Chapter 1015

With the participation of Professor Ouyang Shi, the experimental progress of frozen embryo transfer is inexplicably accelerated.

Gu Qiang's operation speed was originally very fast, now it is more agile.

I don't want to make way too far.

Yang Rui guesses Ma's mind.

In the laboratory, it also entered the most natural silence period, only the sound of moving metal and the brief and powerful conversation.

This is the normal situation in the laboratory. Everyone feels familiar and comfortable.

For a long time, Gu Qiang stretched his waist and said, "most of the semi embryos have been processed. Can we catch up?"

"It's about the same time. Let's thaw at the same time." Yang Rui answered, turned to other scholars and explained: "our experiment today, as long as it is to prove that the frozen embryos can be used for embryo transfer, let's not investigate the freezing time. In order to save your time, we will start to thaw the frozen embryos now. Wang Lei, prepare the equilibrium solution. "

Wang Lei, who was in charge of the assistant, immediately got busy.

This is also a time when scientific research can play a big role. Fine operation is also required for processes such as configuration of equilibrium solution. If there is no suitable experimental assistant, Gu Qiang can only do it himself, and the progress will be more than doubled.

In theory, there is no difference between thawing immediately after freezing and thawing after a period of time.

In this way, it is enough to prove the success of frozen embryo transfer.

The lab is busy again.

However, Zhang Feng was even more uncomfortable. He couldn't help but say, "this is too much fun for you. Why don't you do so many key steps separately?"

Embryo segmentation, embryo freezing, embryo thawing and embryo transfer can be regarded as different experimental steps, but their problems are also very real.

However, Yang Rui did not regard frozen embryo transfer as such a huge project, not to mention that it was a problem solved by foreign biologists in the 1970s. When Yang Rui was in graduate school, any dilapidated laboratory repeated such an experiment, and even could be used as a graduation thesis for Postgraduates in genetics, How can Yang Rui get up with the idea of being careful and doing things step by step.

Waste of time is a waste of life, Yang ruitou did not return the way: "look at the results, if we can not succeed, we will push back the steps."

"I'd like to see the result." Zhang Feng doesn't believe Yang Rui at all.

Ouyang Shi recognized the obvious distrust. He turned his head, looked into Zhang Feng's eyes for a while, and said, "Professor Zhang, you have such a bad idea. I doubt whether you have basic judgment ability."

"My judgment? What's wrong with my judgment? "

Ouyang Shi tut tut two, said: "this is Mr. Yang Rui's project, how can it not succeed? If you don't even have such a judgment... I suggest you learn from Comrade Gu Qiang and divorce. Without the burden of your family, your judgment is expected to gradually recover. "

Zhang Fengqi's face is flushed. Who has no judgment ability?

Gu Qiang was even more angry and said, "I didn't divorce for work!"

Ouyang Shi asked: "what is that for?"

Gu Qiang brain blank for five seconds, slowly tunnel: "for science."

"Well said!" Ouyang Shi season praise, way: "you this words, say of my heart surging ah, if not I beat my wife, I also divorced."

……

The third day of the experiment of frozen embryo transfer was a success.

After announcing the transplant the next day, Yang Rui went home and had a good sleep.

The next day.

Later, Yang Rui rode his bicycle to the ion channel Laboratory of Peking University.

When you don't get to the place, you'll see a lot of people.

Yang Rui frowned.

At the entrance of the ion channel laboratory, sure enough, there were at least 50 or 60 people gathered here, so that most of the originally empty road was blocked, and the passing students had to carefully pass by the corner, leaving an impression of awe.

There are only seven or eight Peking University professors with names.

Yang Rui didn't dare to take the big one, so he got out of the car and pushed it all the way. Then he said hello one by one: Professor Fang, Professor Li, Professor Zhang, Professor Qian

At the gate, the familiar figures of Professor Cai and President Liu also appeared.Needless to say, in front of the masses, are to see the experimental results.

Yang Rui has some helplessness, but he is also a bit cool. At the beginning, when he did research on potassium ion channels, how many people in the world believed that he could do it? Except for the colleagues in the laboratory, basically No.

And now?

Yes, people are still not optimistic about his frozen embryo transfer, but even if it fails, people have to watch it.

Not to mention the academic failure, it still has extraordinary value. In fact, it has exceeded the existing academic level to promote frozen embryo transfer to the stage of transfer.

If Yang Rui adopts the phased experimental method, he can at least announce a partial victory and then solicit funds.

In front of him, Professor Cai saw Yang Rui and said, "member Wu Hongbo is coming too. Let's stand and wait."

"Ah... Why is he here?" Wu Hongbo is also an academician of Professor Cai's Department, but he won't come to Beida at leisure. Compared with Yang Rui, a scholar with the title of member of the academic department can be more than three times busy.

Professor Cai chuckled twice, looked at Yang Rui with funny eyes, and said: "member Wu is from the Institute of genetics, Chinese Academy of Sciences."

Yang ruimeng patted his forehead and said, "I'm so busy."

"Don't let him poach people." Professor Cai whispered again.

"Yes, you think so." Yang Rui really admired. If he could think of this level, he probably had done this kind of thing.

In a short time, Academician Wu Hongbo arrived, accompanied by several other scholars in their 40s and 50s.

Without introduction, Yang Rui recognized two of them.

He recently read a lot of genetic engineering journals, and scholars with names and surnames also have to put their heads up in journals.

There are so many people. When they greet each other, they are in a fluster again. When they are clear, please come in, and there will be no more in half an hour.

Yang Rui sighed, and he had to thank everyone for coming. In fact, it was the same. If it wasn't for the success of his face brush, there would not be a good thing for all the sages.

Of course, the success of the experiment is a good thing, the failure of the experiment is not necessarily.

This is also the reason why Yang Rui didn't publicize it. However, Yang Rui can't control the good thing that he wants to launch satellites in public and control the scale.

Fortunately, in front of the experimental platform, Gu Qiang and Ouyang Shi are calm. They are people who have seen big scenes, but they are not frightened by more than a hundred people.

In contrast, Wang Jiancheng, a veterinarian from the Veterinary Research Institute of China animal husbandry Corporation, was slightly nervous.

The purpose of inviting veterinarians from the Veterinary Research Institute of China animal husbandry Corporation is to prove that the operation difficulty of embryo transfer has met the requirements of commercial grade. Of course, the premise is that embryo segmentation, culture, preservation and other technologies are all completed by the genetic engineering laboratory.

However, for China animal husbandry Corporation, as long as the goal can be achieved, they don't care how to achieve it.

"Dr. Wang, please give the recipient rats general anesthesia first." Yang Rui asked Wang Jiancheng to do the previous steps first. Even if he made a mistake, he could change a group, which would not affect the final success rate.

Wang Jiancheng was stunned for a moment and quickly agreed. He took down the sodium isopentobarbital from the test stand and quantitatively absorbed

In the Veterinary Research Institute of China animal husbandry Corporation, Wang Jiancheng often does scientific research, which is why Yang Rui chose him. But Wang Jiancheng has been doing scientific research all his life, and has never operated under the gaze of two academicians and dozens of famous scholars. He can't help feeling a little stiff.

Fortunately, the requirements for his operation are not high, and the husband of the mouse will not make medical trouble because of his injection method.

After a while, the recipient mice were all in place.

Yang Rui immediately ordered, the road“ The middle abdominal line incision exposed the bilateral uterine horn

This part is also the operation of Wang Jiancheng.

Then, it was Gu Qiang who took over and moved into the semi embryo with Wang Jiancheng.

The experiment was completed by one group.

The process is a bit flat, but the atmosphere is always tense.

At noon, it was all over. Yang Rui was also slightly relieved and said, "in the afternoon, we will be able to determine the proportion of live births."

The quiet environment in the laboratory was swept away, and the scholars immediately threw out the words they had saved for a long time. However, the contents were not all supportive of Yang Rui.

"It's not likely to have a live fetus."

"It's not that easy."

"Even if there is no live fetus, this experiment is worth referring to.""If there is no live fetus, we should start over. In my opinion, Yang Rui's experimental conditions are not enough."

"It's a bit too fast to split and freeze embryos at the same time. At that time, we won't know which side has problems. "

"That makes sense. Now there are few laboratories that can do embryo segmentation, and then frozen embryos..."

Although Gu Qiang is an experimental operator, he is not optimistic about Yang Rui's experimental design, and has no confidence in the results.

However, Gu Qiang still couldn't help saying: at least live one, at least live one

As a direct operator of the experiment, as long as one mouse survives, he will gain more fame than before.

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Today, PK, the champion of the challenge, starts to walk. I'll carry the flag. Can anyone help me, brothers! Yesterday, I found an artifact in Longwu 2. I thought it would be a great success at last. Later, I learned that everyone has his own artifact... I don't need to get up early and brush the equipment any more!

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