Chapter 1064

"PCR is worthy of the Nobel Prize. It's so easy to use." Ouyang Shi stands in front of the PCR machine, just like a hungry person standing in front of the barbecue, with a gentle face.

Yang Rui stands in front of him with a smile and looks at Ouyang Shi's operation.

He was hurt when he played PCR in his last life. If he can't do it, he won't do it.

Gu Qiang did not have Ouyang Shi's deep feeling. He was operating the microsurgical knife across the experimental platform, laughing twice, and said: "you made this. It's not difficult at all. It's boring... Xiao Liu, take it away."

He divided another set of embryos and asked his assistant to send them away.

Xiao Liu is a researcher in the laboratory. However, he is not good enough, so he should be an assistant.

Of course, there are also advantages to being an assistant. He can learn some operation methods of microsurgical scalpel with Gu Qiang. If he learns 80% of Gu Qiang's skills in this technology, he can eat and drink all his life. Even in the 21st century, there are not many biotechnology service companies that can master this technology, and few are willing to use expensive bovine embryos to practice for you, We are all high paid skilled, a novice is not training mode.

Gu Qiang's embryos will be sent directly to another group for embryo freezing and thawing. Although it is still an experiment, it is also a practice step. Otherwise, they will not be frozen one by one.

Compared with Gu Qiang's embryo segmentation, the operation of frozen embryo is much less difficult, mainly due to the configuration and addition ratio of reagents. The latter is the main part of their training, while the former is Yang Rui's work.

In order to smoothly receive money from the technical service company in the future, Yang Rui deliberately concealed some key details, such as the configuration of the reagent, which was completed by him in the whole process. Other people had no formula and knew that other steps could not be repeated.

Compared with Gu Qiang, the workload of reagent preparation is much lower. Yang Rui can prepare a large amount of solution at one time, which is used for a long time with low cost and short time.

It took only a few days for the whole genetic engineering laboratory to enter the smooth mode, which is also the result of Yang Rui's experience.

Up to now, he has managed ion channel laboratory and Huarui laboratory respectively for two years, and his accumulated management experience is no less than that of other domestic managers.

Nowadays, some scholars in provincial laboratories are not necessarily as experienced as him.

Of course, Yang Rui's reputation is also very important.

For example, comrade Ouyang Shi was once known for his rebelliousness. When he was in Shanda, he was a little bit more than Guqiang. But in the genetic engineering laboratory, he was willing to accept Yang Rui's trend.

After hearing Gu Qiang say that PCR technology is not difficult, he was not angry, but also very happy: "good technology, mature technology, is to do simple, otherwise, how to promote the technology?"

"The technique of freehand segmentation is simple. Director Yang won't let me use it." Gu Qiang never forgets this. Compared with operating a microsurgical scalpel, the unarmed segmentation scheme is more elegant, but more comfortable.

Yang Rui had nothing to do under his hands. Looking at the two men's work, he said calmly: "there is no potential for unarmed segmentation. The scheme of microsurgical scalpel is well done. How fast the success rate rises."

"How can there be no potential to tap, I think I can make some progress." Gu Qiang said that he was a little proud. His experimental level, not to mention learning by himself, was no problem in a few laboratories.

Yang Rui laughed twice.

Ouyang Shi was not polite, immediately commented: "boast."

"Don't you believe it? Let's have a try? " Gu Qiang is about to put down his microsurgical knife.

"If the embryo is damaged, the funds will be deducted." Ouyang Shi laughs and holds Gu Qiang's seven inches.

Gu Qiang bowed his head helplessly and could only operate at the same time, saying: "I can do two experiments at the same time, and I can do it."

"What if you can do 10 experiments at the same time? If you can come up with a technology like PCR, it's the real one. " Ouyang Shi gave a thumbs up and said, "do you know why PCR is qualified to win the Nobel Prize?"

"Oh?"

"Because PCR promotes the development of biology and human beings..." Ouyang Shi seems to be reading a textbook, but he is devout in appearance. He admires Yang Rui and even becomes a "fan Shu" from PCR.

There are too many new technologies developed on top of PCR technology.

For example, if we calculate the social benefits of X-linked enzyme assay and Y-specific DNA probe, they are at least 10 billion yuan. If you don't limit the cost, you can support at least 10 tattoos, smoke, drink, swear, but know you are a good girl.As far as the derivative value of the whole PCR technology is concerned, it's not a problem to feed the women who have failed in several army groups.

It can be said that PCR technology opens a new chapter in biology, just as the microscope opens the door to the micro world, PCR technology opens the door to the era of DNA.

However, in the genetic engineering laboratory, Ouyang is the only one who really has an international vision.

Ouyang Shi has not only participated in international conferences for many times, but also can read and publish English documents. This skill is common among middle and high-end scholars at Peking University. However, it is very rare to see it nationwide.

Therefore, in the whole laboratory, Ouyang Shi is always the most excited.

He knows Yang Rui very well.

For example, PCR technology, most of the technicians in China don't know what it is, and a small number of technicians at a certain level can't really understand the power of it.

No matter Gu Qiang from Shandong University or researchers from other local universities, although they know that they have done a great job in the Genetic Engineering Laboratory of Haidian District, they still stay in the idea of "filling in the gap". They always feel that Yang Rui is imitating foreign technology, but they can't realize the profound changes of technology.

Now there is no internet forum, no instant messaging software, even no mailbox. Everyone's information sources and insights are limited to the people around, which is helpless.

At this time, Yang Rui had no time to talk about the contribution of PCR. He waved his hand and said, "don't brag, let's do our work first. If we do our work well, we will naturally have achievements. Professor Ouyang, your side is ready. "

"Oh, I forgot." Ouyang Shi said and took out the box of PCR instrument.

Today's PCR instrument is the first version improved by Yang Rui, which is much simpler than the original version of PCR. There are all kinds of automatic power-off protection, which is basically equivalent to simplifying the work of cooking spicy hot to cooking rice in an electric cooker.

Ouyang Shi paid a little attention and made two records, even if he completed a group of experiments and made records.

Gu Qiang is even more dismissive of this.

Yang Rui ignored him and turned his attention to Ouyang Shi's experimental table.

The sex identification of embryos is the last big hurdle they face, and it is also a big hurdle they have not touched before.

No matter whether it's for mouse or rabbit embryo transfer, Yang Rui doesn't arrange the part of gender identification. After all, not doing gender identification will not affect the operation of the whole system.

However, when it comes to commercial bovine embryo transfer, it is impossible to break the barrier of sex identification.

When doing experiments, no one cares how many male and female mice you have transferred. However, if Zhongmu has spent money on the embryo transfer of 1000 cows and got four or five hundred bulls, it must be crazy. What's the use of a bunch of bulls? Even if you want to slaughter and sell them abroad, the number of four or five hundred is not enough for channel construction. As for breeding, it's 85 years now. Of course, the precious Simmental cattle have to be artificially inseminated. It's easy for a strong bull to sow one million.

Therefore, as far as the whole commercial embryo transfer system is concerned, embryo segmentation, cryotransplantation and sex identification are equally important. Of course, the technology of superovulation is also very important, but because there is no difficulty, it has not been paid attention to by Yang Rui.

The main obstacle of sex identification is the high requirement of early identification.

In fact, sex identification of 8-cell embryos is only the minimum requirement for commercialization of sex identification. For 10 years at most, the minimum requirement for sex identification of bovine embryos by using Y chromosome specific DNA sequence is only 2 cells.

That is to say, once the fertilized egg is doubled, sex identification can be done.

In this way, the cost of commercial embryo transfer is naturally reduced again. Because it is the way of artificial insemination, the time to get the fertilized egg is determined, and the time to double to two cells is also determined. In this way, after a round of identification, the embryos that do not meet the requirements can be quickly removed, and the embryos can be used again with the least loss, Thus, the efficiency of commercial embryo transfer is greatly improved.

If it is a large biotechnology service company, it can also directly take out embryos and distribute them according to the requirements of different customers, so that modern animal husbandry is under the control of industrialization from beginning to end.

Because of the advantages of this technology, sex identification is no less important than embryo segmentation and cryopreservation.

However, this technology is only just developing all over the world.

No way. It was not two years since Yang ruicai developed PCR technology. Before the invention of PCR technology, both cytogenetic analysis and differential analysis of embryonic development rate had relatively low accuracy and high cost, which was not popular with ranchers.Correspondingly, after the emergence of PCR technology, sex identification before embryo transfer has become very popular, not only by ranchers, but also by biological companies. Correspondingly, sex identification before embryo transfer has become a trade secret among biological companies.

Technologies such as embryo segmentation and cryotransplantation were all developed around the 1970s. By the end of 1985, even if the patent period had not expired, they would be widely used.

Gender identification is different. PCR, as the latest technology, promotes the development of a variety of new technologies, and these new technologies, no matter whether they apply for patents or not, are naturally kept secret.

In this way, Yang Rui naturally can't explain his direction guidance with the data from abroad, but has to overcome this technical difficulty step by step.

Original research is basically equivalent to complicated research.

"Think of it as a trump card." Yang Rui comforted himself in this way, and then put himself into the huge amount of information and data.

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