Chapter 562

Yang Rui is quietly planning for his PCR project. His assistant only sees him reading literature and doing experiments by himself. He doesn't know what Yang Rui is doing.

At this point, Yang Rui has been like a normal person in charge of the laboratory.

In fact, Yang Rui's work in the past six months has been preparing for PCR.

First of all, the expanding scale of Huarui laboratory. The full name of PCR is polymerase chain reaction, which is a molecular biological technology.

In 1984, anything associated with "molecule" was just like "nano" in the 21st century and entered the high-tech field.

High is the high cost, new is the new development of new instruments.

Since the construction of Huarui laboratory, the cost of equipment can exceed US $1.5 million, which is Yang Rui's dividend for more than two quarters, equal to one third of Yang Rui's income.

Any rich person who comes here will be scared to pee when he sees such expenditure.

This is pure investment. It's a mystery whether it can be recovered. Although Yang Rui has proved himself once, it is still hard to say whether he will win the second scientific research competition.

Fortunately, Yang Rui doesn't have a boss, which makes him free to prepare for PCR. Otherwise, academician CAI of Peking University thinks highly of him, but if Yang Rui says that he wants to buy equipment with 1 million US dollars, that's no problem.

In the scientific research field, those with low status should do projects with less resources. Otherwise, they will be overstepping their capacity. Without the life of the princess, you can't expect the tutor and the headmaster to treat you like a father. Even if the princess disease can't be cured, it's necessary to control it as much as possible and improve the quality of life.

In addition to the soft and hard conditions of Huarui laboratory, Yang Rui's preparation for the PCR project was to make the high temperature resistant polymerase.

But his preparation, and did not tell anyone the need.

Huang Mao, who made the high temperature resistant polymerase himself, didn't know what the high temperature resistant polymerase was for.

This is a situation that people engaged in research often encounter. Anyway, if they do it, it will be an achievement.

Also because he was not in a hurry to use it, Yang Rui asked Huang Mao to postpone his paper, and he agreed without hesitation.

And in the heat resistant PCR, it's the real PCR.

PCR What can be done is complex, and what can be done is simple. Complex PCR is the simplest for users. The so-called "full-automatic PCR" will be used by students 20 years later. Just put the material in, press two keys on the machine, and then spit it out later.

Simple PCR is complicated for users. Polymerase is added to each cycle. The reason for adding polymerase is that each cycle of PCR has a denaturation process. By means of high temperature, the double strand of DNA is untied, which is the prerequisite for replication.

Before the high-temperature resistant polymerase was found, the general polymerase could only withstand the temperature of 37 degrees. When the DNA was double stranded, the polymerase was finished.

Normal DNA is made up of two strands twisted together. Polymerase is used to copy the two strands separately. The process of putting them together is the process of DNA replication.

In this case, DNA is like a girl covered with white phosphorus and dressed in a safety suit.

The polymerase responsible for replication is like a naked man.

The process of replicating DNA requires mating.

If a man wants to mate, he must first tear open his safety suit.

If you tear open the safety suit, the white phosphorus with low ignition point will burn instantly, and the man with low heat resistance level will be burned to death. You need to add another man later, because the white phosphorus has burned out, and this time the replication is successful.

However, this is only the first set of cycles.

When the mating man entered the second cycle, he was obviously burned again by the brand-new burning chastity pants, so a third man was added.

There are disadvantages in constantly adding men. First of all, the corpse is difficult to handle, and the appearance and smell are not good, which affects the subsequent mating, accumulates more, and even takes up space.

Therefore, the best way is to find a man who can withstand high temperature. If the "burning chastity pants" cycle appears, it will not be affected.

For a practical PCR, only when a man with high temperature resistance is found, can this technology have practical value.

Muris's PCR suffered a loss in this respect.

Yang Ruiguang is to avoid this one, can save half a year of time, but he also has a waste of time.He doesn't have the right gene in his hand.

To be exact, it's Yang Rui who has no sequence analysis of gene fragments.

Human genome sequencing refers to the sequence analysis of human gene fragments one by one.

In order to copy the gene fragment, we must first know what is in the gene fragment. That is to say, to know all the information about the girl in safety suit, has she ever learned judo? What's your height? How powerful is it?

Only after the sequence analysis of the gene fragment is determined can the primer be determined, and then the appropriate polymerase be sent for replication. For example, when you meet a judo girl, you can send ^ *, not ^ *. Although the latter has something to do with judo, it is not an appropriate compatibility, and it should be strangled without hesitation.

When muris was doing PCR, he was in a big company, and others did the work of cloning gene fragments themselves. Naturally, there were all kinds of gene fragment analysis.

Yang Rui doesn't have this condition, even in China, so he has to find the right gene in the published papers.

This work is easy to say and difficult to do, mainly for fear of paper fraud or stupid papers written by stupid people.

The so-called fear of ten thousand is just in case. There are a lot of false or wrong papers in China, the United States is not new, and neighboring Japan is also exposed every year.

If it is an ordinary paper, Yang Rui will not study the authenticity of other people's papers, and can almost use it.

But PCR is a project worth 3 billion US dollars. Once it is successful, I don't know how many pharmaceutical companies and biotechnology companies will stare at it. Yang Rui can't make his paper based on an unstable paper.

If you don't verify the papers that are not made by the laboratories that have close contact with you, there will be any problems. For the $3 billion project, it will be a gap to attract flies.

If it's a gene fragment analyzed by his own company, Yang Rui can also review the experimental process. If it's a gene fragment analyzed in China, Yang Rui can also ask academician Cai - if the other party is willing to show it the best, if not, he can have a minimum judgment.

However, Huarui laboratory does not have such a reserve.

You have to repeat the experiment yourself.

However, the work of gene sequence analysis can be handed over to the "ion channel laboratory" of Peking University.

According to Yang Rui's design, gene analysis was originally one of the main objectives of the official laboratory.

The future "human genome project" will cost hundreds of billions of dollars. Moreover, all the gene sequences obtained by the human genome project will be open and unprofitable.

No matter how rich and powerful Yang Rui is, he can only pretend to be pure in front of such a plan.

Of course, genes other than the "human genome project" are still available for profit, such as the genes of T. rex. You can find something to study, and no one cares about you.

But anyway, these are plans for the future. For the moment, repeating the sequence of several gene fragments is a pure basic research, and it's a good way to train soldiers by leaving it to the "ion channel laboratory".

After carefully selecting several plasmid fragments, Yang Rui immediately started several small projects to clone the target fragment sequence in the ion channel laboratory.

The newcomers, Su xiankai and fan Zhenlong, are eager to prove themselves and are not surprised to take over the job.

Xu Zhengping was a little surprised that Yang Rui repeated the experiment so much, but he had a lot of work under his hands and didn't care about others.

The only problem is that there are not enough experimental dogs.

After more than a day's work, Su xiankai couldn't help finding Yang Rui and said, "Professor Xu has his students' help. He Quangui and Zhao Pingchuan, the public assistants of the laboratory, don't come every day. They also have some fixed work. I need at least one special assistant to do the experiment."

"That's right. I'll arrange someone to help you first. You can type a report. I'll go to Professor Cai for help." Yang Rui's laboratory is also in operation. Two researchers are in place, and it's time to recruit experimental dogs.

"Me too." Fan Zhenlong raises his hand at the other end of the lab.

"One person, one full-time assistant, I see." Although Yang Rui brought their establishment to Peking University, it will take a long time for the school to arrange teaching positions for them. During this period, they naturally have no way to find experimental dogs by themselves, only Yang Rui can arrange them.

However, looking at his own independent laboratory, Yang Rui also has a different idea.

He specially asked them to make a report, found a time when academician CAI was in the office, ran over and handed in the report, and said: "I had set up a joint laboratory with Hedong University of Hedong Province, called Jielikang Hedong university laboratory, with the help of Jielikang, in order to do coenzyme Q10 research. A lot of students in the laboratory are selected from the Biology Department of Hedong University. Many of them have been doing experiments for two or three years. I wonder if I can find two people from the laboratory of Jielikang Hedong University and transfer them to my ion channel laboratory. "What Yang Rui wants to do is naturally to transfer Yao Yue.

Academician Cai didn't know why, so he said strangely, "can they agree to transfer people from Hedong university? Teacher or student? "

"I want two students who have been doing experiments for a long time, so that they can get started without any further training. All the students who can do experiments in our school have their own ideas, and the rest are either not here, or they have to be carefully selected... Another one, I think fan Zhenlong and Su xiankai are all transferred from other schools. When they first arrive, senior students may not be willing to cooperate with them. I am familiar with all the students in the laboratory of Jielikang Hedong University. " Yang Rui has plenty of reasons.

Academician Cai pondered: "there was no such precedent before."

Yang Rui said with a smile: "it's only a few years since the resumption of the college entrance examination. If you have done it, there will be a precedent. Who dares to say anything. Isn't there that exchange student abroad? Let's have a one-way communication. Hedong university should not object to it. "

It's a little troublesome to talk about. Academician CAI can't agree with an ordinary teacher. However, the status of "ion channel laboratory" in academician Cai's mind is not limited to that. The cooperation problem raised by Yang Rui has made academician Cai pay more attention to it.

The college students are still arrogant. The students in Peking University are naturally more arrogant. Fan Zhenlong and Su Xiankai are Yang Rui's chosen tune. They are not old and have no special achievements. If they encounter two Peking speaking students who can not speak, they will delay the implementation of the examination.

The harmony of the laboratory can't be seen or touched, but it can't be ignored.

Thinking of this, academician Cai asked, "how do you want to operate?"

"Can you ask Jericho to come forward? We sent two students from Jielikang Hedong laboratory to Peking University to exchange information for one or two years. We don't need to change the student status. Let's make arrangements here. We should treat them as our own students. We should have a class and a meeting. We should focus on the laboratory and ask Jielikang to give us some tuition and miscellaneous fees... "

"What if you're not satisfied?"

"Then call back directly."

"Well, to win the consent of students and their parents, write a written consent form."

"No problem. If you come to Peking University, there's nothing you don't like." Academician Cai touched his beard and laughed.

……