Chapter 1236

"Director Yang, what's wrong?" Gu Qiang scratched his head.

"Just because it's so appropriate." Yang Rui muttered to himself: "you are in the presence of detergent conditions, the formation of complex?"

"Yes, the args complex you named, I have tested, can effectively couple in the phospholipid bilayer."

Yang Rui looked up at Gu Qiang and then asked, "is there no decline in coupling efficiency? I mean, in the presence of detergent. "

In the first stage, detergents were also used to treat GPCR. However, different from the second stage, in order to ensure the "purity" of GPCR, detergents are always required in the second stage. This is a higher requirement. The most important thing is that once there are detergents, The coupling efficiency of G protein coupled receptor will be greatly reduced, and it will drop to the point of outrage.

There are two ways to solve this problem, one is to complete a series of experiments before the coupling efficiency drops to unacceptable, the other is to solve the problem of coupling efficiency decline, or at least extend the time of high efficiency.

Yang Rui is coming. Gu Qiang's experimental records are searched.

Gu Qiang's experimental record is only a short page.

Gu Qiang's answer is more simple: "there was a decline in the initial stage. After I changed the solution, the problem of the decline in coupling efficiency was solved."

Yang Rui's voice was about to tremble. He pointed to Gu Qiang's experimental record and said, "you changed the solution of lauryl maltoside."

"Yes. However, it was later found that the complex thus formed was unstable. "

"Yes, it must be unstable."

Gu Qiang nodded and said: "then I remembered the experiment I had done before. When I encountered similar situations, I used enzymes to solve them. I added adenosine triphosphate diphosphate, which just hydrolyzed GDP on the complex to avoid interaction with GS."

"Yes, it just hydrolyzed." Yang Rui felt speechless.

Gu Qiang felt guilty and said, "what's wrong? I did it all according to your plan, and I didn't get any details. "

"The problem is that there is no problem anywhere." Yang Rui stared at Gu Qiang for a long time and said, "how can you make the plan smoothly?"

"Isn't shun Dangdang good?" Gu Qiang has some rebellious character, but he has been tamed by Yang Rui for a long time, but he is a little uneasy at this time.

Yang Rui recalled Gu Qiang's counter intuitive "one pot stirring" technology development and his precise experimental skills, and suddenly asked, "have you brushed your circle of friends?"

Gu Qiang Leng for a moment, said: "no friends play with me."

"All right." Yang Rui sighed and said, "Congratulations, another top-level paper has come out of your hands."

"Do it?" Gu Qiang was stunned.

"Yes, it is." Yang Rui confirmed.

"Did you really do it?"

"I did it."

"No way. It's only a few days." Gu Qiang jump feet excited, the whole person's cheek swelling red.

Yang Rui nodded with approval and said, "I don't think it's possible. It's only a few days."

"Is the second stage complete?"

"This is the crystal structure of the complex?"

"The crystal structure is made?"

Influenced by Gu Qiang's exultation, a group of researchers gathered around.

Like a tour guide, Yang Rui told a passionate story flatly, saying: "although it still needs some mathematical calculation and verification, the crystal structure is almost done."

A group of people looked at each other and finally came to a question: "how can it be?"

Yang Rui sighed with empathy. He didn't know how to explain it.

To say that this story is destined to be a wonderful anecdote, Yang Rui is also inseparable from it. It is he who gave the secret book of customs clearance and the whole set of plans.

Yang Rui did this in order to improve the speed and complete the second stage of research as soon as possible. Given a full set of solutions, researchers will be able to make targeted modifications and tests.

However, given a full set of programs, it took someone a few days to complete the program, which is the most incredible place.

Let alone the Nobel prize level scientific research experiment, which is the junior high school experiment, the teacher still gives a full set of experimental programs, can all the students make the results?Find a middle school chemistry teacher to answer this question, he can say a hundred strange experimental results, are not real experimental results.

How many students can make the results of a slightly more difficult University experiment? One of the three, even if the students are studying hard.

In the weaker universities, it's very common to have a class of organic chemistry experiment in which none of the results are achieved.

g The crystal structure of protein coupled receptor is difficult to experiment, not to mention. The key points are to use the correct detergent and control the relatively appropriate concentration first. The requirements are broad and easy to achieve, but this is not the only point that can be achieved by pulling an experimental dog.

Next, the complex crystal of GPCR was formed at appropriate concentration.

There are even fewer researchers who can make this step. If it is regarded as the graduation requirement of University experimental courses, 90% of undergraduates will have to cry.

Then, in the presence of detergent, ensuring the coupling of composite crystal is another important requirement.

In history, kobika found that the solution of DMG could solve the coupling problem. How long it took him to solve this problem has not been recorded. However, with a lot of malicious speculation, it's a small idea to try for three or four years. Of course, it's not kobika himself. He has many doctoral students and assistant researchers, but this only shows how many excellent researchers' scientific research lives have been wasted.

After solving the coupling problem, the remaining steps, including enzymatic hydrolysis and so on, are not particularly time-consuming, but in terms of the normal historical process, a few months is always necessary. Yang Rui's operation of golden finger is also based on two months to reserve time.

However, all these, all these golden fingers, the light of genius, the painstaking efforts of scholars, under the dog's luck, are as ethereal as smoke and dust.

Gu Qiang went step by step. The correct steps were more than the trial and error steps. He did it, he did it, he did it

In Yang Rui's opinion, it's like you installed a very difficult stand-alone game, and found the clearance strategy from the Internet, and then... Three lives clearance!

"I'll take another look." Yang Rui called the roll and said: "Su xiankai, fan Zhenlong, Tian Bing, that... Professor man Hai, Professor Tao Xuelin, you also add in, and do a repeated experiment according to Gu Qiang's experimental steps. Lao Xu, you and I will check the experimental records again. By the way, the experimental table is not allowed to move, the garbage is not allowed to pour out, and the packaging of all used consumables should be recorded. "

The researchers who were named gathered together.

Gu Qiang still didn't realize his strong luck today. He laughed and said, "I don't need so many people. It's very easy for me to do this experiment."

"Well, Gu Qiang, open another group yourself and do a set of repeated experiments." Yang Rui didn't dare to take it lightly. This is another achievement of Nobel Prize. It was made in a few days. Who dares to believe it?

Gu Qiang casually changed the experimental table, took all kinds of consumables again, and began to do it. He said, "if you tell me something else, I'm not afraid to do the experiment."

While talking, Gu Qiang hummed a song.

Only Yang Rui, as if facing the enemy, stares at each experimental table in the laboratory, his mind turns.

The schedule on the white wall is as new as ever, only at the bottom. Two participating laboratories are added, namely, the University of Melbourne in Australia and the winter Institute in the United States. The progress bars are 2.7 points and 1.6 points respectively.

Yang Rui takes a look at it and doesn't look at it any more. If triple repeated tests can prove the accuracy of the results, the progress bar of the ion channel Laboratory of Peking University will be approved as 100 points.

……