryos." Yang Rui did not elaborate on any information, and said: "we start from the cell embryo culture, we have to face the blocking of embryonic development."

The blocking of embryonic development refers to the phenomenon that the embryo stops developing when it reaches the four cell stage. This is mainly related to the activation of embryonic genome.

Now there is no genome project. To break this block, we have to do a lot of various attempts.

Yang Rui did not elaborate. He just drew two circles in Gu Qiang's experimental design and said: "I agree with the two schemes of adding oviduct fluid to the culture medium and co culturing with oviduct epithelial cells. You can try it first. "

"All right." Gu Qiang nodded.

"Culture medium is also a key point. You have to keep a complete record of the experiment. " Yang Rui specially reminds us.

A clear formula is very important for the culture medium.

Of course, today's scientists haven't realized this, because no one has started cloning.

In later generations, if we say which part of cloning is the most complex and the most threshold part, it is not the technology of egg stripping, but the modulation of embryo culture medium.

Yang Rui thought about it and decided to help Gu Qiang reduce his burden. He said: "from my personal feeling, we have used fetal bovine serum, a promising additive for culture medium. Epidermal growth factor, this is a new thing, we should try it. Insulin, growth factor, antioxidants, anthocyanins, I think all of them can be considered. In addition, oxygen is also important. "

"All right." Gu Qiang simply recorded it, but he didn't have any clue for the time being.