Chapter 1221

Sleepwalking Dafa is often heard in science stories, most of which are touching scenes and feelings in sleep.

For example, the discovery of benzene ring, the invention of PCR, and even the discovery of DNA double helix are all successful cases of sleepwalking.

Researchers dream back in the middle of the night and wake up after a long sleep to solve the problem they have studied for a long time. This kind of Epiphany like model is very popular in all kinds of scientific stories, and ordinary people also pay for it. His implied message is that ordinary people can actually make outstanding scientific research achievements.

As a result, young people, middle-aged people and old people who are interested in science will laugh at scientists' sleepwalking stories.

Of course, ordinary people can't make outstanding scientific research achievements.

Ordinary people can make some small inventions, such as adding an eraser to a pencil. There is no problem, but the coverage of basic science has exceeded that of ordinary people. There are a lot of people who can't get in touch with the world's cutting-edge science even though they have a doctor's degree. Let alone the actual discovery process, they still have to follow the scientific process.

However, the second of sleepwalking lies in that it is a reasonable and legal backward push. I think the double helix is very handsome, so I'll try it. It turns out that the double helix is really OK, which is the best guide for sleepwalking.

Yang Rui hopes to make a three-dimensional structure of GPCR. The first step is to make a conformation of the transmembrane region of GPCR.

To put it bluntly, such a conformation is worth half a Nobel Prize.

If there are enough basic biologists to make a conformation of the transmembrane region of GPCR, it will almost win the Nobel Prize. Of course, it is right to share it with others.

It is such an important scientific achievement, but the conformation of the transmembrane region of GPCR is a very simple image when it is displayed in public.

Just like the DNA double helix, which is formed by the winding of two snakes, the conformation of the transmembrane region of GPCR can be described as the image stirred up by seven springs.

"The molecule that can transmembrane seven times" is a powerful protein molecule comparable to seven times a night. It may be the most powerful molecule in human body, but it is finally composed of seven tentacles, each tentacle is like a thick spring wire, and the image is not really good-looking.

However, such an image is not what you want.

The tentacle monster made up of seven tentacles is easy to draw, but the structure of GPCR should stand up to scrutiny.

What Yang Rui is going to use is sleepwalking. He is not really going to sleepwalk. It is in line with the current cognition and judgment of the scientific community.

This is what Yang Rui is looking for.

He had to see what he had to rely on for "sleepwalking.".

Three intracellular rings and three extracellular rings were understood in the 1950s and 1960s. The first two were smaller, and the third was larger. It took several years to determine. As for the transmembrane helix lamellae of receptor, helix I-IV formed one lamellae, helix V-VII formed another lamellae. This understanding is relatively new.

In addition, the most important is the image data from the electron microscope and X-ray.

Although protein is such a small unit, the technology of the 1980s was enough to make a rough picture.

However, the transmembrane conformation of GPCR focuses on transmembrane.

What scholars want to know most is why GPCR can span seven kinds of membranes.

This is also the core problem of transmembrane conformation.

While looking at the materials, Yang Rui is sketching on the paper, which is quite the opposite of what other scholars have done.

In order to study the transmembrane conformation of GPCR from scratch, scholars of this era should first imagine the placement of seven antennae in their minds, then consider the relationship between them, and then examine whether they are consistent with the known structural information, And explain the discrepancy

This is a very tedious and detailed and even irritating work.

DNA There are only two chains in the double helix structure. There are seven chains in the G protein coupled receptor, which is played by many laboratories. We can imagine the difficulties.

However, what Yang Rui wants to do is not addition, but subtraction.

After 30 years, the transmembrane conformation and the three-dimensional structure of GPCR are known, not to mention the conformation of the first step.

But Yang Rui can't take out the conformation of later generations directly, because some restrictions are unknown to the current academic circles.At the same time, Yang Rui also has to determine the research progress of his laboratory, which has reached a corresponding level. It's better to infer the conformational data from the research progress of his laboratory. In other words, he wants to draw pictures, but in the final analysis, all the images should be expressed by numbers.

And numbers, numbers naturally have to be based.

Yang Rui turned over all the information, and only then managed to get 70% or 80% of the content. As for the rest... Naturally, he had to force others to do it.

"When I went to bed last night, I thought about it carefully. The connection of the seven helix chains must have a certain role. I tend to think that they are used to recognize the substrate, and they are specific to recognize the substrate molecules..." Yang Rui said while writing two lines on the blackboard of the laboratory

1 、 The contact of 7 helices with the substrate was determined.

2 The local conformation was described according to the substrate molecule.

Simple 20 or 30 words, like a container hit on the jeep, pressure people breathless.

"That's all you think about when you sleep?" Gu Qiang looks at two questions and wants to make complaints about it.

Yang Rui nodded steadily, sleepwalking is good, the good thing is that the idea is simple to explain, without a series of logical problems.

Gu Qiang was very helpless and said, "I wonder how you can sleep."

Yang Rui coughed twice and said, "if you give the answer, I'm sure I can sleep."

"There's no ready-made way, is there?" Professor man Hai asked.

"No

"So it all depends on trial?"

Yang Rui shrugged.

In order to test the contact between the seven helices and the substrate molecules, it is necessary to test the substrate molecules and G protein coupled receptors. There are hundreds of substrate molecules, and the test is even more boundless.

Professor man Hai just felt dark in front of his eyes and murmured, "no wonder you want them to make so much bovine rhodopsin."

"It's still our advantage period now. We'll add more fuel. When the project is over, everyone can go abroad." Although academician Zhu came forward to do his work and his overseas trip was rejected, it was still very demoralizing. Yang Rui could only take the opportunity to draw a cake.

Man Hai also took the opportunity to show his loyalty and said, "as long as you can do it, it doesn't matter whether you go abroad or not."

"To make achievements is the focus now." Yang Rui quickly pulled over the topic and said: "now I have some ideas about the conformation of GPCR. I hope you can do your work well and contribute to the completion of the project..."

Yang Rui didn't take out the whole composition of his sleepwalking flow, but he did some simple partial pictures, Pass it to several researchers.

A group of people silent research up, some do not understand, at a loss of staring at the eyes, but also can understand, at a loss to start looking for old information to do comparison.

Yang Rui doesn't care. Instead, he feels slightly drunk after drinking.