Chapter 1203

"Old sue, we don't have enough protein here." Fan Zhenlong came around from one side of the experimental table, gave a cry, and then went back to work.

Su xiankai hummed twice and complained, "how can I use it up again?"

"I can't help it. I can't get results all the time." Fan Zhenlong bored way: "our side of the protein dosage to exceed the plan."

Su xiankai's eyebrows twitched twice and said, "I'm not sure I can do the planned dosage. If you exceed the plan, what should I do?"

Fan Zhenlong said helplessly: "of course, we don't want to go beyond the plan... We can only say that what we thought in the early stage was too simple. The three-dimensional structure of this thing is much more difficult than expected."

"It's really difficult." Gu Qiang was very reluctant to admit it, but he said: "our original plan may have to be completely revised..."

"Don't change it." Yang Rui himself is constantly scanning the literature, said: "the new order of equipment will arrive soon, in addition, Lao Su, give you two more people."

At the beginning of his plan, Yang Rui knew that he was going to overspend by a large margin, and he was well prepared.

He spent half of his $6 million on budget, and the rest was for the reserve team. Moreover, he still had a large amount of RMB to spend. It was no problem that the funds for the ion channel laboratory, together with the funds appropriated by the 863 plan, would make up three million yuan.

If it's not enough, he estimates that he can get another sum of money from Peking University. In theory, he can also get a lot of money from the domestic central enterprises of biomedicine and animal husbandry and even ministries and commissions.

This is the advantage of reputation. As long as the project is successful, all the donations are for nothing.

As for the failure of the project, the loss is reputation itself.

In fact, Yang Rui doesn't know how much money is needed to achieve the desired results. However, he knows how much foreigners spend. Therefore, he has a considerable estimate of the difficulty of each link.

As soon as Su xiankai turned his head, he knew that Yang Rui had been prepared, which made him slightly embarrassed.

Yang Rui saw it. He was embarrassed by Su xiankai for a moment and said, "our plan is based on the first-class laboratories abroad. Now, we haven't reached the level of the first-class laboratories abroad. It doesn't matter. The more funds I prepare, the more resources I prepare, is to make up for the gap between us."

After a pause, Yang Rui added: "GPCR is a world-class project, and it will eventually compete with foreign first-class laboratories. Therefore, everyone, including me, should grow up as soon as possible."

When he said that, Su xiankai's expression eased down.

However, the pressure of reality quickly came on Su xiankai.

"Just adding two people and a batch of machines can't make up for the gap between us and foreign first-class laboratories." Su xiankai is aware of the loopholes in Yang Rui's logic.

Yang Rui curled his lips and said, "if we do the project like a foreign first-class laboratory, it will not work. However, our goal is to concentrate the materials on bovine rhodopsin."

Several people in the laboratory are not clear, so they look at Yang Rui.

Su xiankai asked: "our material is not bovine rhodopsin?"

"We are, but not necessarily in foreign laboratories."

"Why?"

"Because they want better materials and want to try more templates, this is our chance." Yang Rui still has a word to say, that is, he knows that at this stage, bovine rhodopsin is the best material.

G Protein coupled receptor, as a protein that can transmembrane seven times, has a very complex molecular structure, and this complex molecular structure is also the reason why it hinders human research.

Bovine rhodopsin is the most stable and clear seven transmembrane protein found by scientists.

At least at this stage, it is.

Of course, scientists have unlimited wildness. They always want better materials.

Yang Rui also wants to explore such a world when his laboratory becomes the world's top laboratory, even at twice and triple the cost.

In China in 1986, the ion channel Laboratory of Peking University was not qualified to do so.

Ordinary laboratory, select a material, or in order to spread the risk, select a variety of experimental materials, directly carry out the experiment, is the common practice.

Yang Rui gives his confidence to bovine rhodopsin protein, and that's all.

This kind of knowledge, for Yang Rui, is natural, but for Su xiankai and others, it is a little rare. After 30 years, domestic graduate students can learn a lot of relevant information from their classmates and teachers even if they don't read relevant papers. However, in China in 1986, there was no such condition.How do foreign first-class laboratories do experiments and how do foreign top-level laboratories do scientific research? Domestic ordinary scholars have no concept at all.

Not to mention the laboratory management, all belong to the confidentiality.

However, Yang Rui is very clear that in the field of GPCR, everything is chaotic, as it was in 1986 and 2016.

However, Yang Rui can see a little more information than others.

For example, in the chaotic field of vision, find the most appropriate material.

"I asked people to send more ox eyes." Yang Rui stood up, made a phone call, politely hung up and said, "it's done, Lao su. Let's double the scale. Is that ok?"

"Double?" Su xiankai some can not adapt to the way: "do we want to step by step, first increase about 20%

Yang Rui sighed and said, "if you double it, it will come step by step."

He took a look at Gu Qiang and fan Zhenlong, and said, "don't listen to them say that it's true that it's twice as much as expected. Judging from your current progress, I think it's far from enough for you to provide twice as much bovine rhodopsin."

Gu Qiang was not happy and said, "director Yang, you look down on people."

Yang Rui picked his eyebrows and asked, "say something that I can look up to. What step have you taken now?"

Gu Qiang snorted for a long time and said, "we are close."

"If it wasn't for your experience, do you think I would give you more materials?" Yang Rui half is about to give a word of encouragement, said: "don't be wordy, you also have to learn a lesson, don't let Lao Su's hard work in vain."

Su xiankai's face had already collapsed, and he said: "I feel like a coolie. Director, how can I say I'm also the Deputy group leader? Can't we give me a more energetic job?"

"Your work is energetic enough. In addition, our main work recently is to produce bovine rhodopsin, so your main direction is..."

"You lied to me." Su xiankai was full of suspicion.

Yang Rui solemnly said: "in the next month, or two months, the place where funds, materials, instruments and personnel will be increased is to produce bovine rhodopsin. Do you understand?"

Su xiankai now believed it. He just frowned and asked, "why?"

"Why? Because your teammates' experimental level is low, they do it slowly and consume a lot of money. If they don't have other good ideas, they can only feed them with materials. "

One after another, Yang Rui finally inspired Gu Qiang.

Comrade Gu Qiang clapped his case and said, "you have a good look. We don't need to feed. The next day, I will raise the success rate."

"Not bad. I can speak Beijing dialect. Erhua's pronunciation is decent." Yang Rui suddenly put a smile on his face, which caught people off guard.

Gu Qiang can't help choking. He can't talk about Er Hua Yin.

Xu Zhengping coughed and asked, "Yang Rui, do you really want to double the production of bovine rhodopsin?"

"Yes, this is our only chance to catch up with foreign laboratories." Yang Rui deliberately said something more serious, and then said: "we should realize the strength of the enemy, and we should not underestimate ourselves. Your success rate of experiment is indeed not high, but what about the researchers in foreign laboratories? How many times can they make it? It's also something that doesn't work. It's a big project. There are still many experiments to be done. It's a long time. We need to gain an advantage from the source so as to eliminate the enemy's other forces, right? "

"Foreign laboratories, do not want to improve the success rate from the source?" Fan Zhenlong asked.

"First, foreign laboratories are not sure that the most appropriate material is bovine rhodopsin. Second, even if they realize it, they are also pinning their hopes on the success rate of the next experiment. Third, they can't catch up with us in obtaining fresh ox eyes. "

Finally, Yang Rui said it half true and half false.

To some extent, after Yang Rui established his genetic engineering laboratory, his relationship with various animal husbandry companies has been able to provide him with a large number of animal materials, limited to such a small point, which is the top condition in the world.

Fan Zhenlong was stunned when he heard Yang Rui say the second point. He knew Yang Rui's speaking habits too well.

Fan Zhenlong asked suspiciously, "what do you mean they are pinning their hopes on the success rate of the next experiment?"

"Literally." Yang Rui coughed.

"No, you said hope. You mean there should be no hope."Yang Rui chuckled twice and didn't answer directly.

Fan Zhenlong asked again and again.

Yang Rui considered the language and said, "well, it's quite accidental."

It's not just accidental. The three-dimensional structure of GPCR, like all kinds of star exploration projects, is piled up in quantity.

In what quantities?

Bovine rhodopsin!

In fact, the top laboratories abroad have lagged behind in this respect.

Only when they realize that bovine rhodopsin is the best material, and only when they realize that they need a lot of materials to pile up the three-dimensional structure of G protein coupled receptor, can they catch up with the progress of ion channel laboratory.

Of course, once foreign top laboratories realize this, they can catch up very quickly.

Yang Rui can only run as fast as possible.

Fan Zhenlong was completely shocked by Yang Rui's answer.

He hesitated for a long time and asked, "how accidental is it?"

"Don't worry. I'll organize the production of enough materials." Yang Rui patted fan Zhenlong on the shoulder to ease his mind.

Fan Zhenlong's heart is going to explode!