"If I can go abroad at my own expense, please help me contact the report quota of an international conference." It's no use for Yang Rui to go abroad by himself. All kinds of international conferences need invitation letters. High level academic conferences have higher specifications and it's more difficult to make reports at conferences.

If Yang Rui didn't have a cell article, now the article is good, don't even think about it.

If there is no invitation, just like many non-governmental scientists waiting outside various international conferences, they can only hold up signs to protest.

Professor Cai knew that Yang Rui was rich, but he could only shake his head regretfully and said, "only when there is an invitation to an international conference can there be a place to go abroad. In recent months, there are no vacant places. If you can get there yourself, I will not prevent you from going abroad."

"I'm afraid it's too late." Yang sighed.

"Let me ask for you. Maybe there are still places in the chemistry department or the school. The chance is not big." Professor Cai shook his head again, saying that he could not help.

Of course, he didn't really recognize the value of PCR. If he knew that PCR could win the Nobel Prize, he didn't need to. In 20 years, as long as he could win the title once, Professor Cai immediately took off his clothes and traveled around the second ring road of Beijing barefoot for 13 minutes, begging for a meeting place.

However, most of the academic achievements, when they were still seeds, could not be seen in the future.

Moreover, sitting in the chair of the Department, Professor Cai also hopes that Yang Rui can put Peking University in his signature.

After comforting Yang Rui, Professor Cai sent Yang Rui out.

Yang Rui can only go back to the laboratory and ponder over it. He made two overseas phone calls between them, but no miracle happened.

During the international conference in Beijing, he met several foreign professors and maintained contact with them, but their ability was not enough to make Yang Rui jump in the queue.

At this time, Yang Rui is deeply aware of his lack of contacts.

Fortunately, if "nature" is published smoothly, it won't be a big problem.

At that time, Yang Rui's achievements include one cell and one nature. Although many students and teachers from top universities in Europe and America can publish papers in top journals, few can publish two.

This will make it easier for academia to recognize his contribution to PCR.

However, if it can't be published in nature smoothly, and there is no meeting to make a report... Yang Rui's tangle is almost endless, and it is difficult to find a solution.

At this time, he couldn't stop thinking again: if I start PCR later

However, the fact is that it can't be any later. No matter how late it goes, although the Americans haven't registered the patent of PCR, the idea of PCR should also come out.

At that time, when a Chinese freshman competes with a white American doctor for the right of discovery, Yang Rui will lose.

By the end of the week, Li Wenqiang and others had made part of the "golden rule". To be more precise, they were familiar with it.

To do the "golden rule", the most arduous work is to run glue, and most people will do a mess at the beginning of running glue. The swimlane runs crooked, the strip is missing, and the fancy is chaotic. In a word, all kinds of unprecedented mistakes may appear in the first few times of running glue, but they can't see the right one.

When Yang Rui was in graduate school, running glue was a basic activity of biological research, just like liquid chromatography was a basic configuration, no student did not learn it. However, in the 1980s, top universities and famous universities with weaker departments did not have relevant technology and equipment. Li Wenqiang and others did not know how to run glue before, so they practiced in Huarui laboratory.

As the number of experiments increased, the film naturally became more and more beautiful, and the PCR reaction results gradually became more reliable.

The background reaction disappeared, the signal in the negative control disappeared, and all kinds of unexplained exotic phenomena gradually disappeared

In Yang Rui's opinion, running glue is similar to washing clothes and folding quilts, both of which belong to the skill of practice makes perfect. The first time a child who can make quilts runs glue, he still has to start from a mess, and the first time a child who can make quilts, We have to start from a mess.

The difference is that you can learn how to make quilts everywhere, so the cost of running glue is a little high.

In order to complete the experiment under the "golden rule" as soon as possible - which is the key to prove the feasibility of PCR to others - Li Wenqiang and Duan Bo are working overtime on their own initiative.

Zhang Xuetong needs to provide more oligonucleotides, and has to spend the night to improve the equipment.

The money flowed out like water.

In just one week, Sinorama's account was filled with $30000 more for materials, and lawyers spent more.Yang Rui is really worried by his accumulation.

Fortunately, biological research, the company that produces DNA synthesizers, has given them a batch of accessories and materials for free, reducing the cost of the laboratory. The company is completing the improved DNA synthesizer according to Yang Rui's improvement suggestions, and there will be a refund when it goes on the market.

It's just that the water is far away and thirsty.

Anxiety and research results are growing at the same time. Yang Rui also stayed in the laboratory at the weekend, hoping to push forward the project as soon as possible.

In the afternoon, an unexpected visitor appeared.

"Dean Liu?" Yang Rui opened the door and was surprised. He's in the Hua Rui lab, not the ion channel lab.

Dean Liu came here by bike. He was sweating. He wiped his sleeve and said with a smile, "I'm here to see you. Can I get in?"

"Oh, come in, come in." Yang Rui quickly let president Liu into the yard, and put his bicycle against the wall, and said: "there are few people in the laboratory, so we don't have a special parking shed."

"It's beautiful." President Liu looked at the courtyard of siheyuan style, took the initiative to stand in the middle of the tree shade, said with a smile: "autumn tiger is very hot, your laboratory is cool, what a big piece of land, bigger than the school laboratory?"

"It covers an area similar to the laboratory of the school, but there is no neighbor in front of it, so there is room for expansion in the future. An extra yard has been built nearby. Now it's used as a warehouse or something. I plan to apply to the district for a bigger yard after the Spring Festival. It's better to build several staff dormitories. Researchers are running around and wasting time. " Yang Rui gossips with President Liu, speculating about his purpose.

"This point is good, this point is good..." director Liu nodded with a smile, looked at the direction of the laboratory, asked: "the project you showed Professor Cai is made here?"

"Oh, yes." Yang Rui didn't say much.

Liu is not afraid of embarrassment, but when he didn't see Yang Rui's indifference, he continued: "Professor Cai took it to the meeting."

Yang Rui looked at him hopelessly and asked, "did you pass?"

"Professor Cai put forward two plans, but they failed."

Yang Rui shrugged unexpectedly. Since Professor Cai didn't agree with him face to face, the meeting didn't make much difference. Of course, this is not to say that Professor Cai is not willing to push this matter, but the driving force he is willing to give. He thinks that the driving force that should be given is far from enough to push Yang Rui's request.

Of course, Professor Cai can arbitrarily give Yang Rui a place, but this is not the case.

President Liu waited for a few seconds and asked Yang Rui to ease his mood. He said, "don't be unhappy. I'll tell you the truth. Time is too tight. I really can't vacate the quota. The quota of going abroad won by others is not easy. I also have my own job. Are you right?"

"I can understand. It was an invitation."

"You understand best." President Liu smiles, observes Yang Rui's expression, and says: "your project is quite good. At the meeting, not only president Cai, but also everyone has given high praise. We don't want you to lose the opportunity for a project like this. If you want to publish a paper in nature, we are willing to support it. But as the saying goes, to be famous, your project is similar to that of Peking University, Basically no contact. You are a student of Peking University, right, but if you don't sign your name in the paper, our school can't force calculation, do you think so? "

President Liu didn't wait for Yang Rui to reply, and then said: "we also know that your project is supported by Huarui company. How do you think about this? You can contact Huarui company for us and say that we are willing to pay one third of the funds and ask for a signature."

Yang Rui raised his eyebrows: "this project has cost nearly a million dollars."

Director Liu's eyebrows also jumped, and immediately laughed: "where can there be millions of dollars? We estimate that 600000 dollars should be enough, not counting the instruments."

60 Ten thousand dollars is estimated according to the funds that Huarui can give Yang Rui and the time consumed by the project, not the cost of the project itself.

If Yang Rui did PCR from the beginning, 600000 US dollars would not be enough, but Huarui did not spend 600000 US dollars.

Yang Rui said noncommittally: "600000 dollars is more than 600000 dollars, but if you calculate by 600000 dollars, you also need 200000 dollars. When did we have so much money?"

"The money can be given to the school. You have to let a first author come out."

Yang Rui suddenly realized that this was an academic transaction.

20 If you buy an article of "nature" with ten thousand dollars, you will use the money of the state. Naturally, you will not lose money.

In any university in China, however, leaders with strength are probably willing to pay for it.It's a good deal.

In fact, in terms of the current domestic environment, it's not easy for us $200000 to write a piece of nature.

It is Yang Rui who has a good history and has published top journals that people are willing to take this risk.

For a moment, Yang Rui was moved. If it's a coenzyme Q10 related paper, fill in a contract, Yang Rui will certainly follow. After all, this kind of technical paper, after all, will belong to the digital part of the results. One more co-author, one less co-author, no impression.

However, this is not a paper on coenzyme Q10.

Yang Rui shook his head and said, "President Liu, you don't have to say that I can't do this. It's not only about the signature, but also about the patent."

"Patents are simple." President Liu seemed relieved and said with a smile, "we certainly don't want your patent. In this way, I'll write a statement and let the office stamp it with a steel seal to say that we don't want your patent, OK?"

Yang Rui curled his lips and said in disbelief, "what if the superior department doesn't recognize your statement?"

If the patent is $300000, Yang Rui will believe the integrity of the superior department. Yang Rui doesn't want to have a relationship with the relevant departments.

Liu Yuanchang further advised him. In fact, he didn't have a bad heart. He just wanted to kill a few birds with one stone and felt that Yang Rui didn't have any loss.

Yang Rui didn't want to listen any more, but he suddenly interrupted him and asked, "where did he get the quota for the leader you mentioned?"

"What?"

Yang Rui was also inspired and asked, "where did the leader find the quota? I can't sign my name to Peking University. Huarui laboratory can't get through. However, I'm willing to pay for a new place. "

"It's not about money," Liu said with a smile. "It's about the quota for going abroad, business passport, daily subsidy for going abroad, and the quota for the international conference. It's not about how much money you spend. It's not easy for people to say."

Yang Rui also laughed and said: "you may be talking about Chinese standards. If you go abroad, it should be international standards. In international standards, local tyrants are best to use them."

"What?"

"One for two." Yang Rui put up two fingers and said, "I can get two places for going abroad. There is no place for this international conference, but it can be a visit to a company or university. You can help me change the places, and I'll give you a refrigerator ticket."

84 In 2008, families with better conditions in Beijing had televisions, but refrigerators were not necessary. President Liu didn't use them himself, and it was also a wonderful gift.

President Liu dunxian hesitated.

"They can go abroad together." Yang Rui's language is bewitching. It would be great if it could be done for thousands of dollars.

Finally, director Liu shook his head and said, "this one can't do it. He won't just go abroad. That... The leader I'm talking about is holding the project in his hand, so he is sure to get the quota. Otherwise, would you like to find a project from Huarui? "

"The project doesn't work." Yang Rui is a little disappointed. Huarui laboratory is his. The project costs tens of thousands of dollars and hundreds of thousands of yuan, which is too high.

"I'll think about it again." President Liu really wanted the refrigerator ticket. After thinking about it for a long time, he said, "how about this? I'll introduce a person to you. You can talk to him about two places. Maybe it's feasible."

"Who is it?"

"Those who come back from studying abroad want to go back all the time. He didn't go abroad to attend the conference, but if you want to make a report at the conference, you can't help it."

"I see. Let's go and see." Yang Rui also did not expect to do the project will make so much trouble, but it is not possible to continue.

……