Gu Qiang according to the plan to operate, first of all, do synchronous estrus.

Estrus synchronization is to use some exogenous hormones to artificially control the estrus cycle of a group of female animals, so as to organize embryo transfer in a planned time.

There are many drugs that can be used for exogenous hormones, but the control effect is strong or weak, and the efficiency is high or low, which is not very rare. What Yang Rui discussed with Professor Wang Yong on the same day is that the existing exogenous hormone is easier to use the ready-made natural hormone than the newly developed one, not to mention this kind of mature medicine which is not a key part.

Of the 200 Simmental cows at the scene, 186 have entered the estrus stage, with a success rate of more than 90%. It is not easy to improve it.

The remaining 14, at a loss, looking at their companions, don't know why they suddenly changed.

A young cow is anxious to turn around.

She is surrounded by estrous cows, constantly moo, bow, the spirit of impatience, a look is not the same as usual.

This young cow, after the 20th or 25th turn, resolutely made a decision. She also arched her waist and learned from other cows to give up the feed in the trough. Then she kept raising her hooves and turned her head to the cowshed where the bull was.

Finally, the heifer successfully attracted the attention of a veterinarian.

He put on heavy rain shoes again, took the equipment and went up to check.

Gu Qiang also raised his head and asked, "are you in heat? That's 187? "

Comrade Qin Yue, a veterinarian from Zhongmu, looked at the cattle for a while. He came back and took off his glasses and wiped them. He said, "no, 186 heads."

"No? It looks like it. "

"It does." Qin Yue put on his glasses and gave a concise answer.

The crowd around the bullpen was laughing softly.

Gu Qiang shook his head in amazement.

He spent a long time in the laboratory and a short time on the spot. However, most of the researchers are like this. They are often sent to the site by the lowest seniority members of the research group or project group, or assistant researchers, or doctoral students, or graduate students, sometimes even undergraduates.

However, genetic engineering laboratories do not have junior lab dogs to exploit.

In the genetic engineering laboratory, Yang Rui has the lowest seniority. He only has three years of scientific research experience, which is similar to that of ordinary master's graduates. In the Research Institute, he is equivalent to the qualification of assistant researcher. Therefore, Gu Qiang can only do it in person.

Fortunately, for the sake of 200 Simmental cattle, China animal husbandry Corporation will complete the auxiliary manpower allocation.

When Gu Qiang called "superovulation begins", there was a herdsman who brought the cows one by one after oestrus, weighed them first, and then recorded them.

Four veterinarians from Zhongmu, in a row, injected the cows in turn.

Qin Yue was at the top of the list. He was not only responsible for injection, but also responsible for reporting numbers and judging the health status of cattle.

Gu Qiang is standing behind the veterinarians. According to the weight data reported, he sucks gonadotropin into the syringe and hands it to the veterinarians.

Yu Xing'an is the one who helps Gu Qiang. He stares at the veterinarians and ensures that all the medicine in the needle is injected into the right cow.

By the time the whole injection process was completed, Yu Xingan poured a large amount of foam water into the veterinarians' body in the name of disinfection.

Even if someone stealthily squeezes the medicine on his body, the whole barrel of disinfectant will destroy the activity of the medicine.

Director tie, who is from Qingmu, has been reporting on his work in the capital for some time recently. Today, he came here specially. Seeing this scene, he could not help but smile and said to director Mei, who is proud and rugged beside him: "look, no matter how much money you Zhongmu people give, you are still not trusted."

"You don't have to sow dissension. It's too tender for you to play." The proud and rugged director Mei has always despised the resourceful ability of the broad shouldered and strong iron director.

Iron director ha ha a smile, way: "need not stir up dissension, you see, a gonadotropin, they all hide, afraid you know."

Director Mei's face was also a little ugly. He said, "they may have developed some special hormones. If they don't let other units know, it's normal."

"What if you can get five at a time? I heard that there are six gonadotropins and seven gonadotropins for ovulation, which are the latest technology in foreign countries. They don't use much. It's a big deal to buy them. How much money do you give Director tie doesn't care about the relationship between Haidian District genetic engineering laboratory and China animal husbandry Corporation. Now he just wants to meet Comrade Mei. Of course, if the relationship between the two sides is bad, it would be better. He dreams of monopolizing this resource.Director Mei has not dealt with director tie since he went to school, but he secretly agrees with what he said just now.

Director Mei twisted his neck uncomfortably and said: "Yang Rui is a young man after all. This Haidian District genetic engineering laboratory is also a new laboratory. They have developed new technology with great difficulty. Even if it is only 60% of the efficiency of foreign products, it is worthy of respect."

"Hey, the people who work in Beijing are different. There are a lot of official words." Director tie laughs twice, but shouts to the veterinarian in front of him: "see the egg? How many eggs

He asked, of course, how many ovulated.

Those who have studied biology in junior high school all know that the embryo comes from the combination of a sperm and an egg. The number of sperm released by a bull at one time is extremely large, it can be said that it is tens of thousands, and the eggs are extremely scarce.

For a cow with only one embryo per birth, the number of eggs in a cow is less, often only three to four. In other words, the number of embryos available for embryo transfer is actually determined by the number of eggs.

If a cow ovulates 4 embryos at a time and all of them develop into A-level embryos, it is possible to get 8 half embryos after one embryo division.

Even if the success rate of half embryo transfer is 50%, for improved breed cattle, it is also double the breeding efficiency, let alone multiple ovulation within a year.

However, commercial operation has always been unable to achieve the ideal data, and the desire for capital can not be satisfied by four or eight times efficiency.

In developed countries other than China, the reason why biotech companies are tirelessly spending money on embryo transfer technology is that they have improved the success rate in all aspects.

For example, in the process of collecting embryos, the purpose of injecting gonadotropin is to get more than four eggs

In an era when an egg basically represents a cow, it is inevitable to develop better and better gonadotropins.

From the 1970s to the 1980s, gonadotropins changed their polarity, not only to get more eggs, but also to reduce side effects and increase the proportion of usable embryos.

In this field, the domestic research institutes naturally fall behind again. It should be said that there is basically no research, and they have introduced and used the technology that came from Eastern Europe in the 1950s and 1960s.

However, the difference between five eggs and seven eggs is not one man, but two cows.

Veterinarian Qin Yue, stepping on rain shoes and carrying a notebook, came over and called "director Mei", then called "director tie", and then asked softly, "do you call me?"

"I called you. Director Mei wants to know how many eggs a cow has?" There was no expression on director tie's face. He was very serious.

"It's not clear yet." Qin Yue looked at his notebook and hesitated.

"Just say you know." Director tie has been a ranch all his life, and he has no leeway to speak.

Qin Yue turns his face to director Mei.

"Well," director Mei said, "if you ask, just say as much as you know."

"Yes." Qin Yue looked down at the book again and said, "we have checked 12 cows at present. The corresponding ovulation numbers are 10, 12, 11, 10, 13, 9, 12..."

"Wait, I asked you the number of eggs, how many are there in a cow?" Director tie stares.

Qin Yue asked for the frame and said, "I'm talking about a cow."