Chapter 595

The original PCR process is complex, which is equivalent to high-precision spicy hot.

A bunch of DNA is selected from the test tube, and then it is placed in the test tube. Next, a bunch of test tubes are put into the high-temperature water bath pot. After picking it up with a stopwatch, they are put into the next high-temperature water bath pot, and then into the low-temperature water bath pot for dozens of seconds.

Different time and temperature will have different results, and the same action is often repeated more than six times seven times. Before there is no high temperature resistant polymerase, new polymerase needs to be added between each action.

This kind of PCR technology, just like the computer technology in the 1940s, is complex and precise, with low fault tolerance rate. When people see its prospects, they also see its shortcomings, and think that the shortcomings will accompany them.

There will always be shortcomings, but the degree is different.

Yang Rui decided to make the shortcomings of the first version of PCR less, so that you can see the convenience of PCR, more prospects, less attention to details.

This may be a difficult choice for other companies. They don't know how much resources they will consume, how long they will spend, and how much risk they will take... After all, it's easier said than done to make it convenient. It's better if they have inspiration. If they don't have inspiration, they have to be impatient.

But for Yang Rui, it's too simple to make PCR technology a little more advanced, just a little bit.

Hands on is not Yang Rui's strong point. When Yang Rui thought about the result, he asked Zhang Xuetong to give him a description of the requirements, and then said, "I think the most troublesome problem can be solved by changing multiple water bath pots into one.

Zhang Xuetong thought with his head covered. For a moment, he was in a daze and said, "several water bath pots have different temperatures. How can I use one? Heating and annealing, which can not be less

Instead of talking about the solution, Yang Rui said, "let's talk about the result first. If several water baths are changed into one, is the operation simple?"

"That's for sure. First of all, you don't have to move the test tube around. In the past, holding the stopwatch to pick up the test tube would be like holding the stopwatch to change the temperature." Zhang Xuetong said to think about it and asked, "can the temperature of the water bath change?"

"Yes." Yang Rui affirmed: "if I have a good impression, there should be a kind of water bath pot that can control the gradient temperature."

"The water bath pot that controls the temperature gradient... Is the water bath pot that can quickly change the temperature?" Zhang Xuetong quickly understood and immediately frowned: "it's not easy."

"It won't be too difficult. I guess there are finished products. If not, let's find a way to change them." This is a very simple improvement made by modern science and technology. Yang Rui doesn't know who invented the earliest version of the automatic temperature changing water bath pot, but at the latest in the early 1990s, this kind of water bath pot liberated the heavy manual labor.

Precise problems are controlled by machines, and the most complex and challenging parts are solved.

In fact, the original PCR instrument was so simple that a water bath pot with variable temperature could replace multiple water bath pots.

As for the water bath pot, it is just a cylinder that can boil water and maintain the water temperature.

After the technology that can win the Nobel Prize has finally become a finished product, it is not much more complicated than a spicy hot stand. The modified PCR technology is simpler, and the setting operation is slightly more complicated than the oven.

Countless people will say when they see the final version of PCR: why didn't I think of it.

Zhang Xuetong felt the same way. Touching his chin, he said, "if there is a fast automatic temperature changing water bath, your method is really good... I think it's reliable, then I'll check the literature?"

"Well, you look up the literature. I'll have people search for patents to see if any companies have done it." Colleges and universities are active promoters of network and computer. For colleges and universities, the cost of information inquiry is extremely high, and it is not enough without inquiry.

Literature can be checked through the library. For example, Peking University Library has a special person to do this. Patent search in large patent offices is also responsible for a special person, but the fee is expensive.

In fact, as far as the current price is concerned, it is Yang Rui, a researcher like a person in charge of a laboratory, who has the money to inquire about patents. Ordinary researchers have neither the money nor the desire to do such things.

It's no use thinking about it, especially for the researchers in Colleges and universities outside Beijing. They have a lot of trouble in looking up literature. It's really not easy to write a paper. Some people spend only a few thousand yuan a year, not to mention inviting a special company to look up patents. The money they call is very tight.

However, Yang Rui is a generation used to outsourcing. By the time he went to graduate school, many experiments were not completed in a single laboratory. For example, if you want to use a microscope with tens of millions of magnification, the laboratory can't afford a machine, and the school doesn't have one, so simply ask a unit with such a machine to take photos. After all, no one likes to borrow machines and solve things with money. We are not willing to talk.In addition to the lack of instruments, there are also many experiments that are difficult to operate. Some experiments take more than half a year to learn, or it costs a lot of resources to learn, and teachers are lazy to teach. It's better to directly outsource them to outside biotechnology companies. The cost is low, and we don't have to bear the reputation of "peeling".

Since the beginning of the decade, many teachers who can only recruit postgraduates but not doctors have become more and more used to doing experiments by themselves. Graduate students in the 1980s are all new people. Their main job at the undergraduate stage is to take the postgraduate entrance examination. They seldom go to work in the laboratory. However, the work in the laboratory is becoming more and more difficult. Many teachers with master's degree director's brand think it's troublesome to teach others, so it's better to do it by themselves, just as graduate students are bottle cleaners, It doesn't matter what you want to do and what you want to do.

In the next few days, Zhang Xuetong tried to make a self-made water bath pot with fast changing temperature.

This thing is not difficult, and there are not many strong patent barriers. Most of the related patents have expired, and the patent owned companies have not continued to do similar research and remedy.

After all, before the era of PCR, the water bath pot that can quickly change the temperature was also dragon slaughtering, which could be applied in a small range.

The beauty of science lies in this. Some scientists will inadvertently create a dragon, and some scientists will inadvertently invent dragon slaughtering. The most interesting thing is that scientists who invent dragon slaughtering often invent before the dragon.

When the original PCR was made, there was a fast warming water bath, but no one thought of combining the two. The private company where the PCR patent is located has not considered it, and the invention company of the fast warming water bath may not even know about PCR.

Under the instruction of Yang Rui, Zhang Xuetong made the PCR instrument almost two or three years in advance, and with the help of patent lawyers, he applied for a lot of patents in the name of Huarui laboratory.

The Chinese in the lab don't care about patents. They just think the new PCR instrument is quite easy to use. They praise Zhang Xuetong one by two.

"Save at least 50% of your strength." Li Wenqiang's hands are sour when he does experiments. He especially likes new equipment.

Although it looks a little ugly, it's really easy to use.

Zhang Xuetong smiles like a flower. He only thinks that the value of life has been reflected to the greatest extent. He even uses joking language: "it's more than 50%, I think it's at least 80%

"80% of the achievements, anyway, there is no reward." Li Wenqiang didn't like it.

Duan Bo, who was selected with them at the same time, joked: "80% is 80 points, which is not worth rewarding."

"How can 80% of the energy saved be 80 points? Can 90% of the energy saved be 90 points?" Zhang Xuetong looks aggrieved, but he still keeps the experimental record on his hand.

Wei zhenxue said silently: "if my son scores 90 in the exam, I have to beat him to death."

Li Wenqiang and Zhang Xuetong are speechless. How dare we all be your sons?

Wang Xiaoyun, as the only female researcher in the laboratory, was afraid that the world would not be in chaos and asked, "Lao Wei, how many points would you give up beating?"

"That's 100 points, of course." Wei zhenxue's natural way.

"100 points? It's over. " Wang Xiaoyun laughs.

Wei zhenxue does not understand: "why is it finished?"

"Because zero is not divisible." Li Wenqiang shook his head.

Wei zhenxue suddenly responded, "Oh," and said, "what I'm saying is, saving 80% of my strength is 80 points, saving 90% of my strength is 90 points. If I want to save 100 points, I have to save 100% of my strength. In this way, I can't get 100 points."

"Right." Zhang Xuetong replied casually.

"Yes." Wei zhenxue sighed and said, "it's lucky that you two tortoise sons have fallen into my hands. Otherwise, I have to discount your legs every time I pass the exam in my nineties."

Li Wenqiang was stunned for a moment. He felt that he had suffered a loss and did not know how to refute.

……