Chapter 453

In the laboratory, Yang Rui felt his head and wrote a note on the paper, considering the next experimental goal. It seemed that he was idle, but in fact his brain was running violently.

On the contrary, Huang Mao and others worked conscientiously in front of the test bench, busy with re verifying the data, in order to complete the small paper work.

The relationship between the as like as two peas and the researchers is almost the same as that of the dogs and dogs who park the dogs in the park.

If cynical experimental dogs see this familiar scene, they will certainly talk about laboratory exploitation.

But that's how normal labs work.

And the more high-end laboratory, pure hard work and hard work, the more meaningless.

As the director of the laboratory, Yang Rui's work in the laboratory is mainly to ask questions.

In fact, raising questions here is to put forward one's own hypothesis or assumption by synthesizing existing scientific research achievements, especially the hot spots in the forefront of science.

For example, in the study of potassium channels, Yang Rui proposed the hypothesis that the structure and properties of ion channels can be verified by cloning mutant genes.

This can be said to be a very difficult and broad hypothesis. In order to realize it, Yang Rui put forward the second assumption: we can mutate the gene we want to mutate.

Similarly, in the second layer of ideas, Yang Rui has further detailed ideas, such as: for the organism of directional mutation, Drosophila should be preferred.

This step-by-step assumption and hypothesis seems easy, but in fact it is difficult, and it is also the primary condition for becoming the person in charge of the laboratory.

The work of Huang Mao and others is actually to solve problems and complete Yang Rui's assumptions.

In order to complete their own work, Huang Mao and others have to put forward their own hypotheses, which is also their self-training. The better the researchers do, the faster they can become independent.

As far as natural science is concerned, assumptions and hypotheses must be based on solid foundation, that is to say, pre research results already exist.

Of course, how firm and how set it is depends on the level of the proposer.

Low level, you obediently stand on a solid foundation, do what you can do, give human civilization a tiny force to move forward.

If the level is high, the requirements for the research results are low.

Classic examples, such as the Manhattan Project to build the atomic bomb, at the level of Oppenheimer at that time, he was not qualified to put forward this idea and hypothesis. It was Einstein who wrote to Roosevelt, and it must be Einstein, in order to start such a project without anything, even with a little experimental proof.

In fact, it was European scientists who pushed the project forward. They convinced Einstein to get the letter to Roosevelt.

Otherwise, according to the normal research mode, the Manhattan project can wait for 20 years of pre research, at least until someone makes enriched uranium, then it has the foundation to make a nuclear bomb.

But because of the state of war and Einstein's presence, the pre requirements of the Manhattan plan became lower.

Instead, the project is bigger.

What Yang Rui is considering is how big his new project will be and how big it can be.

What he wants to do now is PCR technology.

According to the time line, the original author of PCR, muris, should now start to consider the concept of PCR.

However, Mullis is a famous slacker, in a better way, free and straightforward.

Therefore, although the development of PCR is not difficult, murich took more than two years to complete it. It was only about 86 years ago that it was partially completed, and it took 87 years to complete it completely.

Now it will be safer to start PCR. However, Yang Rui has only one unpublished "cell". He will enter PCR immediately. It is no problem to do it, but he is not sure whether he can get the corresponding reputation.

Although the scientific community is a place to speak with achievements, it is after all a collection of people. There are not a few researchers who have received too much praise or have not received due rewards. Some of them have been recognized and even compensated in the next few years, but some of them may be buried in the pile of old paper.

It is true that Mullis is lazy enough, but the company behind him is not. On the contrary, next year, after the company has determined the certainty of PCR, it has invested a lot of resources, including many excellent researchers as its deputy.

After thinking about it, Yang Rui decided to seize the advantageous position first.

Although they do not have the corresponding reputation, they may not get the Nobel Prize - but who is sure that they will get the Nobel Prize.Einstein almost missed the Nobel Prize because the theory of relativity had not been confirmed, and finally had to use the photoelectric effect to win the prize.

Yang Rui's body, or muris's body, really depends on his life to win the prize.

Since it's life, it's no use suffering.

Yang Rui thought of this and began to write several key points of PCR on paper.

Yang Rui is also doing PCR projects, but he is not going to repeat the steps of muris in that year. To be exact, he can not repeat the steps of muris in that year.

Because PCR, in the final analysis, is a layer of window paper, easy to pierce, not easy to tear clean.

Yang Rui doesn't want to poke a hole and lead a pack of wolves.

Although it is likely to fall into the scientific research competition again, from Yang Rui's point of view, he still hopes that the later the scientific research competition occurs, the better.

He doesn't have the support of a biotech company with billions of assets behind him.

The most ideal way is that when Yang Rui has established an incomparable advantage, the later competitors will not have large laboratories to participate in the competition because of cost-benefit considerations. As for those scientific apes who always focus on hot spots to do experiments, it is impossible to put an end to them. These scientific apes eat rot, but they do not affect the work of the main laboratory.

"Well, let's start with that." Yang Rui's eyes are on the last step.

Although it is the last step, heat-resistant polymerase is the pre technology of practical PCR technology, and it is also a necessary material. If it is made in advance, it can speed up the later research and development, and reduce the cost.

On the other hand, this is a key technology, and as early as 73 years ago, there was related research.

Before PCR technology came out, it was not taken seriously because people didn't know what it could be used for, and the researchers themselves did it out of interest. In 1973, at the request of the instructor, a female student from Taiwan successfully isolated a thermophilic bacterium found in the hot spring of Huangshi Park, which is heat-resistant Taq DNA polymerase, Yang Rui doesn't even know if she has applied for a patent.

At that time, it seemed unnecessary. No one thought that it would eventually become a key part of a $300 million technology.

However, even if the other party does not have a registered patent, but has a paper in hand, it is also not allowed to rush to register. Yang Rui's brain turns fast, and immediately comes up with a new method: find a new thermophilic bacteria as the research object, and use a new method to separate the high temperature resistant polymerase.

Two researchers independently complete their own research, can independently apply for a patent, which is also a conventional means to bypass the patent barrier.

Yang Rui then rummaged through the relevant papers in his mind.

Thermophilic bacteria are microorganisms that live in high temperature. There are many kinds of them. There are also special researchers and laboratories to do relevant research. If Yang Rui does not prepare to join them, he may not be able to get out of them all his life.

He had to find a thermophilic bacterium that was well studied, make sure it could be used to isolate high-temperature polymerase, and then start related projects.

As far as the current scale of Huarui laboratory is concerned, it is not qualified to do the wild and costly trial and error method like the top laboratory.

More than ten minutes later, Yang Rui wrote down the word "Tengchong" heavily in his notes.

The handwriting is black and thick.

The thermophilic anaerobe in Tengchong hot spring is the first extreme thermophilic bacteria found in China. After it was discovered in 1998, Chinese scientists sequenced the whole genome DNA, thus becoming the first microorganism to decipher the genetic code in China.

Tengchong thermophile is a well-known bacterium in the Chinese biological world. Yang Rui has in his mind all kinds of studies on this microorganism, such as Archaea chromosome protein, new mechanism of bacterial spore formation, isolated extreme enzyme

It's natural to use it to isolate high temperature resistant polymerase.

"Let's go to the hot spring." Yang Rui suddenly raised his head and his eyes were shining.

……