Chapter 1076

It's very difficult to do primer work. Maybe it's not as difficult as "seventy-seven forty-nine boys and girls do medicine introduction", but it's also more difficult than rootless water from the desert.

In the era after the rapid development of computer technology, biologists have developed special software to make primers, such as premier primer 5.0, oligo 6.0, vector tisuit, dnasismoga, DNAStar and so on. Just look at the number of software, you can imagine how complicated this is, These softwares have not solved all the problems.

In fact, even though PCR has been invented for 30 years, the design of primers is still not a routine science. Of course, scholars have summed up some rules, but uncertainty and contingency still perplex scholars.

In the early days of the establishment of genetic engineering laboratory, Yang Rui didn't require working hours. Now he is offering a magic weapon of 110 hours again because of this uncertainty and contingency.

Uncertainty and contingency can be summed up as probability, and the most natural way to solve the problem of probability is to increase samples.

The researchers of the whole genetic engineering laboratory devoted themselves to the work of primer design.

It is Yang Rui's reward to publish papers in first-class Chinese journals. Correspondingly, hard work is inevitable.

On this point, Yang Rui is very magnanimous.

There is a huge gap between Chinese laboratories and those in developed countries. From funds to instruments, from management to technology, from environment to accumulation, the only reason why Chinese laboratories can still make world-class achievements is the blood and tears of experimental dogs and researchers.

Modern laboratories all have access control systems. Whether it's clock in, fingerprint brushing, or cow iris, they can record the working hours of experimental dogs and researchers. If we compare the attendance time of Chinese laboratories and foreign laboratories, we can clearly see the gap.

Most of the foreign laboratories work eight hours. The more developed the laboratories are, the more fixed the working hours are. If we investigate the domestic laboratories, Tsinghua biological laboratory, the doctoral students' attendance time is about 450 hours per month.

The 450 hour attendance per month is much harder than the 110 hour attendance per week, which means that the doctoral students in Tsinghua university can't rest more than three days a month, and can't sleep more than eight hours a day. Besides eating and sleeping, the experimental dogs must give all the time they have to the laboratory.

If science needs sacrifice, its sacrifice is the time, blood, tears and hardship of experimental dogs and researchers.

After finishing his master's degree, Yang Rui didn't continue his further study. A large part of the reason is that he can bear the time and the blood and tears, but it's hard for him to bear the hardships in his study.

Doctoral students with an average age of more than 25 years old are already standard adults. However, they can only earn about 1200 yuan for 450 hours of labor per month. It is not too much to say that they are in hardship.

Yang Rui, who used to struggle, still can't get out of the quagmire of real life. When you go back to the dormitory after a hard day, tired and thirsty, and pick up a box of luncheon meat, but can't bear to open it, the aura of academic success and academic dedication has faded.

It is also because of this experience that the first thing Yang Rui did when he returned to the 1980s was to solve his own economic difficulties.

When the economic difficulties are solved, Yang Rui has no scruples——

Even if he is faced with daunting primer design, it is no exception.

To be honest, the help Yang Rui can get from the database in his mind is very limited. At most, he can get some theoretical guidance.

Of course, theoretical support is also valuable. Theoretical guidance not only pointed out the direction for Yang Rui, narrowed the scope of his attempt, but also gave him great confidence.

Even so, the actual operation is still difficult.

At least it is many times more difficult for scholars to do the unknown primer after 30 years.

Later scholars at least have primer design software available, only this one, do not know how much time to save. There are more templates for future scholars to refer to

In 1985, Yang Rui couldn't even pursue the efficiency of the instrument.

However, Yang Rui has no fear of difficulties.

He has enough to eat and wear, beef to eat, custom-made windbreaker to wear, want to ride a car, want to ride a bicycle, he also has two rooms of stamps, his own laboratory and valuable patented technology.Even if he devoted all his time and energy to primer design, even if it took months or even years, Yang Rui didn't feel afraid of difficulties.

What's more, he's not working alone.

After all, Yang Rui is in charge of the genetic engineering laboratory, and the eight researchers in the genetic engineering laboratory are also valuable helpers.

Later scholars who do primer design are not good. Many biological experts have experience in primer design. Indeed, they have more technical means and data to support them. But it's good for scholars who do such research to have an assistant. If they want eight professional researchers to pay 110 hours a week for help, they don't even need to think about it.

After all, the God of science heard Yang Rui's prayer. After sucking the blood, tears and time of eight researchers, he gave back to the genetic engineering laboratory, slightly making up for the lack of technology and equipment.

The only thing Yang Rui can guarantee is that his researchers will not suffer.

Although he can't give extra salary to the researchers in public laboratories, it's easy to get more benefits like the state-owned enterprises.

Enterprises like Zhongmu are also willing to meet Yang Rui's requirements.

While the researchers were around the experimental table, Yang Rui made a few phone calls, turned around and said with a smile, "I congratulated director Mei, director tie and director Qu, and invited them to see the demonstration after the agreement. In addition, director Mei promised to offer us another cow as a gift. Of course, it's good to kill it. "

The researchers in the lab laughed.

"Director Mei is a good man. He also delivers beef."

"We haven't finished the last beef at home."

"You don't know how to eat. I'll tell you that Zhongmu sends all the fat cattle. You can't just cook them. They taste good in the oven. It's the stove we use for heating in winter. Do you have an oven in your house

When it comes to eating, the atmosphere on the experimental table suddenly becomes harmonious.

Yang Rui is also smiling, and so on a few people in turn read the experimental records, he motioned to turn on the electrophoresis apparatus again.

Primers are invisible to the naked eye. A weaker microscope should not be expected. Moreover, it can not be seen or judged by ACTG. Therefore, the way to verify the success of primers is gel imaging, which is running electrophoresis in biological laboratories.

Electrophoresis can not only see whether the primers meet the requirements, but also the efficiency of the primers, which is the so-called specificity.

Yang Rui has just done it. At this time, he handed over the work to Gu Qiang and asked him to check it again.

Gu Qiang's experimental operation is not good. The accuracy is one order of magnitude higher than Yang Rui's. It's no exaggeration. We all believe in his operation. We watch Gu Qiang's actions one by one and get nervous again.

Yang Rui is also inexplicably nervous.

The false-positive data in the laboratory can't be more normal. Materials are polluted, or operations are wrong, and so on. The famous penicillin, in fact, is that the culture dish used in the experiment is polluted. If it falls in some laboratories, there must be experimental dogs hanging on the altar to bleed on the spot.

In this primer design, although Yang Rui used the data in his mind, there were no specific details.

Therefore, the primers Gu Qiang wants to test now are designed by Yang Rui independently.

Even in 20 years' time, this skill is better than that of ordinary Ph.D. head dogs. However, independent design means that Yang Rui can't compare the data again and again. At this time, he is a little uneasy.

If Gu Qiang did gel imaging, the specific bands of the electrophoresis map of the product were not obvious, or the band of the last two dimers would be lost.

However, if you lose face, you lose face. There are always times when you lose face in scientific research.

Yang Rui looked at Gu Qiang's rhythmic operation of the electrophoresis apparatus, but slowly relaxed.

In fact, when he first went to Professor Tang's laboratory, the most he did every day was the electrophoresis instrument. It was not easy to say how ingenious the operation was, but it was not easy to make mistakes.

Gu Qiang's operation is naturally better, just look at his action, it gives people a clean feeling.

When the electrophoretic map of PCR product comes out, the band is more clear

However, the focus of all people is only on that specific band.

"The output is very high..." several people praised.

PCR The product electrophoretogram showed a strip of "bar code" on a piece of black, and the "bar code" was white, long and short, arranged in turn.

The clearer the bar code, the more content of the substance.In current experiments, the clearer the specific band, the better, and the less dimer or hairpin structure, the better.

Yang Rui can't help but hold his chest up and look up. This is his design.

"Professor Ouyang, let's do a group of tests?" Yang Rui's self-confidence has greatly increased.

Ouyang Shi answered loudly.

Sun Zhengchu was envied and said: "if this experiment is done, I'm afraid it can be published in the top animal husbandry journals abroad. Journal of nutrition or British Journal of nutrition

Yang Rui said nothing with a smile. Although the Journal of nutrition is the top journal of animal husbandry, it is not the top choice for him.

"Let's see the results first." Yang Rui did not say much, but returned to the state of doing experiments.

……