Chapter 1463

From the perspective of Wang Liangcai, Yang Rui's operation is difficult to highlight.

He is a student of Tong's family. He studied genetics as an undergraduate and trained with scholars from the Institute of Aquatic Sciences of the Chinese Academy of Sciences for many years. After that, he taught and researched in the University of science and technology and played in various genetics projects. Wang Liangcai is a world-class egg peeling expert, far superior to the so-called egg peeling expert of Peking University.

However, Wang Liangcai was eager to write down every action of Yang Rui's operation.

In just a few minutes, which is almost equivalent to the time spent waking up from watching Wong Kar Wai's movie in the cinema, Yang Rui has basically completed the operation of de nucleation.

It's not difficult to remove the core, especially for the researchers from the Institute of fisheries.

But Yang Rui, who failed only once, successfully completed the denuclearization operation, which is really amazing.

In other words, Yang Rui's operation method is not worth mentioning, but he used this operation to easily complete the de nuclear operation, which is the reason why Wang Liangcai paid attention to.

There are two enucleation operations in cell cloning.

The first enucleation is to take out the nucleus of ovine oocyte. Because the nucleus is not used in the later stage, the first key point of enucleation is to suck out the cytoplasm of the cell as little as possible, and at the same time, to suck the nucleus clean.

The second enucleation is for ordinary cells.

Cloning sheep uses the mammary gland cells of sheep. At this time, the key point of operation is to completely suck out the cytoplasm of mammary gland cells of sheep. As for the mammary gland cells themselves, because they are also discarded in the later stage, it doesn't matter.

Generally speaking, the two steps require refinement, and there are few shortcomings that can be tolerated. The best state is to completely separate the nucleus from the cell itself.

Otherwise, if the nucleus left by the oocyte participates in the development in the later stage, it is hard to say whose sheep was born.

If it's used for cloning, what you want to clone is dolly, but dolly carries a beautiful genetic gene. This kind of cloning is not clear.

It's not a blockbuster clone.

From the very beginning, Wang Liangcai was very clear about the purpose of this part of the operation. However, in order to drain the nucleus of the oocyte, he had to absorb more cytoplasm. As a result, the remaining cytoplasm was not enough to develop, so that he declared failure.

In fact, the same is true of Gu Qiang's operation. In this step, the cytoplasm is too easy to be sucked out.

Instead, it's the second step. It doesn't matter how much cytoplasm is lost, it only requires the integrity of the nucleus, and the technical requirements for the experimenters are much lower.

Yang Rui's newly developed technology is different.

Although the goal of the operation has not changed, it is still to suck out all the nuclei and the cytoplasm as little as possible.

However, Yang Rui's operation lowered the requirements. Instead of aspirating the nucleus completely, he deliberately aspirated the nucleus and then aspirated the fragments out a little bit.

Complete aspiration of the nucleus is able to achieve the requirements, but it is too difficult.

Moreover, the nucleus is not a hard solid. It is more like a balloon swimming in a viscous liquid. If it is completely sucked out, it will inevitably increase the suction and suck out more cytoplasm. In addition, when the balloon comes out, it will inevitably bring out more cytoplasm. Sometimes, it will bring out more cytoplasm, which will directly declare the failure of the experiment, Sometimes bring out less, and there is no small element of luck.

If it is broken, it will cause more trouble.

Think of a balloon broken in the liquid, the gas inside the balloon also floated out, in the liquid to form a bubble, one by one to suck out, that's how troublesome.

However, trouble is troublesome, but it is a troublesome work that can be completed. In addition to careful and tedious operation, it is much easier to suck debris than the whole balloon.

Sometimes, if the aspiration needle is well controlled, it doesn't even need to suck a lot of debris. Three or five times of negative pressure suction will complete the task.

After Wang Liangcai discovered this, he could not walk any more.

After that, Wang Liangcai found that suction crushing is only the smallest of the new technologies developed by Yang Rui.

Using the spindle to control the amount of absorption is the most powerful place, it can be said to be exquisite.

As the name suggests, the nuclear aspiration needle is used to absorb the nucleus. How small is its tip? Basically, you don't have to be able to see clearly under an ordinary multiple microscope.Therefore, when operating the needle, the slight vibration will make it swing violently.

You can imagine the feeling of holding a rope in your hand when you are piercing a needle. The cell is much smaller than the eye of a needle. The hand vibrates a lot, let alone sucks the nucleus. It's normal to puncture the cell.

Yang Rui's level is weaker than Wang Liangcai's, which is also a certain level of experimenter. He still accidentally punctured an oocyte, which can measure the difficulty.

However, when the needle touched the spindle, the tremor disappeared naturally.

It's like holding a long stick in your hand, the head of the stick will vibrate, but when you poke the stick on the wall, the head will not vibrate.

In addition to controlling the jitter of the core aspiration needle and increasing the accuracy, another advantage is controlling the front and back jitter of the core aspiration needle.

That is to say, when the needle pokes into the spindle, it is like adding a fault-tolerant device out of thin air, which makes the operation easier.

It's easy to say, but it's not easy to think of using it.

The most difficult part is that you can find the correct location of the stamp.

Spindle is also a very important part of the cell. It can't be poked if you want to. It is naturally tough, but some parts are too tough to absorb, and some parts are unexpectedly fragile, so they are easy to be punctured.

The remaining spindles, which can be poked, had better be in the better operating position.

Wang Liangcai looked at Yang Rui's action, and he had a thousand twists and turns in his mind. He wanted to master the new method immediately and try it later.

At this point, Yang Rui can not meet the requirements of Wang Liangcai.

What he did was slow, and he only tried a new method several times. It was clumsy and made people want to jump. It was like a child who had just learned how to shoot the ball to make a three-step layup. When he didn't break the rules, he was ugly to death, not to mention that he broke the rules more than he walked.

"Professor Yang, I understand a little bit, otherwise, I'll try to operate it?" Wang Liangcai said in a low voice.

Yang Rui raised eyelid, way: "how, feel my operation is not good?"

Wang Liang just clear cough two, disobedient way: "which can, you operate no problem."

"I also think, just a moment, I'll finish this check." Yang Rui really thinks that he has done a good job, which is not as good as Wang Liangcai's operation. Compared with the previous operation, he has made progress.

Naturally, he ignored the difficulty gap between old technology and new technology.

Wang Liangcai can't be anxious, and can't urge, can only obediently look at.

If he had given Wang Liangcai two kilos of bear nuclei, he would have jumped up to tell the truth. The same conditions apply to other researchers in the laboratory.

As time went on, Su fan, Gu Qiang, Tian Bing and others gathered silently in front of the experimental platform.

How about Yang Rui's experimental ability, we all have some number in mind.

And how long can an oocyte endure the devastation, we all have some number in mind.

Normally, if Yang Rui is given an oocyte, he won't be able to play for two hours without puncturing it.

After a long time, the progress of the experiment will be deeper.

Think about Gu Qiang's luck before he can get the injection, and Wang Liangcai sleeps in the laboratory for several weeks before he can barely catch up with the progress... Yang Rui can't just take Gu Qiang's luck and do it for a few hours at a time.

The more researchers they pay attention to, the more curious other researchers become.

In the afternoon, Yang Rui's experimental platform was surrounded by people.

Yang Rui felt nothing.

Unlike other people's boring experiments, he is trying new methods, just like playing new games. At the beginning, he can't stop.

Goo Doo.

Somebody's stomach, it's ringing.

Yang Rui raised his head blankly, rubbed his sour neck, and then noticed all kinds of strange eyes around him, as well as the moving heads.

"Hey, don't be crowded. Isn't it hot?" Yang Rui reluctantly drove out the people: "pay attention, don't pollute the experimental platform."

We quickly stepped back two steps to make room.

"It's almost eight o'clock. If you don't have a meal, go and have a meal. I'll be here for several hours." Yang Rui felt his stomach and decided to wait for food. His stomach could wait, but his cells could not.

Everyone nodded and said to each other, "Lao Tian, you have a bad stomach. Hurry to eat."

"Xiao Lei, don't hang on. Go to dinner.""Su fan, you have your favorite braised pork today. You can do it fast and slow."

"Then why don't you go." Su fan turned his lips and didn't care about other people's persuasion.

In fact, no one wants to go at all.

What a rare opportunity to see a demonstration of new technology. Yang Rui, a Nobel laureate, was demonstrating it. Later, he said, "when I saw Professor Yang do the enucleation of egg cells", his style naturally rose.

Yang Rui didn't say much. He was just demonstrating technology. He couldn't drive people away.

He felt his stomach and continued to work, and the others gathered together as usual, watching and discussing in a low voice.

There are always different levels in a laboratory, especially in the face of a specific topic, some people have a thorough grasp of it, and some people are not very good at it.

At this time, the focus of attention is different.

The so-called layman watching the scene and the expert looking at the door are the same in the same laboratory.

Even if you just look at everyone's notebooks, Wang Liangcai recorded more than others.