Chapter 461

In the middle of the small courtyard, which is paved with bluestone bricks, is a pool of hot spring water, which emits a little gas.

The moonlight, falling on the hot spring water, seems to have a light cyan rise.

Liu Shan seems to slide down into the water, like a spirit in the night.

Slender waist, proud hip, in the air to draw a arc, impressive as if time is still in general.

Yang Rui is holding a pile of things in his hand, a little hesitant.

"Give me the eggs." Liu Shan propped up and waved from the hot spring.

"Oh." Yang Rui takes two steps and hands the egg basket to Liu Shan.

At this time, he saw Liu Shan's all black one-piece swimsuit, as well as her red cheeks. He didn't know if it was the hot spring that reflected the red.

"I'll make hot spring eggs, and you cook instant noodles." Liu Shan rowed twice under her feet and laid the eggs under her feet.

Yang Rui looked around and hesitated about where to cook noodles.

Hot spring water can boil hot spring eggs, not hot spring instant noodles.

Looking at him, Liu Shan was a little funny. She pointed to the back door and said, "it's tied there. Go and fetch water."

Yang Rui suddenly realized that he went to the back door. It was a bolt and there was no lock.

Open the back door, the street lamp on the path is very dim, there are no people or animals. Most of the sanatoriums are old cadres. The busiest time of the day is 90 o'clock in the morning. After dinner, one by one goes to bed earlier.

Although there are people like Yang Rui who come here to play, there are not many. Yang Rui gently closes the back door, goes back to the room in three or two steps, brings boiling water and bowls, and the hot spring eggs are just cooked.

"While it's hot, I'll cook the noodles." After soaking in the hot spring for a while, Liu Shan also felt tired. She leaned on the edge of the pool, tore open the instant noodles and began to put seasoning.

Yang Rui can still see the enchanting figure under the water with the help of Yu Guang.

Although I have seen countless pictures and influences, I feel very different when I see real beauties, real swimsuit beauties, especially when they are alone.

Yang Rui only felt that his body was extremely active, and all his cells seemed to be alive.

"I'm in the water, too." Yang Rui took off his coat and slipped down the hot spring in his shorts.

Liu Shan turned her back to him. She couldn't see her expression, but she was stiff for a while.

A few minutes later, the instant noodles were soaked well, and the two of them lay by the hot spring and ate side by side.

The water is warm, and the eggs boiled in the hot spring are fresh and tender.

Yang Rui is sure that this is the most delicious instant noodles he has ever eaten.

……

It is the third day that Wei zhenxue and others have returned to the sanatorium.

The mountain roads in Tengchong are more difficult to walk than they expected. Several people suffered some skin injuries more or less. In addition, it is also very difficult to transport nearly 100 kg of samples separately.

Sun Ruyue, as the youngest experimental dog, has played a great role. He is like an Eskimo Dog, carrying more than 30 kilograms of things on his own. He is quite shy and says, "I'm used to doing farm work in my hometown."

"Have a hot spring egg." Yang Rui patted sun Ruyue on the shoulder, then raised his thumb and said, "well done."

Sun Ruyue was praised fatigue subsided, instantly from eskimo dog smile into Samoye, said: "this is what I should do."

"Have a good night's rest. I'll be busy tomorrow." Yang Rui used to be an experimental dog. If he came out with his tutor, he would have to do the hard work of taking samples.

Turning around, Yang Rui began to examine the samples.

When the sample is in the hot spring, it is just ordinary silt, ordinary spring water and ordinary soil. But when it becomes a sample, the word "holy" should be put in front of its name.

The sample is like a nun of the Holy See. From the moment it is put into the box, it can no longer be defiled by others until the owner of the box, such as a bishop or a researcher, opens it.

"I'm going to do some tests now. You'll have a rest and help tomorrow." Yang Rui can't help but start. Although the samples are kept properly, any storage device can not resist the time, and the best way to keep them is always to use them as soon as possible.

At the very least, Yang Rui should make sure that these samples are not contaminated and thermophilic bacteria are active. Otherwise, if he returns to the capital and finds that there are not enough samples, he will be in trouble.

Isolation of thermophilic bacteria is only the first small step in countless steps. Thinking about the distance between himself and PCR, Yang Rui's heart slowly raised a sense of urgency.Although in the future, there is nothing unusual in the evaluation of PCR technology by countless people - it is true that this technology that can replicate DNA infinitely is more like a delicate technological unification than a technological innovation, but the technology that has nothing unusual does not mean that it is light and easy to lift.

It takes half a year for biology graduates to write a graduation thesis. Many people copy the experiments in the book and can't do it. Then they cry like a female driver staring at the parking space on the side

PCR The application of technology is so extensive that it is destined to be exquisite in design.

The dinosaurs in Jurassic Park were cloned by PCR technology. The so-called DNA can be detected with just a little blood. It doesn't mean that there is very little blood needed for DNA detection. In fact, there is PCR technology, which can copy the DNA contained in a little blood infinitely, so as to facilitate testing.

Before PCR technology, in other words, in 1984, DNA replication was very troublesome.

PCR Technology is revolutionary in application.

It took more than three years for the original author of such a revolutionary achievement. He was a lazy guy, but he was also diligent sometimes, but that was in the absence of competition.

What would he think if he knew that Yang Rui had similar research?

Will it speed up?

Or give up.

Yang Rui doesn't know. He has to prepare for the worst.

As for the choice of not doing PCR and not cutting Hu, Yang Rui would not consider it at all.

If, from the perspective of personal interests, the technology with Nobel Prize is not realized, what is it?

If we take the moral high ground and realize PCR one or two years ahead of time, it will bring countless miracles of life. Biology is not the crude art of farming, mining or machinery. It is a discipline directly related to life. Realizing an important biological technology one year earlier means that countless new drugs may be invented one year earlier, that countless patients may be saved one year earlier, and that countless people's lives may undergo earth shaking changes

Of course, Yang Rui never demands others, or his own, with a high degree of morality.

However, he is more or less "ordered by heaven" feeling, when it comes to doing business, the spirit is extremely focused.

Liu Shan looked at Yang Rui quietly for a few meters away, then with a smile, rolled up her sleeve and joined the ranks of experimental assistants.

Wei zhenxue a look, do not rest, said: "we also help."

"You're going to have a rest. So is Xiao Sun. Everyone is going to have a rest. Tomorrow is busy." Yang Rui shook his hand, lowered his head to take samples, and said: "I'm not polite in the laboratory. You all go to have a rest. I'll do a few groups of experiments to have a try."

Wei zhenxue hesitated for a moment and looked around at the green brick walls. He was not sure whether it was a laboratory or not.

In the end, however, it has to be counted.

Three hours later, Yang Rui found the first thermophilic bacteria.

Although I don't know if it is the kind I want, it at least proves that the sample is OK.

Yang Rui felt relieved and immediately went back to his room to sleep.

We must have enough energy to finish tomorrow's experiment.

The biggest difference between doing experiments and doing manual lessons is that thinking in experiments is essential.

Handwork class is a good step designed by others. At most, it is in the middle. When you are not sure, ask, "Gee, where did I just stick it?"

There are more questions about doing experiments, especially exploratory experiments. All previous experience can only be used as a reference.

For example, if the thermophilic bacteria in hot springs are archaea from the age of dinosaurs, the research steps are similar to those of a space-time shuttle after it arrived at the age of dinosaurs and scooped up a pool of water.

In fact, it's more complicated to study the thermophilic bacteria in hot springs, because it's not sure that they really came from the age of dinosaurs.

To do such an exploratory experiment, thinking at any time is necessary in the experiment, and thinking at any time means burning the brain at any time.

In the next few days, all the people in Huarui laboratory spent time with brain burn.

Every day, Yang Rui got up for breakfast and began to play with the centrifuge. In addition to having a hot spring in the afternoon, he completely required himself according to the standard of the laboratory.

Kang Hong came to see that their work was so tense that he was embarrassed to stay. After a long stay, he returned to Kunming.

Yang Rui didn't notice how long he stayed. His head was full of the speed of the centrifuge and the temperature of the medium.The treatment material obtained from the centrifuge is enriched bacteria. We don't know how many bacteria there are in a tube. These bacteria are directly smeared on the solid medium and cultured in 80 ℃ and 85 ℃ incubators respectively.

The common bacteria that can't survive in this environment will die naturally, and the ones that can survive will grow into colonies, which need to be cultured separately. Finally, a single colony will be selected for culture, so as to realize the separation and purification of the strains.

In short, what Yang Rui and his family got were all bacteria below their great grandchildren. The bacteria just rolled on the culture medium, gave play to their reproductive ability, and then died with the wind.

In this process, a lot of resources are consumed.

The culture medium of bacteria is more expensive than pure broth!

Every dish of culture medium, is a strong smell of money.

These bacteria, once grandchildren, must continue to consume resources to accept harsh living experiments. First, Gram staining, followed by whole protein analysis, the latter is to boil and crack the bacteria and gel electrophoresis.

After the electrophoretic map is obtained, there are still four or five steps to identify the thermophilic bacteria.

It sounds very complicated, and it's actually very complicated, but as far as the whole experiment is concerned, it's still very simple work.

Moreover, it is easy to make achievements.

After Yang Rui determined the genetic relationship of some strains according to the differences in protein fingerprints and compared them with the data in his mind, Yang Rui wrote down the name of his new paper: "the occurrence of extreme thermophilic bacteria with the highest temperature tolerance in China" on the return flight.

In fact, he has not determined the thermophilic bacteria in Tengchong, but he does not want to wait.

If "cell" is published smoothly, this paper just opens a series of letters, which can keep the heat of Huarui laboratory.

For research institutions, it is also very useful to keep the heat. Laboratories that can update their research results at a fixed time can always attract more attention from their peers, so as to spread their research results more quickly and get more reputation and funding.

For Yang Rui, who is running for a world-class project, every trace of attention can not be ignored.

……