Chapter 476

First of all, Yang Rui quickly browsed the paper in his mind to make sure that he would not say more, less or even wrong.

The development of biology is very fast. Different from other disciplines, the rapid development of biology is not only the forward change, the horizontal change, but also the change of the concept itself.

In other words, the same word, it is now such a meaning, in a twinkling of an eye may be another meaning.

How fast is it? If a doctor of biology pursues romance and accidentally creates an explosion when he proposes to the goddess, he will be jailed for five years for causing death by accident. When he comes out again, the child of the goddess is just in the third year of junior high school. This is not the part of change. This is the normal situation. The part of change lies in, The doctor of biology department is now going to tutor the goddess's children in biology. His knowledge is old and backward, so he has to tutor himself first.

The world is changing so fast.

To make a comparison, the mathematical Euclidean geometry was invented more than 1000 years ago. Newton had five axioms when he was in junior high school, and Lu Xun had five axioms when he was in junior high school. In the 21st century, Riemann corrected the last one. As a result, it was too difficult for Junior high school students to learn mathematics, not to mention junior high school students.

Physics, chemistry, even metallurgy and architecture are commonly used knowledge thousands of years ago, at least hundreds of years ago.

Only biology will be awarded the Nobel Prize this year, and the textbook will be changed next year.

However, if you use those advanced words before the mainstream academia can recognize them, the situation will become very interesting.

This two-way choice meeting carefully prepared by Professor Cai is likely to become a huge discussion field, completely deviating from the original purpose.

Yang Rui thought about it for about a quarter of an hour before he said: "in fact, I published several papers before I did potassium channel. When I did potassium channel project, I also did a few extra small experiments. I didn't write them in this paper because they are not related, but they are really very good subjects."

"Oh? What is it? " Professor Cai presided over the meeting. When Yang Rui talked about potassium channel, he nodded slightly.

Starting from the potassium channel is the most correct way.

Yang Rui sat up straight and said, "it's the study of proteins in potassium channels. However, it's not the current mainstream G protein, but the interaction between proteins."

"You go on."

"Protein is the basic functional unit of life activities, and all physiological changes are inseparable from the interaction between protein and protein. When doing the potassium channel project, I found that we not only need to understand the structure and function of a single protein, but also need to understand the functional process of protein's internal action... "Yang Rui stopped for a moment and explained:" the research on the structure of repeated potassium channels may be a simple and clear choice, but it's just the structure of potassium channels, Soon there will be no way to go, and the level of papers that can be published will be reduced step by step, but the relationship between proteins is different. In an experiment I did before, I didn't find any relevant research results, just because of the time, I haven't made a careful comparison and formed a paper. "

In the last sentence, Yang Rui is just talking nonsense. He just looked for some papers. However, interacting protein is an important association research of potassium channel, and Yang Rui did read a lot when doing the project of potassium channel.

For interacting proteins, Professor Cai is noncommittal, but seizes Yang Rui's words and asks: "so, the laboratory you lead will not do projects on the structure of potassium channels?"

"It's not impossible to do it at all. If we have enough money, we can do it in advance." The biggest difference between leading a lab and joining a lab is that one is the boss, coordination and command are more important, and the other is the migrant workers, who should participate in the lab on the spot.

The boss can have more than one laboratory at the same time, and the workers usually can only work in one laboratory.

For Yang Rui, if someone is willing to write top-level papers in his own laboratory, he has no reason to object.

As for guidance, it doesn't take much time. When he comes home for the new year, Huang Mao doesn't just lie in the laboratory doing experiments, and his progress is not affected too much.

Good guidance is like poking the window paper. The problem is not how strong the force is, but the direction and distance.

Most of the laboratory leaders, for the guidance of the paper, are only limited to guess. The purpose of keeping a large group of experimental dogs is to let them realize guess. Sometimes it is a clever method, sometimes it can only be foolishly wrong.

However, Yang Rui has done a batch of potassium channel projects, so it is very easy to guide again. Although it is not as easy as the chemistry teacher guiding students to do standard experiments, it is not much more difficult than walking a dog.

Yang Rui's answer has also been recognized by everyone, and many people show their satisfaction.Yang Rui's answer shows that the student's brain is clear, not only his IQ, but also his Eq.

This is a very important point for professors and associate professors who are not familiar with Yang Rui.

The only reason why Yang Rui can sit here is that there is a "cell". If he breaks away from the potassium channel and his first achievement, and makes a complete comeback, it will be rootless Ping. Few people will build a laboratory with him.

So far, all the people present are actually thinking about co constructing the laboratory, and they often put Yang Rui in this position.

In everyone's opinion, this is already an excellent treatment.

As for the independent laboratory mentioned by Professor Cai, it is more like a picture cake. It is much more difficult for Yang Rui to get it than to build a laboratory together.

Even Professor Cai himself hesitated.

In fact, in terms of achievements and papers, more than half of the scholars here are not as good as Yang Rui.

"Cell", "nature" and "science" are Bibles in the field of scientific research. They are hard conditions that can't be harder. There are no more than 50 articles published in China a year, and there are no more than 10 articles in biology.

Although most of the papers with this number fall under the name of top universities or institutions such as the Chinese Academy of Sciences, Peking University or Tsinghua University, most of the professors still have no chance or ability to publish papers on CNS.

For example, Professor Tang Jiji, although his impact factor is more than 10. 0's syst-biol (Systems Biology) has published many papers, but cell is cell, and Sb is always two worlds.

Of course, the influence factor of the papers published by Professor Tang Ji Ji is low, and there are also factors of the times. Before the reform and opening up, most Chinese scholars preferred to publish Chinese journals, and at this time, Chinese journals are still alive.

On the other hand, the current Chinese universities are not only influenced by the factor theory.

However, no matter how you look at it, "cell" is also an amazing power. If Yang Rui is not a student but a teacher, even a junior assistant who has just graduated from college, he is also qualified to lead a laboratory independently.

Now it's three years away, and it's hard to turn around.

However, Professor Cai gave Yang Rui a chance.

This is the best effort he has made, free from the influence of tradition, inertia and secularity.

But for now, Professor Cai doesn't want to follow Yang Rui's words and says, "you say there is plenty of money. How much do you think it will cost?"

"200000 US dollars plus 3 million RMB." Yang Rui's own potassium channel project cost more than 600000 US dollars, and now he still has a pain in the flesh.

This kind of project can't get any money back, and there's no good way to apply for a patent. Even the research based on it is basic. The research based on it is the technical research that can be applied for a patent.

However, just as movies have experimental films, art films and commercial films, most of the things that go for awards and style are pure losses.

For example, PCR, a project with both fame and wealth, is very rare.

If it wasn't for the sake of accumulating reputation, Yang Rui would not have invested in his own paper on potassium channel.

In the future, it is better to leave such research to the National Laboratory. It's better to let the research dog spoil the national tax than the official ape.

Dogs are human friends.

Professor Cai was not surprised by Yang Rui's figures and asked: "is that enough? How long will it take. "

"According to the proficiency of the experiment, and luck, three months to six months, of course, more money must be faster."

"If it's 100000 US dollars, what about 4 million RMB?" Professor Cai doesn't think Yang Rui wants more, but he is willing to save a little foreign exchange.

Yang Rui said with a smile, "no way," and said, "most of the reagents have to be imported."

"If I can write such a paper in half a year..." Professor Cai pointed to the "cell" on the desk and said, "I will approve the funding."

"Thank you, Professor Cai. I'll go on."

"We don't have many laboratories with 200000 US dollars and 3 million RMB at a time." Professor Cai reminds Yang Rui.

"I understand." Yang Rui knows that if the original project is redone, many associate professors and even professors will be willing to join, because they are very likely to win the honor of the first author or co-author of CNS.

But for Yang Rui, the nutritional value of such a laboratory is too low. If you want a new project next year, it will be more difficult to have another meeting.After a little brewing, Yang Rui said, "let's talk about protein-protein interaction first. I learned a little about the detection methods of protein-protein interaction. There are probably several methods. I think that after the establishment of a new laboratory, we can start with the detection methods."

"Yes." Professor Cai nodded, the detection method is purely technical, relatively stable, but also can obtain a certain amount of results.

Yang Rui cleared his throat and said, "I think what I can do is, first, protein affinity chromatography; Second, affinity imprinting; Third, immunoprecipitation; Fourthly, glutathione transferase precipitation experiment; Fifth, chemical crosslinking; Sixth, fluorescence resonance energy transfer method. "

Speaking of this, the professors who originally listened to Yang Rui's talk with a smile slowly became serious.

It's just a list of detection methods that many people on the scene can do. It's nothing more than memorizing or reciting. Some of them can still be remembered, let alone memorizing.

However, Yang Rui's skillful listing shows that at least he didn't speak freely.

And in the attention of the public, Yang Rui and no stage fright to say three: "seventh, surface plasmon resonance technology; Eighth, phage display technology; Ninth, yeast two hybrid or multi hybrid... "

When it comes to the seventh, many people, including Professor Fu, look unnatural. The reason is very simple. They have heard the terms of surface plasmon resonance, but they don't understand the specific content.

Professor Fu immediately raised an objection and said, "what you just said is that the new laboratory can do, and these tests can be done?"

Detection method is not only detection method, update more advanced detection method, often represents more accurate and fresh conclusion, in reality, is more and better paper.

It's a lot more meaningful than the elusive meaning of protein.

When Yang Rui was studying, these detection methods were not new. They belonged to common laboratories. He did not talk nonsense and said directly: "surface plasmon resonance technology is to obtain the specific signal of biomolecular interaction through the dynamic change of SPR angle, that is, resonance angle, in the process of biological reaction. We need to use computers and software to analyze the ligands and molecules to be detected on the chip, which can be used to detect antigens and antibodies, receptors and ligands, proteins and nucleic acids, and the interaction between proteins and small molecules is also very convenient. "

Professor Fu opened his mouth and could not refute. Because he didn't understand, he could only look to both sides like asking for help.

Lu Yueping, who once recommended Xu Anqing to Richard, coughed and said, "the principle of surface plasmon resonance technology is right. I used it when I visited the UK. However, the computer technology used in this technology is quite complicated. Can you do it?"

"I have used similar computers in Huarui laboratory. If you are interested, I can show you on the spot." Yang Rui doesn't have any empty words. Although today's computer is complex, Yang Rui doesn't find it very difficult to learn. In fact, the academic special software of later generations is not simple. The wonderful human-computer interaction and friendly interface never belong to the research dog.

Now, just using the application computer to do experiments has a very academic flavor.

Professor Cai is also slightly moved. Yang Rui's youth seems to have become an advantage at this time.

……