Chapter 737

"Recombinant DNA methodology has led to the development of DNA or gene cloning. By the end of 1970s, the method of inserting short DNA fragments into a plasmid or virus chromosome and then replicating in bacteria or eukaryotic dead pigs has been relatively mature. Later, I will elaborate on how to implement gene cloning and the reasons for the breakthrough in molecular biology caused by this technology."

Yang Rui said here, pausing for a moment, and then said, "the paper I did before, which studies the structure of potassium channels by cloning mutant genes, is based on this design."

The experts and professors who were ready to move at the bottom were all silent.

The publication of domestic scholars in "cell" is extremely rare in the whole country. Yang Rui's paper on potassium ion channel, up to now, is unknown to the domestic biological circles.

If such papers fall to a middle-aged scholar, they will be worshipped as legends.

If you throw it to an academician, it's like painting a new layer of gold.

Yang Rui wrote this "cell", of course, the impact is stronger, but, Yang Rui's identity, slightly delayed its power, let it not explode, but slowly spread the power.

However, the classroom has always been a place where teachers have full power.

This is true for professors in the classroom and for part-time teachers in the classroom.

Yang Rui's thesis, at this time, is enough to frighten all the ghosts.

At the same time, as a high-end application of DNA recombination technology, the paper of potassium ion channel is academically difficult.

All the teachers who wanted to talk now bow their heads and take notes obediently.

They are all people who have read Yang Rui's papers, and some even know about the scientific research competition between Yang Rui and Richard. Therefore, no one wants to question Yang Rui in terms of DNA recombination technology.

A cell is hundreds of thousands of words, plus a single digit icon at most. The information contained in it is something that ordinary scholars can't figure out in their lifetime. When someone has published a journal with an impact factor of 1.0, it means that the average number of papers cited in this journal in the past two years is 1.

In the SCI index, impact factor 1.0 is not the bottom line. There are a large number of journals with impact factor 0.5. Moreover, these journals are good journals in the journal world.

What is the impact factor of the top journals CNS? Generally speaking, it means more than 30 and less than 40.

In general, the average number of papers published in cell, nature and science is more than 30 and less than 40.

What is the unusual situation? This is how Yang Rui's papers came out.

As a technology destined to rewrite the history of biology and the history of human science, it is reasonable and unexpected that 2000 papers were cited in just a few months.

And its citation value is now equivalent to 50 CNS level papers, 2000 or even 4000 ordinary SCI level papers.

Even compared with similar excellent super top papers, PCR is also a record excellent.

Among the top papers also published in nature, the ones with higher citations are generally 200 to 300, and few of them have heard of 400 or even 500 citations. In the field of science, this is not a simple doubling problem. There are only those biological laboratories in the world, each of which has its own field. It is not a very wide range of theories, with 100 citations, It's all over the world.

But the end point of PCR is far from 2000 citations.

This is a technology destined to rewrite history. The global biological laboratory is going to understand it, use it and quote it regardless of field.

Yang Rui stood confidently on the platform, facing the creaking camera, waiting for someone to debate with him.

He waited a full minute.

For a long minute, the classroom was silent.

"No one has any objection?" Yang Rui's clear voice echoed in the classroom.

With the modesty of Chinese people, Yang Rui juxtaposed his PCR technology with classical genetic theory and recombinant DNA technology, and called it the third generation technology. Some people should have called it "arrogant".

A minute later, however, no one did.

Yang Rui waited another minute, but no one did.

Yang Rui sighed with regret.

Although carrying 2000 citations is enough to crush any enemy in academic circles, it's a bit lonely to drive straight in without any enemy.Lonely for defeat? Not yet.

After all, this is a lecture hall. The lecturer can freely tell his own views and cognition. It has no authority.

If it was an international conference, the people's Congress who raised their hands would probably break through the sky.

However, the lecture hall is still a formal occasion, although it is not as solemn as an international conference.

Yang Rui is more willing to present his genomics in a more comprehensive manner.

To write a book and establish a theory, a theory needs information as thick as a book to prove itself.

"Since there is no objection, I will talk about the research methods dedicated to genome on this basis." Yang Rui turned around and wrote down the words "genome research methods" on the blackboard, which instantly exploded the audience.

Compared with Yang Rui's banner for PCR, the direct technical guidance is obviously more exciting for scholars.

Of course, the students don't understand the excitement of the experts and professors around them, and they look at the blackboard at a loss.

Finally, Wang Dongming, who had always been bold and conceited, couldn't help but raise his hand and said, "Mr. Yang, where can we see the textbook of this class?"

……