Chapter 565

Su xiankai and fan Zhenlong are doing their own experiments, and their eyes scan Yang Rui's desk from time to time.

Since entering the "ion channel laboratory", this is the first time that they have seen Yang Rui do experiments seriously.

Although the person in charge of the laboratory does not have to have practical operation ability, just as an officer does not have to have strong individual combat ability, it is obvious that officers with strong individual combat ability are more likely to be welcomed by soldiers.

Moreover, Yang Rui is a little younger, just like a young officer, who is always vulnerable to the provocation of soldiers.

Su xiankai and fan Zhenlong are still newcomers, and they are recruited by Yang Rui. They dare not challenge Yang Rui's authority, but they have no problem observing secretly.

With their observation, both of them were obviously disappointed.

Yang Rui's operation level is too general.

Of course, this is a high standard judgment. In fact, Yang Rui's experimental level has improved a lot. However, since he became the director of the laboratory, people's expectations for him have also increased.

For example, Xu Zhengping, who is a middle-aged uncle, still does not have his own independent laboratory. However, his experimental level has been trained for more than 20 years. Although many movements are not up to standard due to incorrect learning, he has been doing experiments for 20 to 30 years. The nonstandard movements also make Xu Zhengping's experimental operation not smooth.

In contrast, Yang Rui, even if some movements are very standard, still can't hide the raw under the hand.

"Slow."

"Too slow."

This is the common idea of Su xiankai and fan Zhenlong.

As for Xu Zhengping, he has long been used to Yang Rui's experimental actions which are not fast enough. This time, what he does not believe is Yang Rui's experimental plan.

Take a few days to do DNA sequencing. Are you kidding?

Gene sequencing technology began in 77, when there were chemical degradation methods for DNA sequencing, and Yang Rui's current dideoxy chain termination method. However, no matter which method it is, it is not easy to use this technology for DNA sequencing, with high error rate and low efficiency.

Of course, no matter how low the efficiency is, it's better than the small fragment sequencing method invented in 65. The latter is just like the potassium ion channel project by Yang Rui. It's not only by luck, but also extremely difficult.

Xu Zhengping believes that in those top laboratories, some researchers who are super familiar with DNA sequencing may indeed spend a few days to make plasmid DNA sequencing. However, ion channel laboratory is not a top laboratory, it is not even a provincial laboratory, and Yang Rui is not a researcher who is super familiar with DNA sequencing

in fact, There are no researchers in China who are super familiar with DNA sequencing.

Gene sequencing is indeed a very important biological experimental tool. If the "human gene sequencing" project is the peak of biology in the 20th century, gene sequencing can be regarded as the most important experimental tool in biological history.

But throughout the 20th century, China has always been slow.

65 Sanger invented the small fragment sequencing method and completed the determination of 120 nucleotides of E. coli 5strna. At the same time, the country was busy burning books and beating teachers, so there was no time to learn.

77 In, practical DNA sequencing methods appeared, and China was busy reprinting books to recruit teachers, so it was too late to learn.

As an associate professor of Peking University, Xu Zhengping came into contact with DNA sequencing, which was just a few years ago. He did a complete experiment once in all, so as to experience the most important technology of modern molecular biology. Before his project involves gene sequencing, he probably won't do it a second time.

Most of the domestic researchers are like this. In order to save resources, when graduate students learn these things, they are also theoretical learning.

Xu Zhengping thought with his cerebellum that even if Yang Rui had done DNA sequencing, he would have done it once and a half.

I know from his movements. It's strange. With this technique, we can do DNA sequencing in a few days.

Xu Zhengping deeply believes that Yang Rui is losing money, let alone relying on it to get floating funds. These expensive reagents are all in vain.

Yang Rui didn't notice the sight of the people around him.

This experiment is really difficult for him, and it needs concentration.

The assistant he Quangui and others can do such things as configuring buffer solution and annealing mixture. Even centrifugation and ethanol cleaning can be done by the assistant. But when it comes to adding solution, then running electrophoresis, or heating and cooling on ice, it's all Yang Rui's work.When Yang Rui was a student, the automatic sequencer for DNA sequencing came to the second generation. The automatic sequencer can't be said to be fully automatic, but it can replace more manual steps.

Before the government stopped, ordinary pregnant women could do gene sequencing and prenatal screening.

But in 1984, the current DNA sequencing technology did not even have the first generation. Lao Mei may not be able to make the first generation of DNA sequencer until next year. If the Chinese can afford it, it will take another two years - just like apple mobile phone, if you can afford it, you still have to wait for factory production.

Without automatic DNA sequencer, manual operation is very complicated.

DNA In the final analysis, sequencing is to analyze the base sequence of DNA fragments. Adenine is a, thymine is t, cytosine is C, guanine is g. These four letters circulate in various forms, forming the basis of organisms. DNA sequencing is to determine the sequence of these four bases.

Its principle is very simple, but it is not easy to do.

Because the base is invisible, to sequence, we need to make it react, and it is the right reaction.

It's the devil's step for theology to accurately make the required base react step by step.

Yang Rui's pace was very slow, slow to the extent that Xu Zhengping could not see it. Hours later, after Yang Rui's electrophoresis finished, a clear gel appeared in front of several people.

"Strange beautiful..." fan Zhenlong was older and more social. He wanted to flatter Yang Rui, but he finally found a chance. Although he flattered Yang Rui less, he got Yang Rui's smile.

Su xiankai's reaction was a little bit slow, and then he said: "it's very beautiful..."

No matter how young Yang Rui is, when he was in charge of the laboratory, the lifeblood of all the people in the laboratory was in Yang Rui's hands. From funding to publishing a paper, everyone has to be restrained by Yang Rui. If you meet a better person in charge of the laboratory, you can get more funding, less boring tasks, and less trouble when you publish a paper. If the person in charge of a laboratory is of poor quality, not only the funds are small, but also the achievements are required. What's more, some person in charge of a laboratory not only have to be the corresponding author or even the first author, but also dare to sign the author's name to someone outside the laboratory to do human relations or even pure transactions.

When Su xiankai and fan Zhenlong first arrived, they couldn't figure out Yang Rui's temper, but at least they knew that it was always right to follow Mao.

Yang Rui recorded the results and continued to repeat the process.

This time, for a few hours, I still got a beautiful gel and got a round of praise.

Yang Rui repeated it again and again.

From morning to evening, Yang Rui did not stop except for eating. In the afternoon, he even added a group of experiments.

Su xiankai and others have nothing to praise, but Xu Zhengping's eyes are straight.

Actually each time directly got the accurate result!

In the age of manual experiments, this is very difficult.

What's more, it's a brand new DNA sequence. If it's a repetitive experiment, it's much simpler to repeat it according to other people's answers, just like fan Zhenlong and Su xiankai's repetitive DNA sequencing, but the brand-new DNA sequencing will always produce some unwanted answers.

Just the last step of running, often encounter the situation can not run out, and then fall short.

Yang Rui's every step is slow, but there is no mistake, which makes Xu Zhengping almost dare not think.

Can not imagine the scene, now appeared in front of him.

It's like a final exam. Only one student scored 100 points in the exam. You can't be right if he said he was plagiarized. If the exam is true, there are correct answers. Look, Yang Rui is doing the unique sequencing of the S5 fragment of thermophilic bacteria in Tengchong in the world. Let alone foreign researchers don't have thermophilic bacteria in Tengchong, there are, They have not sequenced and published public papers, which Xu Zhengping, who often checks the data, is very sure.

"It's beautiful." At eight in the evening, Yang Rui looked at Xu Zhengping and made a clear gel.

Yang Rui said with a smile, "it's just a peddler."

"It's a groundbreaking experiment. It's no use just doing it by hand." Xu Zhengping didn't believe him.

In fact, Yang Rui said something wrong. He extrapolated the sequencing according to the answers in his mind. Of course, he was accurate every time. At this time, he naturally would not refute. He pretended to be modest and then laughed and said, "make another group, and I'll have a rest. If you want to go back, go back first."

"Don't worry. Let's all have a look and learn." Fan Zhenlong has another flattery."You all have families with rooms. If you don't go back with your wife, what's the meaning of my experiment?"

"Spiritual support." Fan Zhenlong's answer was brief and forceful.

"I'll do it for you. I've finished my experiment and used up all the reagents I prepared today." Su xiankai is more direct.

Many reagents, such as buffer, need to be used and prepared on the spot. Generally speaking, they should be configured when they come in the morning, and the number of reagents should be determined according to the progress. When they are used up, the experiment should be over. Of course, some demanding experiments can't even be stored for a few hours, so they can only be matched in between.

Su xiankai has prepared more reagents, but he is not ready to do it at 8 pm. Now he wants to continue to do his own experiment, and he has nothing to do.

Yang Rui gave him a place and said, "then stay with me for another two hours. I'll finish the sequencing these days. It's good to get tens of thousands more, isn't it?"

"Of course, tens of thousands of yuan is good, but it's really hard to finish it in a few days." Su xiankai told the truth.

Yang Rui said with a smile, "I'm tired, but I should be able to finish it."

Su xiankai couldn't help saying: "the S5 fragment of thermophilic bacteria in Tengchong has a total of 126 base pairs."

126 A base pair, which is relatively short. Yang Rui made five in a day, which is also very fast. But he knows that he can't finish the project in a few days by using primary school mathematics.

In fact, it has been flying fast. In foreign countries, many laboratories have sequenced a gene chain and tested it for one or two years. During this period, all kinds of wonderful problems have been encountered.

It's amazing that Yang Rui is doing so smoothly today, but Su xiankai can't flatter him so much if he wants to say that it's smoother and faster.

Yang Rui didn't have to explain. He just went on.

No one noticed, but now his proficiency has increased.

……