Chapter 1067

"After you." Director Mei gave way, but felt that it was inappropriate. He added: "director yuan, Yang Rui didn't know you had done scientific research. This..."

"I understand, I understand." Director yuan laughed with the dignity of the superior and said, "I just can't say. We'll go back and I'll nag you again."

"You're joking. At that time, I'll put on the table and invite experts to listen to you explain the analysis to us." When director Mei saw that director yuan finally knew what he was doing when he asked him to come, he was a little relieved.

He wants to know the situation in the laboratory, but he doesn't want to offend Yang Rui.

In a moment, they registered with the guard and entered the laboratory.

Director yuan looked around, still disgusted, and said, "this lab is an old factory, isn't it?"

"It used to be a warehouse." Director Mei answered in a low voice.

Director yuan shook his head and said, "it's impossible to do this. For foreign laboratories, we have to do a good job in infrastructure construction, not to mention the advanced instruments inside. I think the paint on the top has fallen off, and other coatings have fallen down, affecting the experiment."

With a smile, director Mei said, "no way."

"Why not? Those who do experiments should pay attention to all these."

"I guess it's still no money."

"Didn't you donate money to them? Where's the money going? "

Director Mei was a little surprised and said, "do you know this?"

"Who doesn't know." With a smile, director Yuan said, "China animal husbandry Corporation has donated millions at a time. People in the circle are watching."

In this sentence, director Mei is a little nervous.

When he looked around, he could not help feeling that the infrastructure was too old. However, considering what Yang Rui had said before, director Mei explained on his behalf: "it is said that many steps of embryo transfer of this cow have to be done on site. The environment on the ranch is worse than here, so there's no need to spend a lot of money renovating the laboratory. "

"That's right." Director yuan pondered for a while, nodded slightly, but still despised his face and said: "in fact, in the final analysis, it's a word, poor. Our domestic conditions are poor and funds are scarce. These are objective conditions. Under the constraints of objective conditions, it is not easy to do research itself. Therefore, instead of learning from foreign laboratories to do embryo transfer, it is better to do some simple research, don't you think? "

The first half of director yuan's words was agreeable, but the second half made director Mei reluctant.

However, they had already entered the laboratory, and he could not quarrel with director yuan now. He could only nod his head and say: "here, Yang Rui is on the right side of the front."

"Quite young."

"Yes, I'm only 22 years old."

"Only 22 years old, there's a laboratory." Director yuan tut tut twice, said: "when I was 22 years old, I was still teaching below. I searched all over the county, but I couldn't get a bottle of concentrated sulfuric acid."

Director Mei nodded again with a smile, then said hello to the figure in front of him: "director Yang, I'm here again."

"Yo, director Mei." Yang Rui looked back and saw director yuan. He asked with a smile, "who is this?"

"Director yuan is the leader of our science and Technology Commission. He talked about it yesterday and came to see our laboratory today." Director Mei tried to make it light.

Yang Rui was a little surprised and said with a smile: "this is a leader's inspection..."

After a few nice words, Yang Rui didn't make the move of leadership inspection. In the final analysis, he has no subordinate relationship with the science and Technology Commission.

"Director Mei, director yuan will give it to you." Yang Rui has no deputy like Xu Zhengping in the genetic engineering laboratory, so he simply threw it back to director Mei.

"No, I'll just have a look around. Let's get to know your work." Director yuan came to see Yang Rui sit down in the experiment. He found a chair to sit down and observed.

Yang Rui is a little confused, but he still works as usual.

He has done the part of providing primers for PCR, which can not be learned by naked eyes.

In fact, most of the experiments can't be learned by eyes. Not to mention the complicated chemicals, people can't distinguish the type, content and proportion. Most of the time, primer design is based on luck.

The so-called primer is the primer in PCR reaction.

In order for PCR to work, primers must be used to lure out the required DNA fragments.

Therefore, primer design is the key step of successful PCR amplification. Good primers can not only avoid the production of background and specificity, but also recognize genome template and so on.However, the primer is artificial, just like the drug primer, it is always difficult to get.

In the 21st century, primer design is a relatively simple work. There are not only universal genes for reference, but also a large number of design templates, and even three huge international nucleic acid databases for querying the corresponding information resources.

Of course, there are corresponding primer design software for use.

Even so, the vast majority of scholars will not design their own primers, because the work is cumbersome and professional, they prefer to find a professional company to outsource the work.

Only when they are at the forefront of the industry and are not willing to disclose the breakthrough points and priorities they have explored, can they design their own primers. Of course, there are those who can't afford the funds, and the old school scholars will do the same.

In 1985, Yang Rui could not find any useful primers.

In fact, he can't find anything, let alone primer design software or corresponding nucleic acid database, which is the simplest general template. He can't even get one.

Now PCR research is in full swing competition - naturally, it is not competition for PCR itself, but competition for the scope of PCR.

It's just like when X-ray first appeared, a large number of scientists started from their own research fields and thought of ways to use it, so everyone rushed to do it, at least to make the simple part first.

There's no easier way to brush your face, brush your reputation and brush your influence factor.

In this state of fierce competition, you can't expect well intentioned scholars to publish their painstaking primer templates. Even if there is, it is usually incomplete.

Fortunately, Yang Rui himself had designed primers, and with the support of various literatures, he was qualified to do it.

Of course, during the period of PCR design, Yang Rui had to make some primer templates, but he couldn't use them in gender identification.

He's even going to have to start with the bovine genome.

This actually involves the genome project. The goal of the human genome project is to sequence every gene in the human genome and mark it like a map. Part of its function lies in the convenience of running PCR.

In 1985, there was neither a human genome project nor a bovine genome project.

Any unit or individual who wants to know which segment of the gene is sequenced.

In fact, this is not particularly difficult. The human genome is composed of 3 billion base pairs, so it costs a lot of time, manpower and money. The core sequence of a specific gene of SRY sequence that Yang Rui needs, even in the 1980s, only costs tens of thousands of yuan.

Of course, there are very few laboratories that can do gene sequencing. In China, Yang Rui's ion channel laboratory is also needed.

Ion channel laboratory has set up a special research group long ago to do a lot of gene related research. It is not very difficult for them to set up a separate project to do a special gene sequencing.

If other laboratories have requirements, they naturally need to negotiate and arrange time. Yang Rui has requirements. As long as the corresponding funds of genetic engineering laboratory are allocated to ion channel laboratory, the results can be easily obtained.

On this basis, Yang Rui began to design primers.

He spent most of his time doing paper work, and a small part of his time dealing with bottles and cans.

Of course, Secretary Mei was dizzy and director yuan was sweating.

Director yuan knows the PCR instrument and the principle of PCR. He also used the PCR instrument, but that's all.

Director yuan came back from England more than a year ago. At that time, PCR was made by Yang Rui. Therefore, director yuan did not come into contact with PCR and its use abroad.

Back home, director yuan has not been working in the front line for a long time. Although he maintains the tradition of reading journals and going to the front line frequently, he really doesn't know much about the emerging PCR.

Of course, if Yang Rui uses PCR normally, it will not be difficult for director yuan. It's nothing more than an X-ray machine. For scientific researchers, it's no more difficult to operate than an electric rice cooker.

However, when Yang Rui began to design primers, director yuan was blinded.

Most scholars who have a general understanding are blind.

And in the eyes of director Mei, director yuan was even more embarrassed.

"Let's go back." At noon, director yuan can't wait to walk out of the laboratory.Director Mei quickly followed up and asked in a low voice, "how are you looking? Are they on the way to fruition? "

Director yuan was silent and thinking.