Chapter 1460

"Professor Yang, now we want to do a batch of comparative experiments to improve the success rate." Su fan and Wang Liangcai prepared for several days before they went to find Yang Rui.

Although working in a laboratory, the person in charge of the laboratory is not easy to disturb. There is no dry goods in his stomach, so he is easily bullied.

Yang Rui doesn't pretend to be a know it all when he is in the laboratory.

Even if he started the sheep cloning project, he would not do every experiment personally. In fact, for such a large-scale project, his task is to urge the completion of key points. As for the small parts, it's not worth the effort.

Therefore, Yang Rui did not pay any attention to the problems of Su fan and Wang Liangcai. He nodded calmly and said, "very good."

Originally, the only way is to do comparative experiments. If Yang ruijiao is still needed, the team will be too weak.

Su fan had been familiar with Yang Rui's character for a long time. He said with a smile, "Professor Yang, we have some ideas on comparative experiments. I'd like you to have a look."

"Oh? Which ones? " Yang Rui is willing to do this kind of specific problem.

"The first is the pretreatment solution. Gu Qiang uses 3% sucrose, which seems to work very well, but he can't say why. Considering that sucrose solution may cause certain chemical damage to oocytes, we think it's necessary to investigate the proportion of sucrose solution. " Su fan put forward the first question tentatively.

Yang Rui nodded and said, "that's right. What's the problem?"

He has automatically and consciously put himself in the perspective of a problem solver.

As soon as Su fan heard the play, he said: "I wonder if we can ask the laboratory to organize a team, or send us more funds and manpower to do this comparative experiment."

"Well..." Yang Rui hesitated to add more resources to Su fan and Wang Liangcai's group.

Their progress is not slow, but their style of playing steadily is not recognized by Yang Rui.

According to the current domestic economic situation, even Yang Rui's laboratory can't get the international average level of funds. It can only be said that the low price level reduces the operating expenses of the laboratory. However, many experiments point out that foreign exchange is used, and the funds are always tight. If every problem is solved with resources, it will be weak in the later stage.

"Just a pretreatment solution, start a research group. It's a bit of a fuss." Yang Rui pondered for a moment and expressed his opposition.

It seems to be a good topic to do some research in local universities. Many scholars probably don't mind spending a year or two here.

But from Yang Rui's point of view, there are too many steps to clone sheep. The pretreatment solution is just a small point, which can't be any smaller. How can we study it thoroughly.

Su fan tried his best to reduce the expenses, saying: "in terms of pretreatment solution, an additional 100000 to 200000 yuan should be almost enough."

If you save some words, 200000 yuan can still be relatively clear about the pretreatment of the operating fluid research, 100000 yuan is a joke.

Yang Rui shook his head.

20 Even though the total amount of funds he received was eight figures, all the projects and research groups spent money at a running price. It was not a good example to spend 200000 yuan on just a pretreatment fluid.

In the final analysis, it is the right way to save funds and expenses for domestic projects.

"I think you can adjust your development direction." Yang Rui thought about it for a moment and said, "your problem is that you are worried about the chemical damage to oocytes caused by sucrose solution, so you want to choose a solvent with as little damage as possible, right?"

"Yes. We hope to increase the success rate as much as possible. It is difficult to estimate the chemical damage caused by sucrose solution. We are worried about the impact on the later success rate. " Wang Liangcai quickly explained to Yang Rui.

"It's not hard to understand your worries in this respect, but it's hard to verify the degree of chemical damage," Yang said

"Yes..." Wang Liangcai was helpless.

"I think it should be considered in two ways." Yang Rui spoke slowly.

In pointing out the direction of the experiment, Yang Rui has always been not stingy, nor too much scruples.

Seeing the direction from the chaos and driving in the fog are what scholars do.

If you do well, you will only get praise, not doubt.

Wang Liangcai also suddenly came to the spirit, listening to Yang Rui with breath holding gaze.

Yang Rui considered his tone and said, "first, let's talk about the pretreatment of the operating fluid. You can not only consider the reduction of chemical damage, but also the protection of oocytes."Wang Liangcai and Su fan looked at each other and didn't say a word. This is one of the obvious strategies, but the strategy can't be realized.

Yang Rui tapped the table twice with his fingers and decided to speak more clearly. He said, "if I were to do this experiment, I would definitely not use pure sucrose solution. I'll think that it's a good choice to give oocytes a more adaptive environment, for example, fetal bovine serum

"Fetal bovine serum..." Wang Liangcai didn't expect that Yang Ruizhen could say something. Moreover, it sounds reasonable.

In the genetics laboratory, serum is also a common reagent, and fetal bovine serum is not strange to people. However, Wang Liangcai and Su fan, who are full of sucrose solution, would not have thought of it if it had not been proposed by Yang Rui.

In fact, the whole laboratory, including Guqiang group, did not try to add fetal bovine serum into the operating fluid.

"Let's try." Wang Liangcai was a little excited.

Only judging from experience, adding fetal bovine serum is also a reliable choice.

"What's the second point, chief?" Su fan also thinks it's reliable. He's been doing cell cloning for a long time, and he's also used fetal bovine serum. Now in retrospect, all the indicators fit well.

Yang Rui said with a smile, "second, I think you should change your thinking."

"Yes."

"Our goal is to clone sheep, en, clonal sheep... In a word, this project is not a commercialized project for the time being, so we only need to make a cloned sheep in the end, with unlimited success rate and low cost performance."

Yang Rui seems to be very relaxed, as long as a cloned sheep or something, listening to Wang Liangcai and Su fan's ears, it is simply helpless.

But what Yang Rui said is very reasonable. If there are sufficient funds and sufficient staff, it is naturally the most simple and feasible mode to push from bottom to top.

However, it is basically impossible to have sufficient funds and manpower.

There are few scientific research projects that allow scholars to wantonly consume resources.

From Yang Rui's point of view, sheep cloning is a bottomless project.

Even in another 30 or 40 years, people will have little understanding of the key points in cloning.

In history, the Scots did more than 600 groups of experiments to make dolly a cloned sheep. If their previous research is very sufficient, even for insurance measures, two or three cloned sheep may eventually be born.

It can only be said that Dolly, the cloned sheep, also has a certain element of luck.

Before it, there were several abortive cloned sheep, which wasted a lot of time and energy.

If Yang Rui does not want to spend the next 10 years on the same project, it is imperative to adopt a more economical way.

Studying every key point thoroughly is neither a right choice nor a possibility, and it is even more unnecessary.

As long as Yang Rui's laboratory can complete the project of sheep cloning, and there is no clear key point to study the previous loopholes, naturally someone will come to check and fill the gaps.

So many scholars from local universities, young people who have just started their career, and poor people's laboratories in the third world, have done this kind of work all their lives. They are not tired of it, and they have to give others a chance.

Su fan and Wang Liangcai also understood.

Yang Rui obviously does not agree with their step-by-step mode. Wang Liangcai can't help but feel a little anxious and says, "Professor Yang, director Yang, sheep cloning is a series of projects. If the success rate in the early stage can't be guaranteed, he doesn't even have the qualification to enter the later stage."

"Didn't Gu Qiang do it?"

"We can't learn Gu Qiang's method. He himself knows what it is and doesn't know why it is. " Wang Liangcai sighed.

Yang Rui calm way: "then know its natural, don't know why its natural good."

"Ah?"

"We are in the same laboratory, the same project group, divided into different research groups, just to try different ways. Since Gu Qiang has got one, let's make use of it. Why spend his experience on opening up a parallel road? My suggestion is that, until the part of verification, you should only carry out the work of checking and supplementing the defects. Starting from verification, you should continue to work hard. "

Wang Liangcai was stunned, but he had the feeling of enlightening.

"You're right. There's no need to worry about why to use 5% concentration. 5% concentration is easy to use." Wang Liangcai can't help knocking on his head.

Has anyone studied an experiment thoroughly?

Of course, there are, but those who do this step are usually studied from the back to the front, that is, later generations study the achievements of predecessors.Scholars who are really doing pioneering research and standing at the forefront of science are like strangers exploring in the primitive rainforest. Who has the energy and time to explore the species and characteristics of each tree? Who can figure out the life under every inch of soil, or observe the movement in every crown?

Doing research is like driving in a fog. You can't stop. You're waiting to be hit by a car. You don't expect to have how far visibility, can see the road at the foot, don't fall down on a good.

Of course, Gu Qiang's experimental report lacks detailed analysis, but there is one thing that is OK. At least he has proved that the road is open.

"There should be success rate, but we don't need to pursue the limit. We should solve the problem of whether there is one or not first." Yang Rui is equal to Wang Liangcai and Su fan's original idea to overthrow, but they are not unhappy.

Listening to valuable suggestions is the basic attitude of scientific research.

It's useless to make a strong order with cells, and it's even more troublesome to make trouble with funds.

Wang Liangcai thought for himself. After a moment, he said, "in this case, let's repeat Gu Qiang's experiment first, and then pick out the parts with obviously low success rate for in-depth study."

"In this case, Gu Qiang will lead for some time." Su fan is slightly unwilling.

Wang Liangcai took a look at Su fan and said, "fast first is not fast. Sheep cloning is a big project. It's just like long-distance running. We'll follow the strategy."

Compared with Su fan, Wang Liangcai's character is obviously more stable, and his method is also the best way to make use of their advantages.

Moreover, compared with the silly method of building foundation, the modified scheme also takes into account the success rate and speed.

Most importantly, Gu Qiang's advantage has indirectly become the advantage of the two.

Yang Rui also nodded secretly. In this way, the efficiency of the laboratory can be greatly increased.

No matter how lucky Gu Qiang is, he doesn't really have a direct answer. From time to time, he always needs Yang Rui to correct his direction. Most importantly, due to the lack of theoretical reserves, Gu Qiang's ability to expand scientific research is very weak.

Gu Qiang is not good at such things as backtracking experiments, finding out the defects, even increasing the success rate and improving the stability of experiments.

But in such a long project line of sheep cloning, these capabilities are indispensable.

On the contrary, Wang Liangcai is not a scholar who is good at making surprises, but a stable long-distance runner. Put down a little bit of insignificant face, what Wang Liangcai got was the chance to strengthen the fast running.

"Professor Yang, we also want to apply for more flight hours, especially at night." Wang Liangcai paused and said, "can you give us all the time after midnight?"

"If you apply for the night time, you have to come every night." Yang Rui asked, "can your body stand it?"

"I can take a two-hour nap at noon and another four hours after dinner. In addition, I can take a two-hour nap the next morning. No problem." Wang Liangcai said it was easy, but all three of them knew how cruel it was to spread out the whole sleeping time.

"Don't you need some... Personal time?" Yang Rui has seen people who work hard and often ask the experimenters to work hard. However, it is rare to ask them to work extra long hours.

Wang Liangcai grinned and said, "my family is not here. I want to do something in my private time."

Yang Rui was speechless, shrugged and said: "well, since your family is not in Beijing, you can eat a small stove in the canteen. I'll ask a nutritionist to make sure you have good taste and enough nutrition. By the way, a while ago, I asked for a masseuse, a national level masseuse. When they arrived, they assigned you an hour every day. "

Su fan's envious saliva is about to flow down, but Wang Liangcai himself is at a loss.