Chapter 1212

"Crystallization is the most important step. We must pay attention to it again, remember the process and recite the steps in our mind..."

"Dissolution is the most important step. We must pay more attention to it and see if there is precipitation? Don't waste raw materials. In addition, always remember that solvent is not allowed to be added after one dissolution, so the usage must be calculated

"You don't want to calculate for me here, experiment, you have to do it!"

Tao Xuelin orders in circles in the ion channel laboratory.

This is what he is very good at. Before he became a full professor, Tao Xuelin's main job was to lead the people under the professor. Of course, before the 1980s, the most important work was actually the cooperation of various units and the leadership of the higher authorities. However, any leader who has any idea, whether it is the leadership of Peking University, the leadership of the Ministry of education, the leadership of the Ministry of culture, the leadership of the Academy of Sciences, or even the leadership of Beijing, can form a team to tackle key problems.

Ten years ago, the projects of the research team were various, especially for the teachers in the Department of biology. Simple, how to improve the effectiveness of manure, complex, how to design a brand new manure, more difficult, what can produce better manure

At that time, I worked on projects, It's not a school or a research institute that works independently. Although many projects can be done by only one or two people, in the eyes of leaders, how can the projects done by one or two people show their importance, and how can the projects that are hard to come up with be replaced.

The signboard of Peking University can not be ignored. Therefore, for a long time, Tao Xuelin has been doing "big projects".

There was a time when Tao Xuelin was depressed. In fact, there was a time when many academic elites were depressed. They always felt that they had wasted their time and talent

However, experience will never be wasted.

Although Tao Xuelin has done a lot of useless big projects, his experience of big projects has been preserved after all.

In the ion channel laboratory, he soon found his own position, which is to manage the production of bovine rhodopsin.

How to produce is Su xiankai's work. In his early days, he tried various ways while producing.

With the progress of the experiment, the nature of Su xiankai's work has changed slowly.

Just like Yang Rui's production mode of coenzyme Q10, Su xiankai's research is also gradually biased towards the research of production mode. Moreover, when his research scope is more and more extensive, bovine rhodopsin actually becomes the same as coenzyme Q10, and can work in a division of labor and enter a professional production state.

In fact, biopharmaceuticals were all produced in the laboratory in the 1980s, including Xijie factory established by Yang Rui. With a total of 10 to 20 people, 20 to 30 people, the production department of the factory can be established. The instruments operated by everyone are similar to the enlarged version of the laboratory. Some of them even use the laboratory for production without amplification.

Coenzyme Q10 extracted from bovine heart, insulin extracted from bovine pancreas and bovine rhodopsin extracted from bovine eye are all the same models.

With a little help, Yang Rui and Su xiankai gave Tao Xuelin all their current skills.

Tao Xuelin only took a few days to increase the production capacity of bovine rhodopsin several times.

This is not an initial increase of several times, but a further increase after su xiankai had been deeply cultivated for a long time.

With the addition of Tao Xuelin, the yield of bovine rhodopsin was not only 100 times higher than Yang Rui's requirement, but also more than that.

This is the embodiment of Tao Xuelin's management ability.

The same is the allocation of six research dogs, the improvement of Guqiang group is not so obvious.

Gu Qiang's experimental ability is strong, but his management is not strong. On the contrary, because Gu Qiang's personality is too strong, these graduate students brought by Tao Xuelin do not like the cooperation with Gu Qiang very much.

However, no one dares to listen to Tao Xuelin's orders. Unless someone doesn't want to graduate, for graduate students, a tutor is heaven, a tutor is earth, a tutor is a brilliant and great flower, and a tutor is a person who has been tortured to death thousands of times in your dream, but makes you pretend to enjoy yourself in the daytime.

Tao Xuelin is also in a good mental state. Putting his laboratory aside and entering Yang Rui's laboratory is also a great challenge for Tao Xuelin, who has seen him in his 40s. It's a pleasure to be able to find your position quickly.

He paced in the big laboratory he was assigned to, looking at the students' work one by one, more serious than when he was in his own laboratory.Gu Qiang was in a bit of a mess. He was angry and helpless. He looked at the energetic Tao Xuelin and said, "it used to be su xiankai, but now we have another Tao Xuelin. Now the number of bovine rhodopsin proteome is more than us. How can we use up so many materials?"

Tian Bing glanced at him and said, "there are too many materials. I haven't heard of them."

"Just ask if you are afraid." Gu Qiang asked while working.

Tian Bing hummed twice and said, "there's nothing to be afraid of. Director Yang has no requirement for me."

Inserting soluble protein fragments into G-protein coupled receptors is simple to say, but not easy to do.

G Protein coupled receptor is still a kind of protein, and the size of protein is far smaller than that of microorganism. In other words, you can't see it with ordinary microscope.

It's not easy to insert something into what you can see, but it's too difficult to insert something into what you can't see.

Therefore, it is even more difficult to verify the stability of the receptor.

Tian Bing himself is painful, but this does not prevent him from ridiculing Gu Qiang.

Gu Qiang curled his lips and said, "Yang Rui doesn't have any quantity requirements for you. It depends on the fact that you haven't made any achievements."

Tian Bing smile: "we just start to do it here, what do you do? If you can't make any more achievements, let alone Yang Rui, Su xiankai will have to kill you. "

Stay up late to do the bovine rhodopsin protein if wasted, Su xiankai really will be angry hit.

"I couldn't do it. I stirred up the bovine rhodopsin he sent me." Gu Qiang is very cruel. He is really tired recently, especially after Tao Xuelin joined in. Let alone sleeping, he always trots to eat.

Tian Bing laughed and said, "who can't waste? Be careful director Yang will hang you up and fight you."

Hanging up to fight is a word Yang Rui used to say recently, and others have learned it.

Gu Qiang argued: "who said a pot of stirring is a waste? Anyway, it's going to be centrifuged in the end. The preliminary treatment is carried out in a unified way, which means that the yield is lower and the efficiency is improved, right? Besides, after the increase in the number of students, the overall effect will not be too bad if they are treated in a unified way in the early stage. You don't see how many students are white when they are pretreated... "

"If I didn't know you were talking nonsense, I would have believed it."

"I can't say it's all bullshit..." Gu Qiang said himself and fell into a state of meditation.

"What do you think?" Tian Bing had a bad feeling in his heart.

Gu Qiang didn't say a word. After a while, he said, "in fact, if you think about it carefully, I'm not talking nonsense just now. What I said is some truth."

"What's the point? You're wasting at least 10% of your protein like this. "

"Not necessarily. Preprocessing is originally lossy. After batch preprocessing, the loss is a little larger, but we can find a way to optimize it. Compared with before, there will be 5% more at most.... "

Gu Qiang thinks about the future with a beautiful smile on his face.

Tian Bing reminded: "5% is not bad. Su xiankai, their output has increased to more than 100 times of the original. If you think about it, if you increase the loss by 5%, you will waste the previous production by five or six times. "

"When bovine rhodopsin is not enough, 5% must not be wasted. Now the conditions have changed, right? What's Yang Rui's purpose of increasing our output? Su xiankai and Tao Xuelin are busy making bovine rhodopsin for what? Not yet... "

"It's not for you to waste."

"To give us choice." Gu Qiang Zhen has a word. The more he says it, the more reasonable he feels. After a while, he nods his head and says, "I think we can have a try."

Tian Bing's eyes jumped desperately and asked, "what do you want to try?"

"As you put it, it's all in one pot."

"You put forward a pot of stir, not me." Tian Bing quickly declared, and tried to dispel Gu Qiang's worries, saying: "you don't have to fool around. Now there are too many people. Like Tao Xuelin, you have adjusted the personnel arrangement reasonably. Even if you work overtime, you can't add a few hours. One pot stirring doesn't work. It's not only the loss of pretreatment, but also the pollution in your later stage. What can you do when it comes to separation and treatment? "

"Do as you should." Gu Qiang said, pausing for a moment, and said: "before we did it separately, it was like cooking in the kitchen. You need to prepare each dish one by one. Each dish is prepared in a different way. It's like the hot pot, the copper pot of DongLaiShun. Didn't you eat a lot last time? Is hotpot worse than stir fry? "

Tian Bing looked at Gu Qiang like a madman and said, "you are driven crazy by the task.""Yes, I was driven crazy." Gu Qiang lay down, clapped his hands and said, "in a word, I have to have a try."

"If Yang Rui knew that you had wasted so much bovine rhodopsin, he would hang you up and beat you."

"Hot pot, is cooking bad?" Gu Qiang asked again.

Tian Bing said, "you don't want to explain it to Yang Rui like this."

"When it comes to hot pot and stir fry, Yang Rui should understand it better." Gu Qiang consciously found a perfect reason.

……